تأثیر چهار هفته شنای هوازی بر میزان عامل نورون‌زایی مشتق‌ از مغز و گیرنده‌ی آن در بافت مغز موش‌های مبتلا به انسفالومیلیت خود ایمن تجربی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه خوارزمی، تهران، ایران

2 دانشیار، گروه فیزیولوژی ورزشی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه خوارزمی، تهران، ایران

3 استادیار، مرکز تحقیقات سلولی و مولکولی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی جندی‌شاپور اهواز، اهواز، ایران

4 استاد، گروه تربیت بدنی، دانشکده‌ی علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران

5 استاد، گروه فیزیولوژی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی جندی‌شاپور اهواز، اهواز، ایران

چکیده

مقدمه: Multiple sclerosis، به عنوان یک بیماری عصبی- ایمنی‌شناسی (Neuroimmunology) در انسان شناخته می‌شود. شواهد حاکی از نقش عامل نورون‌زایی مشتق از مغز (Brain-derived neurotrophic factor یا BDNF) و گیرنده‌ی اختصاصی آن، تیروزین کیناز B (Tyrosine receptor kinase B یا TrkB) در پیشرفت این بیماری است. تأثیر عوامل مختلف بر روی بیان این عوامل در مطالعات مختلف گزارش شده است. هدف از انجام این مطالعه، تعیین تأثیر چهار هفته شنای هوازی بر میزان عوامل نورون‌زای مشتق از مغز و تیروزین کیناز B در مغز موش‌های مدل حیوانی Multiple sclerosis یا مبتلا به انسفالومیلیت خود ایمن تجربی (Experimental autoimmune encephalomyelitis یا EAE) بود.روش‌ها: موش‌های C57BL/6 به 8 گروه 10 تایی شامل 6 گروه موش‌های مدل EAE (شاهد، شنا، دریافت اینترفرون بتا-1، شنا و دریافت اینترفرون بتا-1، شاهد تزریق، شاهد شنا و تزریق) و 2 گروه موش‌های سالم (شاهد، شنا) تقسیم شدند. از روز 9 بیماری، موش‌ها به مدت 4 هفته روزانه مقدار 150 واحد بین‌المللی/گرم اینترفرون بتا-1 دریافت نمودند و یا به مدت 4 هفته، به صورت 5 روز در هر هفته و روزانه 30 دقیقه، ورزش شنا داشتند. بافت مغز جدا و سطح عوامل پیش‌گفته به روش Enzyme-linked immunosorbent assay (ELISA) سنجیده شد. داده‌ها توسط آزمون One-way ANOVA تحلیل شدند.یافته‌ها: EAE منجر به کاهش BDNF و افزایش TrkB در مغز موش‌های مدل EAE شد. همچنین، نشان داده شد که شنا و تیمار اینترفرون بتا-1، منجر به افزایش این عوامل در مغز موش‌ها شد؛ در حالی که این افزایش به صورت آماری معنی‌دار نبود.نتیجه‌گیری: بر اساس یافته‌های این مطالعه، احتمال می‌رود ورزش نسبت به داروی اینترفرون بتا-1، عامل مؤثرتری در تغییر سطح BDNF و TrkB بافت مغز موش‌های مدل EAE ‌باشد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Four Weeks of Aerobic Swimming on the Levels of Brain-Derived Neurotrophic Factor (BDNF) and Tyrosine Receptor Kinase B (TrkB) in the Brain of Animal Model of Experimental Autoimmune Encephalitis (EAE)

نویسندگان [English]

  • Mehdi Seyed-Alhosseini 1
  • Hamid Rajabi 2
  • Ata Allah Ghadiri 3
  • Reza Gharakhanlou 4
  • Alireza Sarkaki 5
1 PhD Student, Department of Exercise Physiology, School of Physical Education and Sports Sciences, Kharazmi University, Tehran, Iran
2 Associate Professor, Department of Exercise Physiology, School of Physical Education and Sports Sciences, Kharazmi University, Tehran, Iran
3 Associate Professor, Cellular and Molecular Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4 Professor, Department of Physical Education, School of Humanities, Tarbiat Modares University, Tehran, Iran
5 Professor, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
چکیده [English]

Background: Multiple sclerosis (MS) is known as a neuroimmunological disease in human being. The evidences show that brain-derived neurotrophic factor (BDNF) and its specific receptor, tyrosine receptor kinase B (TrkB) play a role in the development of multiple sclerosis. Previous studies demonstrated that various interventions affect the expression of these factors. This study aimed to investigate the effect of four weeks of aerobic swimming on the level of BDNF and TrkB in the brain of mice with experimental autoimmune encephalomyelitis (EAE) or animal model of multiple sclerosis.Methods: A total number of 80 C57BL/6 mice, aging 10 to 12 weeks and weighing 20 ± 2 gram were divided into eight groups of 10, control, swimming (SW), EAE, EAE + SW, EAE + solvent (SOL), EAE + interferon-beta (IFN), EAE + environment (En) + SOL, and EAE + SW + IFN. On post-immunization day 9, animals received IFN (150 IU/g) or were subjected to swimming daily for 4 weeks (5 days/week). Brains were extracted and the levels of BDNF and TrkB were measured via enzyme-linked immunosorbent assay (ELISA). The data were analyzed using one-way ANOVA.Findings: EAE decreased BDNF and increased TrkB level in the brain of EAE-induced mice. Level of BDNF and TrkB increased in mice brain following swimming and IFN treatment; however these alterations were not significant.Conclusion: These findings suggest that probably swimming is more effective than IFN to alter the level of BDNF and TrkB in the brain of EAE-induced mice.

کلیدواژه‌ها [English]

  • Experimental autoimmune encephalomyelitis
  • Brain-derived neurotrophic factor
  • Tyrosine receptor kinase B (TrkB) receptor
  • Swimming

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مجله دانشکده پزشکی اصفهان
دوره 35، شماره 448 - شماره پیاپی 448
مهر و آبان 1396
صفحه 1263-1270

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سابقه مقاله

  • تاریخ دریافت: 23 مرداد 1396
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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