تأثیر تمرینات زودهنگام استقامتی بر بهبود ضایعات مغزی در ناحیه‌ی CA1 هیپوکامپ و بیان پروتئین A2A به دنبال سکته‌ی ایسکمیک مغزی در موش صحرایی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، ‌دانشکده‌ی علوم انسانی، واحد ‌بجنورد، ‌دانشگاه ‌آزاد ‌اسلامی، ‌بجنورد، ‌ایران

2 دانشیار، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه مازندران، ساری، ایران

3 دانشیار، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه فردوسی مشهد، مشهد، ایران

4 استادیار، گروه‌ فیزیولوژی ورزش، دانشکده‌ی تربیت بدنی و علوم ورزشی، ‌واحد ‌بجنورد، ‌دانشگاه‌ آزاد ‌اسلامی،‌ بجنورد،‌ ایران

5 دکتری تغذیه، مرکز تحقیقات سلامت غذایی (نمک)، دانشگاه علوم پزشکی سمنان، سمنان، ایران

چکیده

مقدمه: ایسکمی/رپرفیوژن مغزی، باعث ایجاد آسیب‌های ساختاری و عملکردی در هیپوکامپ می‌شود. فعالیت فیزیکی، یک روش ضروری و کارآمد در توان‌بخشی زودهنگام پس از سکته‌ی مغزی است. هدف از انجام این مطالعه، بررسی تأثیر ۸ هفته تمرین استقامتی زودهنگام بر روی تردمیل، بر بهبود نورون‌های ناحیه‌ی CA1 هیپوکامپ و بیان پروتئین A2A به دنبال ایسکمی/رپرفیوژن مغزی در موش صحرایی نر بود.روش‌ها: ۲۱ سر موش صحرایی نر نژاد Wistar به طور تصادفی به سه گروه (Sham، ایسکمی و کنترل و نیز ایسکمی و تمرین) تقسیم شدند. موش‌های گروه ایسکمی و تمرین، ۲۴ ساعت پس از القای ایسکمی به مدت ۸ هفته، ۵ روز در هفته روی نوار گردان دویدند. ایسکمی با انسداد هر دو شریان کاروتید مشترک به مدت ۴۵ دقیقه ایجاد شد. از آزمون Ladder برای بررسی وضعیت عملکردی و از رنگ‌آمیزی هماتوکسیلین- ائوزین (H&E) جهت بررسی میزان مرگ نورونی استفاده گردید. همچنین، جهت بررسی بیان پروتئینی، از روش Immunohistochemistry استفاده شد.یافته‌ها: ایسکمی مغزی، با افزایش مرگ سلول‌های عصبی ناحیه‌ی CA1 هیپوکامپ و کاهش عملکرد شناختی و یادگیری حرکتی در حیوانات همراه بود. تمرین زودهنگام به طور معنی‌داری باعث کاهش مرگ سلولی و کاهش اختلال شناختی و یادگیری حرکتی شد. همچنین، بیان پروتئین A2A در گروه ایسکمی وتمرین نسبت به گروه ایسکمی + کنترل، تفاوت معنی‌داری نداشت.نتیجه‌گیری: تمرین زودهنگام روی نوار گردان، به عنوان یک محرک توان‌بخشی، موجب اثرات محافظتی در برابر مرگ سلولی و بهبود عملکرد حرکتی خواهد شد.

کلیدواژه‌ها

عنوان مقاله [English]

Impact of Early Endurance Training on Improvement of Brain Damage in CA1 Region of Hippocampus and Expression of A2A Protein Following Ischemic Stroke in Rats

نویسندگان [English]

  • Mehdi Seydyousefi 1
  • Ziya Fallahmohammadi 2
  • Mahtab Moazzami 3
  • Ali Yaghoubi 4
  • Zeinab Faghfoori 5
1 Department of Physical Education and Sport Sciences, School of Humanities, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran
2 Associate Professor, Department of Exercise Physiology, School of Sport Sciences, University of Mazandaran, Sari, Iran
3 Associate Professor, Department of Exercise Physiology, School of Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
4 Assistant Professor, Department of Physical Education and Sport Sciences, School of Humanities, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran
5 PhD of Nutrition, Food Safety Research Center (Salt), Semnan University of Medical Sciences, Semnan, Iran
چکیده [English]

Background: Brain ischemia/reperfusion causes structural and functional damage in the hippocampus. Physical activity is an essential method for early rehabilitation after a stroke. The aim of this study was to determine the effect of 8 weeks of early endurance training on treadmill on improvement of the neurons of CA1 region of hippocampus and expression of A2A protein following cerebral ischemia/reperfusion in male rats.Methods: 21 male Wistar rats were randomly divided into three groups of sham, ischemia + control, and ischemia + exercise. 24 hours after induction of ischemia, the rats in exercise group ran 5 days a week, for 8 weeks. Ischemia was induced by blocking both common carotid arteries for 45 minutes. Ladder test, hematoxylin and eosinophil (H&E) staining, and immunohistochemical method were used to evaluate functional status, neuronal death, and protein expression, respectively.Findings: The brain ischemia was associated with an increase in neuronal death in the CA1 region of hippocampus, and reduced cognitive function and motor learning. Early training significantly decreased cell death, as well as impaired cognitive function and motor learning. Moreover, in ischemia + exercise group, expression of A2A did not change significantly compared to the ischemia + control group.Conclusion: This study showed that early training on treadmill, as a rehabilitation stimulant, would have protective effects against cell death, and can improve motor function.

کلیدواژه‌ها [English]

  • Endurance training
  • Ischemia
  • Reperfusion

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مجله دانشکده پزشکی اصفهان
دوره 37، شماره 526 - شماره پیاپی 526
خرداد و تیر 1398
صفحه 485-492

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سابقه مقاله

  • تاریخ دریافت: 01 تیر 1398
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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