ساخت و ارزیابی نانوداربست کتیرا/ پلی کاپرولاکتان غنی شده با سیلیمارین، حاوی سلول‌های دندانی جهت کاربرد در مهندسی بافت

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه زیست‌شناسی، دانشکده‌ی علوم، دانشگاه محقق اردبیلی، اردبیل، ایران

2 دانشیار، گروه زیست‌شناسی، دانشکده‌ی علوم، دانشگاه محقق اردبیلی، اردبیل، ایران

3 استاد، گروه زیست‌شناسی، دانشکده‌ی علوم، دانشگاه محقق اردبیلی، اردبیل، ایران

4 دانشیار، گروه بیوفیزیک، دانشکده‌ی فناوری‫های نوین، دانشگاه محقق اردبیلی، اردبیل، ایران‬‬‬‬

چکیده

مقاله پژوهشی




مقدمه: مهندسی بافت، مجموعه‌ای از روش‌هایی است که می‌تواند بافت‌های آسیب دیده را با بافت طبیعی یا مصنوعی جایگزین یا ترمیم کند. کتیرا، یک پلیمر طبیعی است که خواص بیولوژیکی عالی مانند تجزیه‌ی زیستی و توانایی زیست‌سازگاری دارد. سیلیمارین از نظر بیوشیمیایی دارای خواص پاک‌کننده و آنتی‌اکسیدانی است و همچنین اثرات ضد التهابی دارد. هدف از این مطالعه، تولید نانوداربست پلی کاپرولاکتان (Polycaprolactone) PCL /کتیرا/ سیلیمارین و بررسی زیست سازگاری سلول‌های دندانی بر روی آن می‌باشد.
روش‌ها: به منظور تهیه‌ی نانوداربست پلی کاپرولاکتان/کتیرا و بارگذاری سیلیمارین بر روی آن، محلول پلی‌کاپرولاکتان 7 درصد (حل شده در استیک اسید)، محلول کتیرا
7/0 درصد وزنی و محلول سیلیمارین با غلظت 0/9 درصد وزنی مخلوط شد، سپس توسط دستگاه الکتروریسی داربست تهیه شد. مورفولوژی داربست توسط میکروسکوب الکترونی روبشی (Scanning electron microscope) SEM و ساختار شیمیایی داربست توسط طیف‌سنجی FTIR مورد ارزیابی قرار گرفت.
یافته‌ها: بررسی مورفولوژی داربست و ساختار شیمیایی آن نشان‌دهنده‌ی تخلخل مناسب داربست پلی کاپرولاکتان و بارگذاری موفق سیلیمارین بر روی داربست بود. زیست سازگاری داربست 24، 48 و 72 ساعت بعد از کشت سلول‌های دنتال مورد بررسی قرار گرفت که نتایج نشان‌دهنده افزایش زنده‌مانی سلول‌ها و اتصال مناسب سلول‌ها بر روی داربست بود.
نتیجه‌گیری: نتایج حاصل از این پژوهش نشان داد که بارگذاری سیلیمارین بر روی داربست پلی کاپرولاکتان/کتیرا باعث افزایش توان تکثیر و زنده‌مانی سلول‌های دندانی می‌شود. از این‌رو این داربست می‌تواند کاندید مناسبی برای مهندسی بافت باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Fabrication and Evaluation of Silymarin-Enriched Tragacanth / Polycaprolactone Nanoscaffold Containing Dental Cells for Use in Tissue Engineering

نویسندگان [English]

  • Reza Najafi 1
  • Asadollah Asadi 2
  • Saber Zahri 3
  • Arash Abdolmaleki 4
1 PhD Candidate, Department of Biology, School of Science, University of Mohaghegh Ardabili, Ardabil, Iran
2 Associate professor, Department of Biology, School of Science, University of Mohaghegh Ardabili, Ardabil, Iran
3 Professor, Department of Biology, School of Science, University of Mohaghegh Ardabili, Ardabil, Iran
4 Associate professor, Department of Biophysics, School of Advanced Technologies, University of Mohaghegh Ardabili, Ardabil, Iran
چکیده [English]

Background: Tissue engineering is a set of methods that can replace or repair damaged tissues with natural or artificial tissue. Tragacanth is a natural polymer that has excellent biological properties such as biodegradability and biocompatibility. Silymarin biochemically has cleansing and antioxidant properties and also has anti-inflammatory effects. The aim of this study is the production of polycaprolactone (PCL) / tragacanth / silymarin nanoscaffold and to investigate the biocompatibility of dental cells on it.
Methods: To create a polycaprolactone /tragacanth nanoscaffold and add silymarin to it, acetic acid was used to dissolve 7 percent of the polycaprolactone, 0.7 weight percent of the tragacanth solution, and 0.9 percent of the silymarin solution. The scaffold was then created using an electrospinning machine. Scanning electron microscope (SEM) analysis and FTIR analysis were used to analyze the scaffold's chemical structure and shape, respectively.
Findings: The scaffold's correct porosity and the successful loading of silymarin on it were revealed by analyzing the scaffold's morphology and chemical composition. The biocompatibility of the scaffold was investigated 24, 48 and 72 hours after the cultivation of dental cells, and the results showed an increase in cell viability and proper attachment of cells on the scaffold.
Conclusion: The findings of this study demonstrated that silymarin loading on the polycaprolactone/catira scaffold improves dental cell proliferation and survival. This scaffold may therefore be a good choice for tissue engineering.

کلیدواژه‌ها [English]

  • Polycaprolactone
  • Silymarin
  • Tissue engineering
  • Tragacant
  • Electrospinning
  • Dental cells

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مجله دانشکده پزشکی اصفهان
دوره 41، شماره 714
هفته 2 خرداد
خرداد و تیر 1402
صفحه 226-233

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