اثر تمرین هوازی و امگا-3 بر عوامل التهابی و استرس اکسیداتیو بافت قلب موش‌های سالمند تغذیه شده با رژیم غذایی پرچرب

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران

2 دانشیار، گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران

چکیده

مقاله پژوهشی




مقدمه: افزایش سن و چاقی با التهاب سیستمیک و افزایش استرس اکسیداتیو همراه است. فعالیت ورزشی هوازی و امگا-3 نقش مهمی در تعدیل استرس اکسیداتیو و التهاب دارند. هدف از پژوهش حاضر، بررسی اثر تمرین هوازی و امگا-3 بر عوامل التهابی و استرس اکسیداتیو بافت قلب موش‌های سالمند تغذیه شده با رژیم غذایی پرچرب می‌باشد.
روش‌ها: در این مطالعه‌ی تجربی، 40 سر موش صحرایی نر نژاد ویستار (میانگین وزن 26/95 ± 311/32) در پنج گروه رژیم غذایی نرمال (Normal diet) ND، رژیم غذایی پرچرب (High-fat diet) HFD، رژیم غذایی پرچرب-تمرین (High-fat diet-training ) HFDT، رژیم غذایی پرچرب- امگا-3 (High-fat diet-omega3) HFDω3، تمرین-رژیم غذایی پرچرب-امگا-3 (High-fat diet-training-omega3) HFDTω3 قرار گرفتند. گروه‌های مکمل، طی دوره‌ی مداخله، روزانه 1 ‌گرم امگا-3 (به ازای هر کیلوگرم وزن بدن) را به صورت خوراکی دریافت کردند. برنامه‌ی تمرین هوازی شامل دویدن روی تردمیل با شدت 60-50 درصد حداکثر اکسیژن مصرفی (VO2max)، پنج روز هفته به مدت هشت هفته اجرا شد. غلظت بافتی گلوتاتیون پراکسیداز (Glutathione peroxidase) GPX، کاتالاز (Catalase) CAT، سوپراکسید دیسموتاز (Superoxide dismutase) SOD و مالون‌دی‌آلدئید (Malondialdehyde) MDA به روش الایزا با استفاده از کیت‌های مخصوص موش‌های صحرایی، اندازه‌گیری شد.
یافته‌ها: بیان ژن TNF-α، IL-6 و NF-κB در گروه‌های HFDT، HFDω3 و HFDTω3، کاهش معنی‌داری داشت. این کاهش در گروه‌های HFDTω3 نسبت به HFDT و HFDω3 نیز مشاهده شد. همچنین کاهش معنی‌داری در مقادیر MDA مشاهده گردید. افزایش معنی‌دار در مقادیر Nuclear factor erythroid 2–related factor 2 = Nrf2، SOD، GPX و کاتالاز در گروه‌های تجربی، نسبت به گروه HFD مشاهده شد.
نتیجه‌گیری: تمرین هوازی همراه با امگا-3 ممکن است با کاهش التهاب و فشار اکسایش، باعث بهبود آسیب قلبی ناشی از HFD در موش‌های سالمند شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Aerobic Exercise and Omega-3 on Inflammatory and Oxidative Stress in the Heart Tissue of Elderly HFD Rats

نویسندگان [English]

  • Ghasem Torabi Palat Kaleh 1
  • Ahmad Abdi 2
  • Asieh Abbasi-Daloii 2
1 PhD Candidate, Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2 Associate Professor, Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
چکیده [English]

Background: Aging and obesity are associated with systemic inflammation and increased oxidative stress. Aerobic exercise and omega-3 play an important role in modulating oxidative stress and inflammation. The purpose of this study is to investigate the effect of aerobic exercise and omega-3 on inflammatory and oxidative stress in the heart tissue of elderly rats fed a high-fat diet.
Methods: In this experimental study, 40 male Wistar rats (mean weight 311.32 ± 26.95) were divided into
5 groups: Normal Diet (ND), High-Fat Diet (HFD), High-Fat Diet-Training (HFDT), High-Fat Diet-Omega3 (HFDω3) and High-Fat Diet-Training-Omega3 (HFDTω3). The supplement groups received 1 g of Omega3 (per kg of body weight) orally during the intervention period.An aerobic exercise program including running on a treadmill with an intensity of 50-60% maximal oxygen consumption (VO2max), was performed 5 days a week for eight weeks. The tissue concentration of glutathione peroxidase (GPX), catalase (CAT), superoxide dismutase (SOD) and malondialdehyde (MDA) was measured by ELISA method using special kits for rats.
Findings: Significant decrease were seen in TNF-α, IL-6, and NF-κB gene expression levels in HFDT, HFDω3, and HFDTω3. This decrease was also observed in the HFDTω3 group compared with the HFDT group and HFDω3 group. Also, significant decrease was observed in MDA values. Findings showed significant increase in Nuclear factor erythroid 2-related factor 2, SOD, GPX, and CAT in experimental groups compared with HFD.
Conclusion: Combining aerobic exercise with omega-3 may ameliorate HFD-induced heart damage in elderly rats by reducing inflammation and oxidative stress.

کلیدواژه‌ها [English]

  • Exercise
  • Omega-3 fatty acid
  • Inflammation
  • Oxidative stress
  • Aging and obesity

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مجله دانشکده پزشکی اصفهان
دوره 41، شماره 716
هفته 4 خرداد
خرداد و تیر 1402
صفحه 277-286

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سابقه مقاله

  • تاریخ دریافت: 01 اسفند 1401
  • تاریخ پذیرش: 16 اردیبهشت 1402

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