تأثیر میدان مغناطیسی کم فرکانس بر سرعت رشد و تکثیر سلول‌های بنیادی مزانشیمی مشتق از بافت چربی انسانی

نوع مقاله : Original Article(s)

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه فیزیک و مهندسی پزشکی، دانشکده‌ی پزشکی و کمیته‌ی تحقیقات دانشجویی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

2 استاد، گروه فیزیک و مهندسی پزشکی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

3 دانشیار، گروه فیزیک و مهندسی پزشکی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

4 دانشیار، گروه علوم تشریح و بیولوژی مولکولی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

مقدمه: سلول‌های بنیادی به دلیل قابلیت تکثیر و تمایز به سلول‌های دیگر مدل خوبی برای بررسی اثرات میدان مغناطیسی کم فرکانس (Extremely low-frequency electromagnetic field یا ELF-EMF) بر سلول‌های بدن می‌باشند. بافت چربی به عنوان منبعی از سلول‌های بنیادی مزانشیمی شناخته می‌شود. در این مطالعه تأثیر تابش با شدت‌های مختلف بر میزان تکثیر بر سلول‌های بنیادی مزانشیمی مشتق‌شده از بافت چربی انسانی در زمان‌های تابش20 و 40 دقیقه بررسی شد.روش‌ها: در این مطالعه تأثیر تابش با شدت 5/0 و 1 میلی‌تسلا و فرکانس 50 هرتز بر میزان تکثیر سلول‌های بنیادی مزانشیمی مشتق‌شده از بافت چربی انسانی با زمان‌های تابش20 و 40 دقیقه در روز به مدت 7 روز متوالی بررسی گردید. از تکنیک MTT assay برای بررسی رشد و میزان بقای سلول‌ها و از رنگ‌آمیزی تریپان بلو به منظور ارزیابی میزان تکثیر سلول‌ها استفاده گردید. آنالیز داده‌ها با استفاده از آزمون One way ANOVA انجام شد.یافته‌ها: میزان تکثیر و بقای سلول‌ها در همه‌ی گروه‌های تابش به طور معنی‌داری بیشتر از گروه‌های شاهد بود (05/0 > P) و تنها گروه 1 میلی‌تسلا به مدت 40 دقیقه در روز، اختلاف معنی‌داری با گروه شاهد نداشت.نتیجه‌گیری: نتایج مطالعه‌ی حاضر نشان داد که تابش میدان مغناطیسی با شدت‌های 5/0 و 1 میلی‌تسلا با توجه به زمان تابش می‌تواند باعث تحریک رشد و تکثیر سلول‌ها شود. مکانیسم این تأثیر هنوز ناشناخته می‌باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Extremely Low-Frequency (50 Hz) Field on Proliferation Rate of Human Adipose-Derived Mesenchymal Stem Cells

نویسندگان [English]

  • Marzieh Salimi 1
  • Daryoush Shahbazi-Gahrouei 2
  • Saeid Karbasi 3
  • Saied Kermani 3
  • Shahnaz Razavi 4
1 MSc Student, Department of Medical Physics and Medical Engineering, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
2 Professor, Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Associate Professor, Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Associate Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده [English]

Background: The effects of non-ionizing extremely low-frequency electromagnetic fields (ELF-EMF) chronic exposure on human beings due to its potential health hazards has become a focus of interest since many years ago. Stem cells are useful models for assessment of effects of ELF-EMF on other cell lines and human beings. Adipose tissue has been known source of multipotent stromal cells (MSCs), which can be obtained by a less invasive method and in large amounts compared with bone marrow stromal cells (BMSCs); so this study was done on human adipose-derived stem cells (hADSCs). The effect of ELF-EMF with intensity of 0.5 and 1 mT and 50 Hz on proliferation rates of hADSCs at 20 and 40 min/day for 7 days was assessed.Methods: MTT assay was used to determine the growth and metabolism of cells and Trypan blue test was also done for cell viability.Findings: The proliferation rate and growth of hADSCs in all exposure groups was significantly higher than that in sham groups except in group of 1 mT, 40 min/day (P < 0.05).Conclusion: The results showed that 0.5 and 1 mT magnetic strength fields can promote the proliferation rates of the hMSCs derived adipose tissue regarding the duration of exposure.

کلیدواژه‌ها [English]

  • Non-ionizing extremely low-frequency electromagnetic fields (ELF-EMF)
  • Human adipose-derived stem cells (hADSCs)
  • Adipose
  • Proliferation rate
  • Growth

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مجله دانشکده پزشکی اصفهان
دوره 31، شماره 232 - شماره پیاپی 232
خرداد و تیر 1392
صفحه 439-455

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  • تاریخ دریافت: 28 فروردین 1392
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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