بررسی ارتباط بین پلی‌مورفیسم‌های ژنتیک IL33 و IL1RL1 و سطح سرمی IL-33 در بیماران مبتلا به آسم و Multiple Sclerosis

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه ایمنی‌شناسی، دانشکده‌ی پزشکی و کمیته‌ی تحقیقات دانشجویی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

2 استادیار، گروه ایمنی‌شناسی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

3 دانشیار، گروه ژنتیک و بیولوژی مولکولی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان،اصفهان، ایران

4 فوق تخصص آسم و آلرژی، کلینیک فوق تخصصی آسم و آلرژی اصفهان، اصفهان، ایران

چکیده

مقدمه: مطالعات اخیر نقش مهم محور IL-33/IL-1RL1 (Interleukin-33/Interleukin-1 receptor-like 1) را در بیماری‌های خودایمنی و التهابی نشان می‌دهد. هدف از این مطالعه، بررسی فراوانی و ارتباط پلی‌مورفیسم rs1342326 ژن IL-33 و پلی‌مورفیسم rs10204137 ژن IL-1RL1 و همچنین، سطح سرمی IL-33 در سه گروه افراد مبتلا به Multiple sclerosis (MS)، مبتلا به آسم و گروه شاهد بود.روش‌ها: از 140 بیمار مبتلا به آسم، 140 بیمار مبتلا به MS و 72 فرد سالم به عنوان گروه شاهد، نمونه‌گیری انجام شد. توزیع فراوانی آللی و ژنوتیپی دو پلی‌مورفیسم با استفاده از تکنیک HRM real-time PCR (High-resolution melting real-time polymerase chain reaction) مورد بررسی قرار گرفت. سطح سرمی IL-33 با استفاده از کیت ELISA (Enzyme-linked immunosorbent assay) (Boster) اندازه‌گیری شد.یافته‌ها: ارتباط معنی‌داری بین فراوانی پلی‌مورفیسم rs10204137 ژن IL-1RL1 و استعداد ابتلا به MS مشاهده شد (017/0 = P)؛ اما در افراد مبتلا به آسم، این ارتباط یافت نشد (093/0 = P). همچنین، ارتباط معنی‌داری بین فراوانی پلی‌مورفیسم rs1342326 ژن IL-33 در افراد مبتلا به آسم، مبتلایان به MS و نیز افراد سالم وجود نداشت (580/0 = P). سطح سرمی IL-33 در افراد مبتلا به MS و افراد مبتلا به آسم در مقایسه با افراد سالم مقادیر بالاتری را نشان داد (020/0 = P).نتیجه‌گیری: میزان سرمی IL-33 در دو گروه بیماران مبتلا به آسم و مبتلا به MS، افزایش قابل ملاحظه‌ای نسبت به افراد سالم داشت که نشان دهنده‌ی نقش این سایتوکاین در بیماری‌زایی این دو بیماری است.

کلیدواژه‌ها

عنوان مقاله [English]

The Relationship between the Genetic Polymorphisms of Interleukin-33 (IL-33) and Interleukin-1 Receptor-Like 1 (IL1R1) with the Levels of Interleukin-33 in Patients with Asthma and Multiple Sclerosis

نویسندگان [English]

  • Maryam Ahmadi 1
  • Nahid Eskandari 2
  • Fereshteh Ale-Sahebfosoul 2
  • Mansour Salehi 3
  • Ramin Ghasemi 4
1 MSc Student, Department of Immunology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
2 Assistant Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Associate Professor, Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Asthma and Allergy Specialist, Isfahan Asthma and Allergy Clinic, Isfahan, Iran
چکیده [English]

Background: Recent evidence suggests that the interleukin-33/interleukin-1 receptor-like 1 (IL-33/IL1RL1) axis plays a critical role in autoimmune and inflammatory disorders; however, its mechanistic role in these diseases has not been clearly defined. The present study aimed to investigate the frequency of rs1342326 polymorphism of IL-33 gene and rs10204137 polymorphism of IL1RL1 gene with serum levels of IL-33 in multiple sclerosis (MS), asthma and healthy subjects In Isfahan Province, Iran.Methods: Samples were taken from the patients with asthma (140) or multiple sclerosis (140) and healthy controls (72). Different genotypes of T/G or G/A polymorphisms were studied using the high-resolution melting real-time polymerase chain reaction (HRM real-time PCR) technique. Serum levels of IL-33 were measured via enzyme-linked immunosorbent assay (ELISA). Findings: There was not any relationship between the frequency of polymorphism rs10204137 of IL1RL1 gene and susceptibility to multiple sclerosis or asthma. There was no significant correlation between the frequency of rs1342326 polymorphism of IL-33 gene in healthy subjects compared to those with asthma or multiple sclerosis. Serum levels of IL-33 were higher than in patients with multiple sclerosis or asthma compared to healthy subjects.Conclusion: Serum levels of IL-33 in the two groups of patient increased substantially compared to the controls.

کلیدواژه‌ها [English]

  • Interleukin-33 (IL-33)
  • Multiple Sclerosis
  • Asthma
  • Polymorphism

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مجله دانشکده پزشکی اصفهان
دوره 33، شماره 361 - شماره پیاپی 361
بهمن و اسفند 1394
صفحه 2092-2101

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سابقه مقاله

  • تاریخ دریافت: 01 شهریور 1394
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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