تأثیر هشت هفته تمرین مقاومتی و استقامتی بر شاخص‌های آپوپتوزی مردان جوان

مرادیان, فهیمه; نظرعلی, پروانه; علی‌زاده, رستم

doi:10.22122/jims.v36i489.10268

تأثیر هشت هفته تمرین مقاومتی و استقامتی بر شاخص‌های آپوپتوزی مردان جوان

نوع مقاله : مقاله های پژوهشی

نویسندگان

¹ گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه الزاهرا (س)، تهران، ایران

² دانشیار، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه الزاهرا (س)، تهران، ایران

³ استادیار، گروه علوم ورزشی، دانشکده‌ی ادبیات و علوم انسانی، دانشگاه ایلام، ایلام، ایران

10.22122/jims.v36i489.10268

چکیده

مقدمه: آپوپتوزیس (Apoptosis)، فرایندگی است که به تازگی مورد توجه محققان علوم ورزشی نیز قرار گرفته است؛ چرا که مشاهده شده است که در زمان ورزش، علاوه بر نکروز، آپوپتوز نیز رخ می‌دهد. از این رو، تحقیق حاضر طراحی شد تا سازگاری احتمالی فرایند آپوپتوز را بعد از هشت هفته تمرین مقاومتی و استقامتی بررسی و مقایسه کند.روش‌ها: تعداد 21 دانشجوی خوابگاهی پسر به طور تصادفی در سه گروه تمرین استقامتی، مقاومتی و شاهد تقسیم شدند. تمرینات به مدت هشت هفته و با توالی سه جلسه در هفته اجرا شد. قبل و بعد از دوره‌ی تمرینی، نمونه‌ی خونی گرفته شد و مقادیر BCI2 و BAX با روش Enzyme-linked immunosorbent assay (ELISA) اندازه‌گیری شد. داده‌ها با استفاده از آزمون آماری One-way ANOVA در سطح معنی‌داری 05/0 > P تجزیه و تحلیل شدند.یافته‌ها: تفاوت معنی‌دار در تغییرات عامل BCI2 بین سه گروه (002/0 = P) وجود داشت؛ به نحوی که بین گروه استقامتی با گروه شاهد (002/0 = P) و گروه استقامتی با گروه مقاومتی (014/0 = P) تفاوت معنی‌داری وجود داشت، اما بین گروه مقاومتی با گروه شاهد (770/0 = P) تفاوت معنی‌داری وجود نداشت، اما برای BAX (216/0 = P) و نسبت BCL-2/BAX (092/0 = P) تفاوت معنی‌داری مشاهده نشد.نتیجه‌گیری: تحقیق حاضر نشان داد که هشت هفته تمرین ورزشی صرف ‌نظر از نوع آن، باعث ایجاد تأثیرات مثبت در عوامل آپوپتوزی می‌گردد. نوع تمرین نیز در میزان و نوع تغییرات مؤثر می‌باشد. از این رو، به نظر می‌رسد داشتن تمرین ورزشی منظم با شدت متوسط مهم‌تر از نوع آن در ارتباط با سیستم آپوپتوزی در بدن باشد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Eight Weeks of Endurance and Resistance Training on Apoptotic Indexes in Young Men

نویسندگان [English]

Fahimeh Moradian ¹
Parvaneh Nazarali ²
Rostam Alizadeh ³

¹ Department of Exercise Physiology, School of Sport Sciences, Alzahra University, Tehran, Iran

² Associate Professor, Department of Exercise Physiology, School of Sport Sciences, Alzahra University, Tehran, Iran

³ Assistant Professor, Department of Sports Sciences, School of Literature and Humanities, Ilam University, Ilam, Iran

چکیده [English]

