بررسی اثر تمرین هوازی، استرس صوتی و عصاره‌ی گل سفید ختمی بر تغییرات رفتارهای اضطرابی، سطح نیتریک اکساید و سروتونین پلاسما در موش‌های نر صحرایی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 استادیار، گروه تربیت بدنی، دانشکده‌ی علوم تربیتی و روانشناسی، دانشگاه سیستان و بلوچستان، زاهدان، ایران

2 دانشیار، گروه تربیت بدنی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه بوعلی سینا، همدان، ایران

3 استادیار، گروه تربیت بدنی و علوم ورزشی، دانشکده‌ی ادبیات و علوم انسانی، دانشگاه ملایر، ملایر، ایران

چکیده

مقدمه: استفاده از فعالیت‌های بدنی و داروهای گیاهی برای درمان بیماری‌های عصبی نظیر اضطراب سابقه‌ی طولانی دارد. مطالعه‌ی حاضر، با هدف بررسی اثر تمرین هوازی و تزریق عصاره‌ی گل سفید ختمی با استرس صوتی بر تغییرات رفتارهای اضطرابی، سطح نیتریک اکساید و سروتونین پلاسما در موش صحرایی نر انجام شد.روش‌ها: تعداد 35 سر موش صحرایی نر نژاد ویستار بالغ با محدوده‌ی وزنی 57/20 ± 82/170 گرم انتخاب شدند. موش‌ها به طور تصادفی به 5 گروه 7 تایی شامل شاهد، استرس صوتی، تمرین + استرس صوتی، عصاره‌ی گل سفید ختمی + استرس صوتی و استرس صوتی + تمرین + عصاره‌ی گل سفید ختمی تقسیم شدند. موش‌ها در معرض استرس صوتی صدای ضبط شده ترافیک 15 ± 95 دسی‌بل روزانه 5 ساعت به مدت 60 روز و تمرین هوازی بیشینه و زیر بیشینه به صورت 5 روز در هفته و به مدت 60 روز قرار گرفتند. تزریق عصاره‌ی گل سفید ختمی به میزان 500 میلی‌گرم به ازای هر کیلوگرم از وزن بدن داخل صفاقی صورت گرفت. برای تعیین غلظت نیتریک اکساید و سروتونین پلاسمای خون از کیت حیوانی مخصوص رت استفاده شد. جهت تجزیه و تحلیل نتایج از آزمون One-way ANOVA و آزمون تعقیبی Tukey استفاده و 05/0 ≥ P به عنوان سطح معنی‌داری در نظر گرفته شد.یافته‌ها: در گروه تزریق عصاره‌ی گل سفید ختمی + تمرین هوازی + استرس صوتی، میزان اضطراب کمتر و نیتریک اکساید و سروتونین زیادتر از گروه استرس صوتی در مقایسه با گروه شاهد بود (01/0 > P).نتیجه‌گیری: تمرین هوازی و تزریق عصاره‌ی ختمی میزان اضطراب و سروتونین پلاسمایی خون ناشی از استرس صوتی را جبران کرد؛ در این میان، نقش تمرین هوازی به مراتب بیشتر از عصاره‌ی گل سفید ختمی بود.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of Aerobic Training, Extract of Althaea kurdica and Noise Stress on the Anxiety-related Behaviors, Plasma Levels of Nitric Oxide and Serotonin in Wistar Male Rats

نویسندگان [English]

  • Amirhossien Karimi 1
  • Farzad Nazem 2
  • Akbar Sazvar 3
1 Assistant Professor, Department of Physical Education, School of Education and Psychology, University of Sistan and Bluchestan, Zahedan, Iran
2 Associate Professor, Department of Physical Education, School of Physical Education and Sport Science, Bu-Ali Sina University, Hamadan, Iran
3 Assistant Professor, Department of Physical Education and Sport Science, School of Literature and Humanities, Malayer University, Hamadan, Iran
چکیده [English]

Background: Physical activity and medicinal plants have been used years for the treatment of different neuropsychological diseases such as anxiety. This study aimed to assess the effects of training, Althaea kurdica extract injection, and noise stress on the anxiety-related behaviors, plasma levels of nitric oxide and serotonin in Wistar male rats.Methods: Thirty-five male rats were randomly divided into five groups of seven, noise, training + noise, extract + noise, training + noise + extract, and control groups. The aerobic training was performed 5 days per week, for a 60-day period. The noise stress included exposure to traffic noise 5 hours per day over a 60-day period (range: 95 ± 15 dB). The Althaea curdica extract (500 mg/kg of body weight) was injected intraperitoneally (IP) 30 minutes prior to the plus maze test. The control group was not exposed to any noise or exercise, and was kept away from the sources of stress; the rats were kept under the same conditions. At the end of the experiment, blood samples were collected and plasma nitric oxide (NO) and serotonin concentrations were determined with special kits for rat. For the statistical data analysis, ANOVA and Tukey post-hoc tests were used  at the significant level of P ≤ 0.05.Findings: In the noise + training + extract group, the anxiety was less and nitric oxide and serotonin were more than that of the noise stress group compared with the control group (P < 0.01).Conclusion: It seems that aerobic training and extract injections had eliminated the effect of noise stress, and apparently the effect of aerobic training was more significant than that of the extracts.

کلیدواژه‌ها [English]

  • Aerobic training
  • Noise stress
  • Althaea kurdica extract
  • Nitric oxide
  • Serotonin
  • Elevated plus maze test

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مجله دانشکده پزشکی اصفهان
دوره 33، شماره 357 - شماره پیاپی 357
آذر و دی 1394
صفحه 1843-1850

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سابقه مقاله

  • تاریخ دریافت: 07 مرداد 1393
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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