نقش نواحی قشر جلوی پیشانی و تگمنتوم شکمی بر علایم سندرم ترک در موش‌های صحرایی نر معتاد به مرفین

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری تخصصی فیزیولوژی جانوری، گروه زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 استاد، گروه فیزیولوژی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

3 استاد، گروه زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی،تهران، ایران

4 دانشیار، گروه فیزیولوژی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

مقدمه: از عوامل مشکل‌ساز در درمان اعتیاد، پدیده‌ی عود علایم ترک در فرد معتاد می‌باشد. نواحی شکل دهنده‌ی مسیر پاداشی مغز مانند قشر جلوی پیشانی و ناحیه‌ی تگمنتوم شکمی در ایجاد وابستگی و علایم ترک دخیل می‌باشند. این مطالعه، جهت بررسی ارتباط این نواحی در بروز و تخفیف علایم ترک، تحریک و تخریب الکتریکی ناحیه‌ی قشر جلوی پیشانی (Medial prefrontal cortex یا mPFC) و همچنین، بلوکه کردن گیرنده‌های گلوتاماتی در ناحیه‌ی تگمنتوم شکمی (Ventral tegmental area یا VTA) انجام گردید.روش‌ها: در یک مطالعه‌ی تجربی، از 42 سر موش صحرایی نر با میانگین وزنی 300-250 گرم در شش گروه هفت‌تایی شامل گروه‌های شاهد، مرفین، دریافت ‌کننده‌ی تحریک و تخریب الکتریکی در ناحیه‌ی mPFC و گروه‌های دریافت کننده‌ی میکرواینجکشن آنتاگونیست‌های گیرنده‌های گلوتاماتدر ناحیه‌ی VTA استفاده گردید. تمامی گروه‌ها، (غیر از گروه شاهد)، مرفین درون‌ صفاقی را به مدت نه روز طبق شیوه‌نامه‌ دریافت ‌کردند. سپس، در روز دهم نشانگان ترک در حیوانات بررسی ‌گردید.یافته‌ها: تحریک الکتریکی ناحیه‌ی mPFC، سبب افزایش معنی‌دار (050/0 > P) رفتارهای چرخش و خاراندن و کشش بدن (010/0 > P) نسبت به گروه مرفین گردید. در حالی که تخریب این ناحیه و همچنین، میکرواینجکشن آنتاگونیست‌های گلوتاماتی در VTA، منجر به کاهش معنی‌دار کلیه‌ی علایم سندرم ترک مرفین در موش‌های‌صحرایی شد.نتیجه‌گیری: تحریک الکتریکی ناحیه‌ی mPFC علایم سندرم ترک در موش‌های ‌صحرایی معتاد را افزایش داد که به احتمال قوی، از طریق افزایش انتقالات گلوتاماترژیکی به ناحیه‌ی VTA و تقویت مسیر پاداشی میسر گردیده بود. در صورتی که احتمال می‌رود تخریب این ناحیه و بلوک گیرنده‌های گلوتاماتی، با تضعیف ارسالات گلوتاماترژیکی، منجر به کاهش وابستگی به مرفین گردیده است.

کلیدواژه‌ها

عنوان مقاله [English]

The Role of Medial Prefrontal Cortex and Ventral Tegmental Areas in Withdrawal Syndrome in Morphine Addicted Male Rats

نویسندگان [English]

  • Asal Keramatian 1
  • Hojjatallah Alaei 2
  • Akram Eidi 3
  • Maryam Radahmadi 4
1 PhD Student, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Professor, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 Associate Professor, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده [English]

Background: One of the problems of addiction treatment is the relapsing of withdrawal syndrome signs in the addicted person. Rewarding brain regions such as medial prefrontal cortex (mPFC) and ventral tegmental area (VTA) are involved in addiction and onset of withdrawal syndrome. This study was performed to investigate the relationship between these areas in demonstration or relief of withdrawal signs, electrical stimulation and lesion of mPFC, as well as blocking the relative receptors in the VTA.Methods: In an experimental study, 42 male rats weighing 250-300 g were divided in 6 equal groups of control, morphine, and stimulation and lesion electrical receiver groups in mPFC region, and groups receiving the glutamate receptors antagonists by microinjection into the VTA. All of the groups, except the control group, received intraperitoneal morphine during 9 days as the protocol. Finally, on the 10th day, the symptoms of addiction in rats were evaluated.Findings: The electrical stimulation of mPFC significantly increased cycling and scratching (P < 0.01) and bodylifting (P < 0.05) in comparison to the morphine group. Whereas the lesion of this area, and also microinjection of glutamate antagonists into the VTA caused significant decrease of all the withdrawal symptoms in rats.Conclusion: Electrical stimulation of mPFC area increased the withdrawal symptoms in addicted rats, which was most likely due to increase of glutamatergic transmissions into the VTA and reinforcement of reward system; while destruction of this area and blocking of glutamate receptors, probably via decreasing of glutamatergic transmissions, caused decrease of morphine addiction.

کلیدواژه‌ها [English]

  • Prefrontal cortex
  • Ventral tegmental area
  • Withdrawal syndrome

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  • تاریخ دریافت: 19 تیر 1399
  • تاریخ بازنگری: 12 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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