Production of YY Peptides in the Escherichia Coli Expression System Using Self-Induction Promoter

Document Type : Original Article (s)

Authors

1 PhD Student, Department of Microbiology, School of Basic Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran

2 Assistant Professor, Department of System Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

3 Assistant Professor, Department of Microbiology, School of Basic Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran

Abstract

Background: Application of toxic inducers, i.e. isopropyl β-D-1-thiogalactopyranoside (IPTG), for production of biopharmaceuticals has been prohibited by regulatory agencies. Here, we sought to evaluate the feasibility of a self-induced expression system for production of YY(3-36) peptide as an active pharmaceutical ingredient (API) in the Escherichia coli expression system.Methods: The sequence of self-induced promoter was obtained from Data banks, and synthetized in pUC18 vector. The nucleotide sequence of YY(3-36) was inserted downstream of the promoter, after the 22 residue of asparaginase II signal sequence. The expression of the peptide was compared with pET21 harboring the same construct under isopropyl β-D-1-thiogalactopyranoside induction in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).Findings: The expression of target peptide was comparable with isopropyl β-D-1-thiogalactopyranoside (1 mM) expressed peptide.Conclusion: Our results demonstrate the ease and economical application of used self-induced promoter as an alternative system in production of high value added biopharmaceuticals.

Keywords


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