Changes of Serum Inflammatory Markers after Gamma-Secretase Inhibitor Administration Mice

Document Type : Original Article (s)

Authors

1 Associate professor, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Students Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

3 PhD Student, Department of Physiology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Notch signaling is involved in physiological and pathological processes and gamma-secretase inhibitors can suppress it. The aim of this study was to evaluate the effect of DAPT, a gamma secretase inhibitor, on serum inflammatory markers in mice.Methods: 24 C57BL6 mice (age: 5 weeks old) were randomly divided into control and obese groups. Obese group received high-fat diet (60% fat, 20% protein, and 20% carbohydrate) for 20 weeks. Control group received standard diet during this time. Then, each group was divided into receiving and non-receiving DAPT (50mg/kg; Subcutaneous). After 14 days, blood samples were taken for measurements of serum high-sensitive C-reactive protein (hs-CRP) and interlukin-6 (IL-6).Findings: There was no significant difference in serum hs-CRP concentration between control and obese groups (P > 0.05). Administration of DAPT did not alter serum hs-CRP level in both groups (P > 0.05). Serum Il-6 was significantly higher in obese animals compare to controls (P < 0.05) and administration of DAPT reduced serum IL-6 in control and obese animals (P < 0.05).Conclusion: DAPT, as a gamma secretase inhibitor, could affect several physiological and pathological vascular changes by alteration of serum inflammatory markers.

Keywords

References

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Journal of Isfahan Medical School
Volume 31, Issue 233 - Serial Number 233
March and April 2013
Pages 486-492

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History

  • Receive Date: 28 May 2013
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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