The Effect of Aerobic Training and Pistacia Atlantica Extract on the Levels of Heat Shock Protein 70 and Protein Carbonyl in the Heart Tissue of Diabetic Rats

Document Type : Original Article (s)

Authors

1 Department of Physical Education, School of Physical Education and Sport Sciences, University of Birjand, Birjand, Iran

2 Associate Professor, Department of Physical Education, School of Physical Education and Sport Sciences, University of Birjand, Birjand, Iran

3 Assistant Professor, Atherosclerosis and Coronary Heart Research Center, Birjand University of Medical Sciences, Birjand, Iran

4 PhD Student, Department of Physical Education, School of Physical Education and Sport Sciences, University of Birjand, Birjand, Iran

Abstract

Background: Using the combination of exercise training and medicine, especially herbal medicine, is one of the therapeutic approaches in patients with diabetes. Purpose of the present study was to assess the effect of a 6-week aerobic training together with consuming of Pistacia atlantica extract on heat shock protein 70 (HSP70) and protein carbonyl (PC) levels in the heart tissue of streptozotocin-induced diabetic rats.Methods: In this experimental study, 40 male Wistar rats were randomly divided into five groups. Diabetes was induced via intraperitoneal injection of streptozotocin (40 mg/kg). The six-week period exercise training program included aerobic training on a treadmill (five sessions per week, 40 minutes per session, with a speed of 20 m/minute and with a slope of 5 degrees). Pistacia atlantica extract was fed five days per week with a dose of 25 mg/kg. The rats were anesthetized 48 hours after the last training session, and their heart were isolated. Then, the levels of HSP70 and protein carbonyl in the heart tissue were determined using enzyme-linked immunoassay (ELISA) and chemical colorimetric method, respectively.Findings: The HSP70 level in the diabetic group under aerobic training together with receiving Pistacia atlantica extract was significantly lower than the control diabetic group (P = 0.003); but, there was no statistically significant difference in the level of protein carbonyl between the groups (P = 0.740).Conclusion: It seems that aerobic training with Pistacia atlantica extract consuming, is a better therapeutic approach than using them alone, as a complementary therapy for reducing cardiac side effects in patients with diabetes.

