اثر پیش درمان عصاره‌ی گل ازگیل ژاپنی بر سطوح BDNF (Brain-Derived neurotrophic factor)، SOD (Superoxide dismutase) و MDA (Malondialdehyde) در هیپوکامپ موش‌های در معرض 6 هیدروکسی دوپامین به دنبال 12 هفته تمرین اختیاری

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشیار، گروه فیزیولوژی ورزشی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

2 دانشجوی کارشناسی ارشد، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

چکیده

مقدمه: هدف از اجرای پژوهش، بررسی تأثیر عصاره‌ی هیدروالکلی گل ازگیل ژاپنی بر سطوح BDNF (Brain-derived neurotrophic factor)، SOD (Superoxide dismutase) و MDA (Malondialdehyde) در هیپوکامپ موش‌های پارکینسونی به دنبال 12 هفته تمرین اختیاری در چرخ دوار بود.روش‌ها: موش‌های صحرایی به 6 گروه شاهد سالم، شاهد پارکینسونی، تمرین، تمرین- سم، عصاره- سم و تمرین- عصاره- سم تقسیم شدند. گروه تمرین به مدت 12 هفته تمرین انجام داد. گروه تمرین- سم، 12 هفته تمرین انجام داد. سپس در معرض سم نرونی قرار گرفت. گروه تمرین- عصاره به مدت 12 هفته تمرین کرد و هر هفته سه بار عصاره را به صورت صفاقی و به میزان mg/kg 200 دریافت کرد. تخریب هیپوکامپ با تزریق استریوتاکسی محلول 6-هیدروکسی دوپامین به داخل بطن مغز صورت گرفت. داده‌ها به روش One way analysis of variance (One way ANOVA) و آزمون تعقیبی LSD (Fisher's least significant difference) تجزیه و تحلیل شد.یافته‌ها: تمرین، BDNF را افزایش داد؛ اما تمرین و عصاره به تنهایی نتوانستند از کاهش BDNF در اثر سم جلوگیری کنند و تفاوت بین گروه‌های شاهد سالم و گروه تمرین- عصاره معنی‌دار بود (001/0 = P). تمرین به طور معنی‌داری موجب افزایش SOD در مقایسه با گروه شاهد شد (001/0 = P)؛ اما نتوانست در مقابل آثار سم جلوگیری کند (001/0 = P). تفاوت سطوح SOD بین گروه عصاره- تمرین با گروه شاهد معنی‌دار نبود (125/ = P). تمرین اثر پیشگیرانه در برابر افزایش MDA نداشت (001/0 = P). تفاوت MDA بین گروه عصاره‌ی پارکینسون و شاهد سالم معنی‌دار نبود (904/0 = P). همچنین اختلاف سطح MDA بین دوگروه شاهد سالم و گروه تمرین و عصاره معنی‌دار نبود (918/0 = P).نتیجه‌گیری: ورزش اختیاری همراه با مصرف عصاره‌ی ازگیل سبب افزایش ظرفیت آنتی اکسیدانی هیپوکمپ می‌شود، اما نمی‌تواند مانع از کاهش معنی‌دار BDNF شود. احتمال دارد با افزایش SOD و کاهش MDA، دیگر نیازی به بالا رفتن سطح BDNF برای مقابله با استرس اکسایشی تولید شده به دنبال ورزش نباشد.

کلیدواژه‌ها

عنوان مقاله [English]

Pre-treatment Effects of Eriobotrya Japonica Extraction on Malondialdehyde (MDA), Brain-Derived Neurotrophic Factor (BDNF), and Superoxide Dismutase (SOD) Levels in Hippocampus of Parkinsonian Rats Induced by 6-hydroxydopamine Following 12 weeks of Volunt

نویسندگان [English]

  • Zia Fallah Mohammadi 1
  • Razieh Mohammadi 2
  • Jalil Aslani 2
1 Associate Professor, Department of Exercise Physiology, School of Physical Education and Sports Sciences, University of Mazandaran, Babolsar, Iran
2 MSc Student, School of Physical Education and Sports Sciences, University of Mazandaran, Babolsar, Iran
چکیده [English]

Background: The purpose of this study was to investigate the effects of extract of Eriobotrya japonica flower on brain-derived neurotrophic factor (BDNF), superoxide dismutase (SOD), and malondialdehyde (MDA) levels in the hippocampus of Parkinsonian rats after 12 weeks of voluntary exercise on a running wheel.Methods: 43 rats were divided into six groups of healthy control, Parkinsonian control, training group, Parkinsonian training, extract Parkinsonian, and training-extract Parkinsonian. Training group exercised on running wheels for 12 weeks. Training-extract group exercised on running wheels and received 200 mg/kg extract of Eriobotrya japonica intraperitoneally three times per week during study period. To induce Parkinson, 6-hydroxydopamine (6-OHDA) (dissolved in saline) was administered intracerebrovenricular (ICV) by a stereotaxic apparatus. BDNF level were measured using the enzyme-linked immunosorbent assay (ELISA) test. SOD and MDA levels were measured using spectrophotometric method. Data was analyzed using one-way analysis of variance (ANOVA) and least significant difference (LSD) post-hoc tests.Findings: Exercise in Parkinsonian-training and extract in Parkinsonian-extract group could not prevent decrease of BDNF and difference between healthy control and training-extract group was significant (P = 0.001). Voluntary wheel running and extract significantly prevented SOD level to decrease in Parkinsonian rats (P = 0.001). Differences of SOD level between training-extract and healthy group were not significant (P = 0.125). Training and extraction could not prevent MDA level to increase compared with healthy control group (P = 0.918). The difference of MDA level between extract-Parkinsonian and healthy group was not significant (P = 0.904).Conclusion: Pre-treatment with voluntary exercise and extraction of Eriobotrya japonica increase oxidative protection capacity of hippocampus against 6-OHDA toxicity but they cannot prevent significant decrease of BDNF level against 6-OHDA. Possibly, along with increase of SOD and decrease of MDA there would be no need to BDNF increase in order to counteract oxidative stress induced by exercise.

کلیدواژه‌ها [English]

  • Eriobotrya japonica
  • Voluntary exercise
  • 6-hydroxydopamine

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مجله دانشکده پزشکی اصفهان
دوره 32، شماره 274 - شماره پیاپی 274
فروردین و اردیبهشت 1393
صفحه 120-130

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سابقه مقاله

  • تاریخ دریافت: 05 مرداد 1392
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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