Association between Polymorphic promoter region in the IGF-I and risk of breast cancer in Isfahan area

Document Type : Original Article (s)

Authors

1 Student, Department of Biology, School of Sciences, The Uneversity of Isfahan, Isfahan, Iran

2 Associate Professor, Department of Biology, School of Sciences, The Uneversity of Isfahan, Isfahan, Iran

3 Assistant Professor, Department of Radiotherapy, School of Medicine, School Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Insulin like growth factor I (IGF-I) is a member of large single-chain polypeptides, similar to proinsulin protein. This gene increases cell growth. The purpose of this study was to investigate polymorphism of Adenosine-Cytosine (CA) in IGF-I gene promoter among breast cancer patients and healthy individuals and its relation to risk of breast cancer.Methods: This study was a case-control study on 100 patients and 100 controls women. DNA was extracted from the blood of study subjects, the sequence were amplified by the technique of Polymerase Chain Reaction (PCR). Thereafter the number and sequence of CA was measured by gel electrophoresis on Polyacrilamid.Finding: The results of this study shown that IGF-I gene allele distribution in Isfahan population varies between 11 and 23 repeats and (CA)19 was the most common allele between patients and controls. Women with sequences larger than 19 were in a greater risk of breast cancer.Conclusion: These findings indicate a direct relationship between the numbers of repetitive sequences of IGF-I gene promoter and increased risk of breast cancer. In other words, increasing the number of CA repeat (greater than 19) located in the promoter gene, increases the risk of breast cancer in women.

Keywords

References

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Journal of Isfahan Medical School
Volume 28, Issue 119 - Serial Number 119
November and December 2011
Pages 1374-1382

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History

  • Receive Date: 19 March 2011
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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