Document Type : Original Article(s)
Authors
1
Assistant Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
2
Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
3
Associate Professor, School of Chemistry, The University of Isfahan, Isfahan, Iran.
4
MSc Student, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
5
Associate Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
6
Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
7
PhD Student, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Abstract
Background: Anatomists had been searching to find methods for preserving soft human tissues and produce natural samples with durable, dry, odorless, and portable in briefcase properties. Plastination is a modern method for preparing such tissue samples. In plastination, water and fat are extracted from tissues and replaced with a dissolver fluid, which in its turn is replaced with a special kind of polyester. Usually, the organs like hearts, lungs, kidneys, striated muscles, vessels, nerves, connective tissue, etc, which are treated with formalin, loss their beautiful color and take a dull appearance after fixation. Tissue staining with natural dyes is one method for somehow preserving tissue natural color and increasing its appearance activity to prepare better samples in terms of appearance and suitable clearance of different parts (valves, muscles, trabeculae…). Also, we have to prepare non fragile samples. Methods: At first, some visceral organs (sheep hearts) were prepared and fixed by formalin 5% for 4 to 10 days. Then they were dissected for anatomic details and were put in a chemical dye called eosin and natural dye called ronnas. After dehydration by acetone in -25 °C and defeating with acetone in +30 °C, samples were put in the vacuum chamber to replace acetone instead of polymer under the vacuum pressure; finally the samples were cured with the ultra violate wave. Findings: We found that after fixation and before plastination staining of organs with eosin and ronnas resulted in beautiful appearance of tissues. Conclusion: Staining of organs resulted in beautiful appearance of tissues which are effective in increasing the attractively of teaching and learning anatomy. Also, it has affection in the raising appearance quality and has positive impression in the quality of education. Keywords: Plastination, Natural dye, Industrial dye, Polyester.
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