بررسی تأثیر عصاره‌ی انار در بیان پروتئین کلاژن نوع II، نشانگر ویژه‌ی غضروف در روند کندروژنز سلول‌های بنیادی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه علوم تشریحی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

2 استاد، گروه فارماکوگنوزی، دانشکده‌ی داروسازی و علوم دارویی و مرکز تحقیقات علوم دارویی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

3 استادیار، گروه علوم تشریحی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

4 دانشیار، گروه علوم تشریحی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

مقدمه: بافت غضروف، فاقد عروق و اعصاب است و قابلیت ترمیم ندارد. در مهندسی بافت غضروف، از سلول‌های بنیادی و عوامل رشد استفاده می‌شود. در این مطالعه، تأثیر عصاره‌ی انار به عنوان عامل غضروف‌ساز سلول‌های بنیادی مشتق از بافت چربی بررسی گردید.روش‌ها: سلول‌های بنیادی مشتق از چربی انسانی، سومین پاساژ سلولی در محیط کشت القای کندروژنیک در داربست فیبرین به مدت 2 هفته تحت تأثیر عصاره‌ی انار کشت داده شدند. روش‌های (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) و Western blot برای بررسی سلول‌های تمایز یافته به کار رفت.یافته‌ها: سلول‌های بنیادی تحت تأثیر عصاره‌ی انار به کندروسیت تمایز یافتند و تولید کلاژن نوع II توسط سلول‌های متمایز شده به اثبات رسید.نتیجه‌گیری: عصاره‌ی انار، یک القا کننده‌ی مناسب جهت بیان پروتئین کلاژن نوع II در سلول‌های بنیادی مشتق از چربی است.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Pomegranate Extract on Producing Type II Collagen in Differentiation of Adipose-Derived Stem Cells into Chondrocytes

نویسندگان [English]

  • Mehri Katani 1
  • Behzad Zolfaghari 2
  • Mitra Soleimani 3
  • Ali Valiani 3
  • Batool Hashemibeni 4
1 MSc Student, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Professor, Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences AND Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
3 Assistant Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Associate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده [English]

Background: Cartilage tissue is avascular and has no repairing capacity. For cartilage tissue engineering, stem cells and growth factors are used. In this study, the effect of pomegranate as inducer for chondrogenesis of adipose-derived stem cells (ADSCs) was evaluated.Methods: Human adipose-derived stem cells in third passage seeded in fibrin were cultured in chondrogenic medium with pomegranate for 2 weeks. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay) and western blot technique were applied for evaluation of differentiated cells.Findings: Adipose-derived stem cells differentiated into chondrocytes; and type II collagen production by differentiated cells was proved.Conclusion: Pomegranate extract is an appropriate inducer for production of type II collagen in adipose-derived stem cells.

کلیدواژه‌ها [English]

  • Chondrogenesis
  • Stem cells
  • Pomegranate
  • Type II collagen

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مجله دانشکده پزشکی اصفهان
دوره 35، شماره 453 - شماره پیاپی 453
آذر و دی 1396
صفحه 1540-1545

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سابقه مقاله

  • تاریخ دریافت: 26 شهریور 1396
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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