The Aloe Vera Gel Improves Structural Disorders in the Brain of Streptozotocin-Induced Diabetic Male Rats.

Document Type : Original Article (s)

Authors

1 PhD Student, Department of Anatomy and Embryology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Associate Professor, Department of Anatomy and Embryology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Associate Professor, Department of Physiology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Associate Professor, Department of Biochemistry, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Background: Diabetes mellitus can lead to structural disorders in the brain. Aloe vera has antidiabetic, antioxidant, and neuroprotective effects. This study was designed to evaluate the effects of Aloe vera gel on the morphometric changes in the brain of streptozotocin-induced diabetic rats.Methods: 25 male Wistar rats were randomly divided into 5 groups including: control (normal saline), diabetic (normal saline), Aloe vera gel (400 mg/kg/day; gavage), diabetic + Aloe vera gel (400 mg/kg/day; gavage) and diabetic + Neutral Protamine Hagedorn (NPH) insulin (10 IU/kg/day; subcutaneous). Experimental diabetes was induced by streptozotocin injection (60 mg/kg; intraperitoneal). All groups treated for 8 weeks. At the end of treatment course, the rats' brains were removed intact immediately after sacrifice for morphometric measuring by caliper. Histological brain sections were also prepared to assess morphometry of cerebral cortex and hippocampus via ammonium iron (III) sulfate staining.Findings: Diabetes induction reduced weight and dimensions of brain, and diameters of the hippocampus, and white and gray matter of cerebral cortex compared to the control group after 8 weeks (P < 0.05); while treatment of diabetic rats with Aloe vera gel or insulin significantly decreased the structural alterations of the brain and improved the blood glucose level of diabetic rats (P < 0.05).Conclusion: The results showed that oral administration of Aloe vera gel in diabetic rats improves structural disorders of brain and ameliorates diabetes-induced hyperglycemia.

