Isolation of Sarcocystis Hirsuta from Traditional Hamburger of Iran

Document Type : Original Article (s)

Authors

1 Assistant Professor, Department of Food Hygiene and Safety, School of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

2 Assistant Professor, Department of Biostatistics and Epidemiology, School of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

3 Department of Food Hygiene and Safety, School of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

4 Assistant Professor, Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

5 Professor, Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

6 Assistant Professor, Department of Food Science and Technology, Islamic Azad University, Science and Research Branch, Yazd, Iran

7 Department of Laboratory Sciences, School of Para-Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Abstract

Background: Sarcocystosis is one of the common zoonotic diseases caused by the parasites of genus Sarcocystis. Sarcocystis is a two-host intracellular protozoan of the Phylum Apicomplexa. Three known species of Sarcosystis can infect cattle as intermediate host: Sarcocystis cruzi, Sarcosystis hirsute, and Sarcosystis hominis that dogs, cats, and humans are their final hosts, respectively.Methods: In this study, a sample of traditional hamburger was purchased from a street food seller in Yazd, Iran. DNA was extracted by salting-out method. To identify Sarcocystis and by using specific primers, fragment of genomic DNA (18s rRNA) was amplified and results of electrophoresis showed the polymerase chain reaction (PCR) product with about 953 bp in length indicating presence of the Sarcocystis genus. To identify the species of the parasite, two enzymes, RsaI and BfaI, were used for cutting the amplified fragment. Results of digestion were analyzed using agarose gel electrophoresis.Findings: After digestion with BfaI, the results of gel agarose electrophoresis showed 557 and 397 bp in length bands. Two bands of 577 and 376 bp in length were found after using RsaI that indicated presence of Sarcocystis hirsuta.Conclusion: In this report, Sarcocystis hirsuta was identified in Iranian traditional hamburger that could be related to close association of dogs and cats in farms that is the main reason that food and water are contaminated with feces of cats. To the best of our knowledge, this study is the first report of Sarcocystis hirsuta infection in Iranian traditional hamburger.

Keywords

References

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Journal of Isfahan Medical School
Volume 32, Issue 273 - Serial Number 273
January and February 2014
Pages 79-85

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History

  • Receive Date: 04 December 2013
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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