اثر تمرین اینتروال متوسط شدت و لیپوئیک اسید لیپوزومال بر لنف آنژیوژنز پانکراس، گلوکز و انسولین سرمی Rat‌های مدل مبتلا به دیابت

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 استادیار، گروه تربیت بدنی، واحد آزادشهر، دانشگاه آزاد اسلامی، آزادشهر، ایران

2 گروه تربیت بدنی، واحد آزادشهر، دانشگاه آزاد اسلامی، آزادشهر، ایران

3 استادیار ،گروه تربیت بدنی، واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران

چکیده

مقدمه: لنف آنژیوژنز، نقش مهمی در التهاب جزایر و تنظیم انسولین دارد. این پژوهش، با هدف بررسی اثر تمرین اینتروال متوسط شدت و مکمل آلفالیپوئیک اسید بر Vascular endothelial growth factor receptor 3 (VEGFR3) بافت پانکراس، گلوکز و انسولین سرمی Rat‌های نر نژاد Wistar مدل مبتلا به دیابت انجام شد.روش‌ها: در یک مطالعه‌ی تجربی، 25 سر Rat نر نژاد Wistar، با میانگین سن 3 هفته و وزن 3 ± 159 گرم به پنج گروه (5 = n در هر گروه) شامل گروه شاهد سالم، دیابت + سالین، دیابت + مکمل، دیابت + تمرین + سالین، دیابت + مکمل + تمرین تقسیم شدند. القای دیابت، با تزریق داخل صفاقی استرپتوزوتوسین (65 میلی‌گرم/کیلوگرم) و نیکوتین امید (120 میلی‌گرم/کیلوگرم) انجام شد. آلفالیپوئیک اسید (Alpha lipoic acid یا ALA) به مدت 6 هفته، با دز 20 میلی‌گرم/کیلوگرم روزانه به صورت خوراکی تجویز شد. تمرین اینتروال، به مدت6 هفته، 5 جلسه در هفته شامل 13 تناوب 4 دقیقه‌ای دویدن روی تردمیل با شدت VO2max معادل 70-65 درصد و استراحت فعال بین تناوب‌ها، 2 دقیقه دویدن با شدت 10-5 متر/دقیقه بود. پس از کشتار Ratها و بافت‌برداری، بیان پروتئین VEGFR3 بافت پانکراس به روش ایمنوهیستوشیمی و گلوکز به روش کالری‌متریک و انسولین به روش enzyme-linked immunoassay (ELISA)-کالری‌متریک انجام شد. داده‌ها با آزمون One-way ANOVA و با استفاده از نرم‌افزار SPSS در سطح معنی‌داری 050/0 > P تجزیه و تحلیل شدند.یافته‌ها: 6 هفته تمرین اینتروال و تمرین اینتروال با مکمل Lysosomal acid lipase (LAL) بر VEGFR3 بافت پانکراس، گلوکز و انسولین سرمی Rat‌های مبتلا به دیابت تأثیر معنی‌داری داشت. مصرف 6 هفته مکمل LAL بر VEGFR3و انسولین سرمی Ratهای مبتلا به دیابت اثر معنی‌داری داشت.نتیجه‌گیری: تمرین اینتروال شدید و مکمل ALA باعث افزایش لنف آنژیوژنز و کاهش گلوکز خون و تغییرات مطلوب در رت‌های دیابتی شد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Moderate-Intensity Interval Training (MIIT) and Liposomal Lipoic Acid on Lymphangiogenesis, Glucose, and Serum Insulin in Diabetic Rats

نویسندگان [English]

  • Minoo Dadban-Shahamat 1
  • Seyed-Ramin Hashemian-Esfahani 2
  • Asra Askari 3
1 Assistant Professor, Department of Physical Education, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran
2 Department of Physical Education, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran
3 Assistant Professor, Department of Physical Education, Gorgan Branch, Islamic Azad University, Gorgan, Iran
چکیده [English]

Background: Lymphangiogenesis plays an important role in islet inflammation and insulin regulation. In this study, the effect of interval training and liposomal alpha lipoic acid supplementation on serum glucose, blood insulin, and vascular endothelial growth factor receptor-3 (VEGFR3) was examined in diabetic rat.Methods: 25 male Wistar rats with an average age of 3 weeks, and weighing 159  ±3 g were divided in seven groups (n = 5) of control (healthy), diabetes + saline, diabetes + supplement, diabetes + exercise + saline, and diabetes + supplement + exercise. To induce diabetes, streptozotocin (65 mg/kg) was injected subcutaneously, and then nicotine amid (120 mg/kg) was injected. Alpha lipoic acid was administered orally (20 mg/kg/day) for 6 weeks. The training program included intermediate interval training, including 6 weeks of training, 5 session per week, consisted of 13 periods of 4 minutes with an intensity of 70-65 percent VO2max running on a treadmill. Active rest between intervals was in the form of 2-minute runs with an intensity of 5-10 m/minute. After killing rats and sampling, pancreatic tissue was sent to the laboratory to examination the expression of VEGFR3 protein by immunohistochemistry, glucose by calorimetric method, and insulin by enzyme-linked immunoassay (ELISA)-calorimetric method. Data were analyzed using one-way ANOVA in SPSS software at a significant level of P < 0.050.Findings: 6 weeks of interval training and interval training with liposomal alpha lipoic acid had a significant effect on VEGFR3 pancreatic tissue, glucose, and serum insulin in diabetic rats. 6 weeks of liposomal alpha lipoic acid supplementation had a significant effect on VEGFR3 and serum insulin in diabetic rats.Conclusion: Intense interval training and liposomal alpha lipoic acid supplementation increased lymphangiogenesis and decreased blood glucose and favorable changes in diabetic rats.

کلیدواژه‌ها [English]

  • Diabetes Mellitus
  • Lipoic acid
  • Exercise
  • Vascular endothelial growth factor receptor-3
  • Blood glucose

مراجع

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سابقه مقاله

  • تاریخ دریافت: 19 اردیبهشت 1400
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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