Background: Apoptosis has recently sparked interest among sport science researchers; since they have found out, both necrosis and apoptosis tend to occur during training. The present research was, therefore, designed to investigate and compare the possible adaptation of the apoptotic process following eight weeks of endurance and resistance training.Methods: Twenty-one dormitory boy students were randomly assigned to three groups of endurance, resistance, or control. The training was performed for eight weeks, three sessions per week. Blood samples were taken before and after the training, and BC12 and BAX indexes were measured using the enzyme-linked immunosorbent assay (ELISA) method. The data were analyzed using a one-way ANOVA, with the significance level of P < 0.050.Findings: There was a significant difference in BC12 changes between the three groups (P = 0.002), as well as between the endurance with control (P = 0.002) and resistance with endurance (P = 0.014) groups. But there was no significant difference between the resistance and control groups (P = 0.770). No significant differences were observed in BAX (P = 0.216) and the BCL2/BAX ratio (P = 0.092) between the groups.Conclusion: The present study showed that eight weeks of training, irrespective of training type, could result in positive effects on the apoptotic factors. Training type is also considered as effective in the scale and type of changes. Therefore, it seems that, with respect to the apoptotic process in the body, regular exercise with normal intensity could take on a greater importance than the type of training.

کلیدواژه‌ها [English]

