تأثیر تمرینات تداومی و تناوبی شدید بر سطح عامل رشد عصبی مشتق از مغز (BDNF) و عامل نکروز تومور آلفا (TNF-α) در هیپوکامپ موش‌های صحرایی سالمند

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، واحد بجنورد، دانشگاه آزاد اسلامی، بجنورد، ایران

2 استادیار، گروه تربیت بدنی و علوم ورزشی، واحد بجنورد، دانشگاه آزاد اسلامی، بجنورد، ایران

3 استادیار، مرکز تحقیقات فراورده‌های طبیعی و گیاهان دارویی، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران

چکیده

مقدمه: هدف از انجام مطالعه‌ی حاضر، بررسی اثر تمرینات تداومی و تناوبی شدید بر سطح Brain-derived neurotrophic factor (BDNF) و Tumor necrosis factor (TNF-α) در هیپوکامپ موش‌های صحرایی سالمند بود.روش‌ها: 30 سر موش سالمند نر Wisatr (سن 18 ماه) به‌طور تصادفی و بر اساس وزن به سه گروه تمرین تناوبی، تمرین تداومی و شاهد تقسیم شدند. تمرین تداومی به مدت 8 هفته با شدت 70-65 درصد VO2maxو شیوه‌نامه‌ی تمرین تناوبی نیز به مدت 8 هفته با اجرای 8-5 مرحله فعالیت 2 دقیقه‌ای با شدت معادل 100-80 درصد VO2max و با دوره‌های استراحتی فعال 2 دقیقه‌ای، اجرا شد. نمونه‌های بافت هیپوکامپ 48 ساعت پس از آخرین جلسه‌ی تمرین استخراج شد. از آزمون One-way ANOVA برای تحلیل داده‌ها استفاده شد.یافته‌ها: سطح پروتئین BDNF هیپوکامپ در گروه تمرین تداومی (002/0 > P) و تناوبی (003/0 > P) به طور معنی‌داری نسبت به گروه شاهد بالاتر بود، اما بین سطح پروتئین BDNF هیپوکامپ در گروه تمرین تداومی و تمرین تناوبی تفاوت معنی‌داری مشاهده نشد (361/0 = P). سطح پروتئین TNF-α هیپوکامپ در گروه تمرین تداومی و تناوبی به طور معنی‌داری نسبت به گروه شاهد بالاتر بود (001/0 = P برای هر دو). بین سطح پروتئین TNF-αهیپوکامپ در گروه تمرین تداومی و تمرین تناوبی تفاوت معنی‌داری مشاهده نشد (639/0 = P).نتیجه‌گیری: تمرین تداومی و تناوبی روی نوار گردان از طریق افزایش سطوح TNF-α، محتوای BDNF هیپوکامپ موش‌های صحرایی نر سالمند را افزایش می‌دهد و بنابراین، تمرینات ورزشی تداومی و تناوبی شدید، می‌تواند از تحلیل سیستم عصبی ناشی از سالمندی، از طریق بهبود سطح BDNF جلوگیری کند.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Intense Continuous and Interval Training on the Levels of Brain-Derived Neurotrophic Factor (BDNF) and Tumor Necrosis Factor Alpha (TNF- α) in Hippocampus of Old Rats

نویسندگان [English]

  • Maysa Moradi 1
  • Ali Yaghoubi 2
  • Mohammad-Reza Jalilvand 3
1 Department of Physical Education and Sport Science, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran
2 Assistant Professor, Department of Physical Education and Sport Science, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran
3 Assistant Professor, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
چکیده [English]

Background: The present study aimed to evaluate the effect of intense continuous and interval training on the levels of Brain-Derived Neurotrophic Factor (BDNF) and Tumor Necrosis Factor Alpha (TNF-α) in hippocampus of old rats.Methods: 30 old male Wistar rats (18 weeks old) were divided into 3 groups of interval training, continuous training, and control. Continuous training was performed for 8 weeks with 65% to 70% VO2max and interval training was performed 8 weeks with 5-8 repetitions of 2 minutes of working with 80%-100% VO2max and 2 minutes active rest with 50% of VO2max. Hippocampal samples were extracted 48 hours after the last training session to measure protein levels of BDNF and TNF-α. One-way ANOVA and Tukey post hoc tests were used for data analysis.Findings: Hippocampus BDNF levels in continuous (P = 0.002) and interval training (P = 0.003) groups were significantly higher than the control group. But no significant differences were found in BDNF levels between continuous and interval training groups (P = 0.361). TNF-α levels in continuous and interval training groups were significantly higher than the control group (P = 0.001 for both). But no significant differences were found in TNF-α levels between continuous and interval training groups (P = 0.639).Conclusion: Intense continuous and interval training increase BDNF levels in the hippocampus of the elderly rats through increasing TNF-α levels; thus, intense continuous and interval training can prevent aging of the nervous system by improving BDNF levels.

کلیدواژه‌ها [English]

  • High-Intensity Interval Training
  • Brain-derived neurotrophic factor
  • Tumor Necrosis Factor-alpha
  • Rats

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  • تاریخ دریافت: 04 اردیبهشت 1400
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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