Keywords

References

  1. Seghrouchni I, Drai J, Bannier E, Riviere J, Calmard P, Garcia I, et al. Oxidative stress parameters in type I, type II and insulin-treated type 2 diabetes mellitus; insulin treatment efficiency. Clin Chim Acta 2002; 321(1-2): 89-96.
  2. Amouoghli-Tabrizi B, Mohajeri D. Protective effect of turnip root ethanolic extract on early diabetic nephropathy in the rats. Zahedan J Res Med Sci 2011; 13(6): 13-9. [In Persian].
  3. Sadi G, Kartal DI, Guray T. Regulation of glutathione S-transferase Mu with type 1 diabetes and its regulation with antioxidants. Turk Biyokimya Dergisi 2013; 38(1): 92-100.
  4. Dennis KE, Hill S, Rose KL, Sampson UK, Hill MF. Augmented cardiac formation of oxidatively-induced carbonylated proteins accompanies the increased functional severity of post-myocardial infarction heart failure in the setting of type 1 diabetes mellitus. Cardiovasc Pathol 2013; 22(6): 473-80.
  5. Rezaei Avval M, Esteghamati A, Alamdari A, Morteza A, Meisami AP, Nakhjavani M. HSP70 and type 2 diabetes. Iran J Diabetes Lipid Disord 2010; 9(3): 276-82. [In Persian].
  6. Farzanegi P, Mousavi M, Ghanbari-Niaki A. Effect of Pistacia atlantica extract on glutathione peroxidase tissue levels and total oxidative capacity of liver and plasma lipid profile of rats. Zahedan J Res Med Sci 2013; 15(11): 59-63. [In Persian].
  7. Isanejad A, Hasan-Sarraf Z, Mahdavi M, Gharakhanlou R. The effect of aerobic exercise training on serum levels of TNF-α, IL-1β, IL-6 and Hsp70 in rats. Journal of Sport Biosciences 2013; 4(15): 91-106. [In Persian].
  8. Hashemi A, Faramarzi M, Bargharar M, Khazani A, Amani S, Banitalebi E. A comparison of the effects of Carbohydrate and Carbohydrate-Protein supplements on heat shock protein 72(HSP72) during intermittent soccer activities. Iran J Nutr Sci Food Technol 2012; 7(3): 79-88. [In Persian].
  9. Mahmudzadeh T, Saghebjoo M, Seghatol Eslami A, Hedayati M. Effect of aerobic training and Pistacia atlantica extract consumption on pancreatic a-cells function in streptozotocin-induced diabetic rats. Iran J Diabetes Lipid Disord 2014; 13(3): 252-62. [In Persian].
  10. Zarekar M, Saghebjoo M, Foadodini M, Hedayati M. Combined effect of aerobic training and Pistacia athlantica extract on GLUT-4 protein expression and muscle glycogen in diabetic rats. Iran J Endocrinol Metab 2014; 16(4): 245-53.
  11. Mohammadi Karizno F, Saghebjoo M, Foadoddini M, Sarir H. The role of aerobic training and Pistacia atlantica extract on the levels of protein carbonyl, heat shock protein 70, and glycogen in the liver tissue of diabetic rats. J Birjand Univ Med Sci 2014; 21(1): 35-47. [In Persian].
  12. Bloomer RJ, Goldfarb AH, McKenzie MJ. Oxidative stress response to aerobic exercise: comparison of antioxidant supplements. Med Sci Sports Exerc 2006; 38(6): 1098-105.
  13. Senturk UK, Gunduz F, Kuru O, Kocer G, Ozkaya YG, Yesilkaya A, et al. Exercise-induced oxidative stress leads hemolysis in sedentary but not trained humans. J Appl Physiol (1985) 2005; 99(4): 1434-41.
  14. Chis IC, Baltaru D, Maier M, Muresan A, Clichici S. ffects of quercetin and chronic (training) exercise on oxidative stress status in animals with streptozotocin-induced diabetes. Bulletin UASVM, Veterinary Medicine 2013; 70(1): 31-9.
  15. Tian HL, Wei LS, Xu ZX, Zhao RT, Jin DL, Gao JS. Correlations between blood glucose level and diabetes signs in streptozotocin-induced diabetic mice. Global J Pharmacol 2010; 4(3): 111-6.
  16. Chrystall BB, Devine CE. Electrical stimulation of rats: A model for evaluating low voltage stimulation parameters. Meat Sci 1983; 9(1): 33-41.
  17. Weitgasser R, Davalli AM, Weir GC. Measurement of glucose concentrations in rats: differences between glucose meter and plasma laboratory results. Diabetologia 1999; 42(2): 256.
  18. Tatsuki R, Satoh K, Yamamoto A, Hoshi K, Ichihara K. Lipid peroxidation in the pancreas and other organs in streptozotocin diabetic rats. Jpn J Pharmacol 1997; 75(3): 267-73.
  19. Liu J, Yeo HC, Overvik-Douki E, Hagen T, Doniger SJ, Chyu DW, et al. Chronically and acutely exercised rats: biomarkers of oxidative stress and endogenous antioxidants. J Appl Physiol (1985) 2000; 89(1): 21-8.
  20. Cai L, Wang Y, Zhou G, Chen T, Song Y, Li X, et al. Attenuation by metallothionein of early cardiac cell death via suppression of mitochondrial oxidative stress results in a prevention of diabetic cardiomyopathy. J Am Coll Cardiol 2006; 48(8): 1688-97.
  21. Suji G, Sivakami S. Approaches to the treatement of diabetes mellitus: an overview. Cell Mol Biol (Noisy -le -grand) 2003; 49(4): 635-9.
  22. Shirpoor A, Salami S, Khadem Ansari M, Ghaderi Pakdel F, Khadem Vatani K, Saadatian R, et al. Protective effect of vitamin E on diabetes induced apoptosis and oxidative stress in rat heart tissue. Iran J Endocrinol Metab 2008; 10(1): 67-74. [In Persian].
  23. Cheeseman KH, Slater TF. An introduction to free radical biochemistry. Br Med Bull 1993; 49(3): 481-93.
  24. Simar D, Malatesta D, Mas E, Delage M, Caillaud C. Effect of an 8-weeks aerobic training program in elderly on oxidative stress and HSP72 expression in leukocytes during antioxidant supplementation. J Nutr Health Aging 2012; 16(2): 155-61.
  25. Nakhjavani M, Morteza A, Khajeali L, Esteghamati A, Khalilzadeh O, Asgarani F, et al. Increased serum HSP70 levels are associated with the duration of diabetes. Cell Stress Chaperones 2010; 15(6): 959-64.
  26. Atalay M, Oksala NK, Laaksonen DE, Khanna S, Nakao C, Lappalainen J, et al. Exercise training modulates heat shock protein response in diabetic rats. J Appl Physiol (1985) 2004; 97(2): 605-11.
Journal of Isfahan Medical School
Volume 33, Issue 347 - Serial Number 347
July and August 2015
Pages 1337-1348

Files

History

  • Receive Date: 21 December 2014
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

Share

How to cite

Statistics