Genotyping of Salmonella Enterica Serovar Enteritidis Strains Isolated from Clinical Samples by Pulsed-Field Gel Electrophoresis (PFGE)

Document Type : Original Article (s)

Authors

1 Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

2 Associate Professor, Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Abstract

Background: The infections caused by Salmonella enterica serovar enteritidis (Salmonella enteritidis) have been increasing in developed and developing countries. The aim of this study was to investigate the genetic relationship between the strains of Salmonella enteritidis.Methods: In a descriptive study from 2008 to 2010, clinical Salmonella enteritidis strains were collected from the patients referred to different hospitals in Tehran. The strains identified by biochemical and serological methods. Genetic relationships between the isolates were investigated using pulsed field gel electrophoresis (PFGE) method.Findings: Of 40 Salmonella enteritidis isolated strains, two PFGE profiles were obtained which among them, 32 (80%) and 8 (20%) of isolates belonged to cluster A and cluster B, respectively. Based on PFGE patterns, 28 (70%) strains exhibited nine bands and were belonged to cluster A.Conclusion: The result of this study indicated that isolated Salmonella enteritidis strains belonged to the closed clones.

Keywords

References

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Journal of Isfahan Medical School
Volume 31, Issue 240 - Serial Number 240
May and June 2013
Pages 819-829

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History

  • Receive Date: 28 January 2013
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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