نوع مقاله : مقاله های پژوهشی
نویسندگان
1 دانشجوی کارشناسی ارشد، گروه علوم تشریحی، دانشکدهی پزشکی و کمیتهی تحقیقات دانشجویی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
2 دانشیار، گروه علوم تشریحی، دانشکدهی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
3 استادیار، گروه علوم تشریحی، دانشکدهی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
4 دانشجوی دکتری، گروه ایمنی شناسی، دانشکدهی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
5 گروه علوم تشریحی، دانشکدهی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Background: Due to increased prevalence of cancer in human population, the effectiveness of chemotherapy and drug (therapy) is reducing because of recurrence and drug-resistance. Therefore, scientists are trying other options such as cell-therapy. Among the cells, scientists have more focused on evaluating effect of stem cells on tumor cells. Since there were controversial reports on this effect in literature, the aim of this study was to assess the proliferation and survival of K562 tumor cells cocultured with stem cells from human exfoliated deciduous teeth (SHED).Methods: Stem cells were isolated from human exfoliated deciduous teeth and cultured K562 cells were obtained from the Pasteur institute of Iran. Stem cells and K562 were directly cocultured with each other and the ratios of K562 to stem cells were 1/10, 10/1, and 1/1. In this study, the viability and proliferation of the cells via MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and trypan blue staining.Findings: The trypan blue and MTT assay results of 48 hours coculture with ratio of 1/10 in comparison with control group did not show any significance difference. But with this ratio, at 96 hour, proliferation of K562 compared to control had reduced significantly; and in other ratios (1/1 and 1/10) at 48 and 96 hours, proliferation of K562 increased significantly (P < 0.05).Conclusion: Results of our study revealed that in this coculture, increased number of stem cells had an inhibitory effect on proliferation of K562 cells. Moreover, low numbers of stem cells not only were not able to inhibit tumor cells, but also contributed to improve their proliferation.
کلیدواژهها [English]