Keywords

References

  1. Forbes JM, Cooper ME. Mechanisms of diabetic complications. Physiol Rev 2013; 93(1): 137-88.
  2. Brands AM, Kessels RP, de Haan EH, Kappelle LJ, Biessels GJ. Cerebral dysfunction in type 1 diabetes: effects of insulin, vascular risk factors and blood-glucose levels. Eur J Pharmacol 2004; 490(1-3): 159-68.
  3. Jafari A, I, Barzegar GH, Pourheidar M. The protective effects of insulin and natural honey against hippocampal cell death in streptozotocin-induced diabetic rats. J Diabetes Res 2014; 2014: 491571.
  4. El-Akabawy G, El-Kholy W. Neuroprotective effect of ginger in the brain of streptozotocin-induced diabetic rats. Ann Anat 2014; 196(2-3): 119-28.
  5. Rajashree R, Kholkute SD, Goudar SS. Effects of duration of diabetes on behavioural and cognitive parameters in streptozotocin-induced juvenile diabetic rats. Malays J Med Sci 2011; 18(4): 26-31.
  6. Okouchi M, Ekshyyan O, Maracine M, Aw TY. Neuronal apoptosis in neurodegeneration. Antioxid Redox Signal 2007; 9(8): 1059-96.
  7. Huang TJ, Price SA, Chilton L, Calcutt NA, Tomlinson DR, Verkhratsky A, et al. Insulin prevents depolarization of the mitochondrial inner membrane in sensory neurons of type 1 diabetic rats in the presence of sustained hyperglycemia. Diabetes 2003; 52(8): 2129-36.
  8. Mao XY, Cao DF, Li X, Yin JY, Wang ZB, Zhang Y, et al. Huperzine A ameliorates cognitive deficits in streptozotocin-induced diabetic rats. Int J Mol Sci 2014; 15(5): 7667-83.
  9. Jangra A, Datusalia AK, Khandwe S, Sharma SS. Amelioration of diabetes-induced neurobehavioral and neurochemical changes by melatonin and nicotinamide: implication of oxidative stress-PARP pathway. Pharmacol Biochem Behav 2013; 114-115: 43-51.
  10. Musen G, Lyoo IK, Sparks CR, Weinger K, Hwang J, Ryan CM, et al. Effects of type 1 diabetes on gray matter density as measured by voxel-based morphometry. Diabetes 2006; 55(2): 326-33.
  11. Northam EA, Rankins D, Lin A, Wellard RM, Pell GS, Finch SJ, et al. Central nervous system function in youth with type 1 diabetes 12 years after disease onset. Diabetes Care 2009; 32(3): 445-50.
  12. Francis GJ, Martinez JA, Liu WQ, Xu K, Ayer A, Fine J, et al. Intranasal insulin prevents cognitive decline, cerebral atrophy and white matter changes in murine type I diabetic encephalopathy. Brain 2008; 131(Pt 12): 3311-34.
  13. Akaberi M, Sobhani Z, Javadi B, Sahebkar A, Emami SA. Therapeutic effects of Aloe spp. in traditional and modern medicine: A review. Biomed Pharmacother 2016; 84: 759-72.
  14. Benson KF, Newman RA, Jensen GS. Antioxidant, anti-inflammatory, anti-apoptotic, and skin regenerative properties of an Aloe vera-based extract of Nerium oleander leaves (nae-8((R))). Clin Cosmet Investig Dermatol 2015; 8: 239-48.
  15. Guven M, Golge UH, Aslan E, Sehitoglu MH, Aras AB, Akman T, et al. The effect of aloe vera on ischemia--Reperfusion injury of sciatic nerve in rats. Biomed Pharmacother 2016; 79: 201-7.
  16. Yuksel Y, Guven M, Kaymaz B, Sehitoglu MH, Aras AB, Akman T, et al. Effects of Aloe vera on spinal cord ischemia-reperfusion injury of rats. J Invest Surg 2016; 29(6): 389-98.
  17. Alinejad-Mofrad S, Foadoddini M, Saadatjoo SA, Shayesteh M. Improvement of glucose and lipid profile status with Aloe vera in pre-diabetic subjects: a randomized controlled-trial. J Diabetes Metab Disord 2015; 14: 22.
  18. Kim K, Kim H, Kwon J, Lee S, Kong H, Im SA, et al. Hypoglycemic and hypolipidemic effects of processed Aloe vera gel in a mouse model of non-insulin-dependent diabetes mellitus. Phytomedicine 2009; 16(9): 856-63.
  19. Fatemi Tabatabaei SR, Rashno M, Ghaderi S, Askaripour M. The aqueous extract of portulaca oleracea ameliorates neurobehavioral dysfunction and hyperglycemia related to streptozotocin-diabetes induced in ovariectomized rats. Iran J Pharm Res 2016; 15(2): 561-71.
  20. Gholami S, Saberi M. Histomorphometric alterations in aloe vera gel extract treatment in the diabetic rat’s retina. Comp Clin Path 2015; 24(5): 1021-9.
  21. Kuhad A, Chopra K. Tocotrienol attenuates oxidative-nitrosative stress and inflammatory cascade in experimental model of diabetic neuropathy. Neuropharmacology 2009; 57(4): 456-62.
  22. HEWITT W. A method for staining whole brains for gross and macroscopic study. J Anat 1959; 93(1): 134-6.
  23. Ahmadpour S, Haghir H, Sadeghi Y. Volumetric study of dentate gyrus and CA3 region in hippocampus of streptozotocin-induced diabetic rats: effect of insulin and ascorbic acid. Iran J Pathol 2008; 3(1): 1-4.
  24. Hernandez-Fonseca JP, Rincon J, Pedreanez A, Viera N, Arcaya JL, Carrizo E, et al. Structural and ultrastructural analysis of cerebral cortex, cerebellum, and hypothalamus from diabetic rats. Exp Diabetes Res 2009; 2009: 329632.
  25. Ramachandraiahgari R, Somesula S. Protective role of ethanolic extract of Aloe vera antioxidant properties on liver and kidney of streptozotocin-induced diabetic rats. Dig J Nanomater Biostruct 2012; 7(1): 175-84.
  26. Pothuraju R, Sharma RK, Onteru SK, Singh S, Hussain SA. Hypoglycemic and hypolipidemic effects of Aloe vera extract preparations: A review. Phytother Res 2016; 30(2): 200-7.
  27. Eltony SA. Histological study on the protective role of vitamin B complex on the cerebellum of diabetic rat. Tissue Cell 2016; 48(4): 283-96.
  28. Wang Y, Cao L, Du G. Protective effects of Aloe vera extract on mitochondria of neuronal cells and rat brain. Zhongguo Zhong Yao Za Zhi 2010; 35(3): 364-8. [In Chinese].
  29. Kocic R, Pavlovic D, Kocic G. Impact of intensive insulin treatment on the development and consequences of oxidative stress in insulin-dependent diabetes mellitus. Vojnosanit Pregl 2007; 64(9): 623-8.
  30. Wayhs CA, Mescka CP, Vanzin CS, Ribas GS, Guerreiro G, Nin MS, et al. Brain effect of insulin and clonazepam in diabetic rats under depressive-like behavior. Metab Brain Dis 2013; 28(4): 563-70.
Journal of Isfahan Medical School
Volume 35, Issue 422 - Serial Number 422
January and February 2017
Pages 235-242

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History

  • Receive Date: 01 January 2017
  • Revise Date:
  • Accept Date: 06 April 2022

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