Apoptosis
Men
Exercise

مراجع

Sari-Sarraf V, Amirsasan R, Sheikholeslami-Vatani D, Faraji H. Effect of creatine supplementation on the factors involved in apoptosis-related process (Bax, Bcl-2) and their ratio (Bcl-2/Bax) during acute resistance exercise in middle-aged men. Sci J Kurdistan Univ Med Sci 2016; 21(4): 17-28. [In Persian].
Phaneuf S, Leeuwenburgh C. Apoptosis and exercise. Med Sci Sports Exerc 2001; 33(3): 393-6.
Colliander EB, Tesch PA. Effects of eccentric and concentric muscle actions in resistance training. Acta Physiol Scand 1990; 140(1): 31-9.
Johnston AP, De Lisio M, Parise G. Resistance training, sarcopenia, and the mitochondrial theory of aging. Appl Physiol Nutr Metab 2008; 33(1): 191-9.
Hudson MB, Hosick PA, McCaulley GO, Schrieber L, Wrieden J, McAnulty SR, et al. The effect of resistance exercise on humoral markers of oxidative stress. Med Sci Sports Exerc 2008; 40(3): 542-8.
Ahmadiasl N, Soufi FG, Alipour M, Bonyadi M, Sheikhzadeh F, Vatankhah A, et al. Effects of age increment and 36-week exercise training on antioxidant enzymes and apoptosis in rat heart tissue. J Sports Sci Med 2007; 6(2): 243-9.
Cooke WH, Carter JR. Strength training does not affect vagal-cardiac control or cardiovagal baroreflex sensitivity in young healthy subjects. Eur J Appl Physiol 2005; 93(5-6): 719-25.
Ghanbari Niaki A, Ardeshiri S, Aliakbari Baydokhty M, Saeidi A. Effects of circuit resistance training with crocus sativus supplementation on insulin and estradiol hormones response. The Horizon of Medical Sciences 2016; 22(2): 125-30.
Agha Alinejad H, Mehrabani J, Ansari Dogahe R, Piri M. The influence of resistance, endurance, and combined resistance-endurance exercise training on interleukin-18 and c-reactive protein level in inactive female adolescents. Tabari J Prev Med 2016; 2(1):38-47.
Oltvai ZN, Milliman CL, Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 1993; 74(4): 609-19.
Marsh SA, Laursen PB, Pat BK, Gobe GC, Coombes JS. Bcl-2 in endothelial cells is increased by vitamin E and alpha-lipoic acid supplementation but not exercise training. J Mol Cell Cardiol 2005; 38(3): 445-51.
Youle RJ, Strasser A. The BCL-2 protein family: Opposing activities that mediate cell death. Nat Rev Mol Cell Biol 2008; 9(1): 47-59.
Siu PM, Bryner RW, Martyn JK, Alway SE. Apoptotic adaptations from exercise training in skeletal and cardiac muscles. FASEB J 2004; 18(10): 1150-2.
Delchev SD, Georgieva KN, Koeva YA, Atanassova PK. Bcl-2/Bax ratio, mitochondrial membranes and aerobic enzyme activity in cardiomyocytes of rats after submaximal training. Folia Med (Plovdiv) 2006; 48(2): 50-6.
Lu K, Wang L, Wang C, Yang Y, Hu D, Ding R. Effects of high-intensity interval versus continuous moderate-intensity aerobic exercise on apoptosis, oxidative stress and metabolism of the infarcted myocardium in a rat model. Mol Med Rep 2015; 12(2): 2374-82.
Childs AC, Phaneuf SL, Dirks AJ, Phillips T, Leeuwenburgh C. Doxorubicin treatment in vivo causes cytochrome C release and cardiomyocyte apoptosis, as well as increased mitochondrial efficiency, superoxide dismutase activity, and Bcl-2:Bax ratio. Cancer Res 2002; 62(16): 4592-8.
Mooren FC, Bloming D, Lechtermann A, Lerch MM, Volker K. Lymphocyte apoptosis after exhaustive and moderate exercise. J Appl Physiol (1985) 2002; 93(1): 147-53.
Brocheriou V, Hagege AA, Oubenaissa A, Lambert M, Mallet VO, Duriez M, et al. Cardiac functional improvement by a human Bcl-2 transgene in a mouse model of ischemia/reperfusion injury. J Gene Med 2000; 2(5): 326-33.
Marin-Garcia J, Goldenthal MJ. Mitochondrial centrality in heart failure. Heart Fail Rev 2008; 13(2): 137-50.
Kavazis AN, McClung JM, Hood DA, Powers SK. Exercise induces a cardiac mitochondrial phenotype that resists apoptotic stimuli. Am J Physiol Heart Circ Physiol 2008; 294(2): H928-H935.
French JP, Hamilton KL, Quindry JC, Lee Y, Upchurch PA, Powers SK. Exercise-induced protection against myocardial apoptosis and necrosis: MnSOD, calcium-handling proteins, and calpain. FASEB J 2008; 22(8): 2862-71.
Kwak HB, Song W, Lawler JM. Exercise training attenuates age-induced elevation in Bax/Bcl-2 ratio, apoptosis, and remodeling in the rat heart. FASEB J 2006; 20(6): 791-3.
Sun Y, Cui D, Zhang Z, Shi J, Jin H, Ge Z, et al. Attenuated oxidative stress following acute exhaustive swimming exercise was accompanied with modified gene expression profiles of apoptosis in the skeletal muscle of mice. Oxid Med Cell Longev 2016; 2016: 8381242.
Mejias-Pena Y, Estebanez B, Rodriguez-Miguelez P, Fernandez-Gonzalo R, Almar M, de Paz JA, et al. Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects. Aging (Albany NY) 2017; 9(2): 408-18.
Santana ET, Serra AJ, Silva Junior JA, Bocalini DS, Barauna VG, Krieger JE, et al. Aerobic exercise training induces an anti-apoptotic milieu in myocardial tissue. Motriz: Rev Educ Fis 2014; 20(2): 233-8.
Martinou JC. Apoptosis. Key to the mitochondrial gate. Nature 1999; 399(6735): 411-2.
Ziaaldini MM, Koltai E, Csende Z, Goto S, Boldogh I, Taylor AW, et al. Exercise training increases anabolic and attenuates catabolic and apoptotic processes in aged skeletal muscle of male rats. Exp Gerontol 2015; 67: 9-14.
Kruger K, Mooren FC. Exercise-induced leukocyte apoptosis. Exerc Immunol Rev 2014; 20: 117-34.
Cooper DM. The balance between life and death: Defining a role for apoptosis in aging. J Clin Exp Pathol 2012; S4: 001.
Skommer J, Wlodkowic D, Deptala A. Larger than life: Mitochondria and the Bcl-2 family. Leuk Res 2007; 31(3): 277-86.
Quadrilatero J, Alway SE, Dupont-Versteegden EE. Skeletal muscle apoptotic response to physical activity: Potential mechanisms for protection. Appl Physiol Nutr Metab 2011; 36(5): 608-17.