The Effect of Ultrasound Parameters on the Destruction of 4T1 Cells in the Presence of Gold Nanoparticles

Document Type : Original Article (s)

Authors

1 Associate Professor, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Associate Professor, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 MSc Student, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: The ultrasound parameters such as intensity and time of radiation play an important role in cellular degradation. In this study, the effect of ultrasound radiation parameters on the degradation of 4T1 cells in the presence of gold nanoparticles was investigated.Methods: The 4T1 cells were cultured. The cells were divided into 4 groups including control, gold nanoparticle, ultrasound alone, and ultrasound at the presence of gold nanoparticle. Ultrasound irradiation were performed on the cells at intensity of 0.5, 1.0, and 1.5 W/cm2, for 1, 1.5, and 2 minutes. The viability of cells was determined using MTT assay.Findings: There was a significant difference in cell viability between the ultrasound and gold nanoparticle group and the other groups at intensity of 1.0 and 1.5 W/cm2. Moreover, there was a significant difference between the ultrasound alone and the control groups at intensity of 1.0 and 1.5 W/cm2.Conclusion: Using ultrasound in the presence of gold nanoparticles increase cell death via increasing nuclei cavitation phenomenon.

Keywords

References

  1. Pulaski BA, Ostrand-Rosenberg S. Mouse 4T1 breast tumor model. Curr Protoc Immunol 2001; Chapter 20: Unit.
  2. Diaz-Zaragoza M, Hernandez-Avila R, Ostoa-Saloma P. Recognition of tumor antigens in 4T1 cells by natural IgM from three strains of mice with different susceptibilities to spontaneous breast cancer. Oncol Lett 2017; 13(1): 271-4.
  3. Tang H, Wang CC, Blankschtein D, Langer R. An investigation of the role of cavitation in low-frequency ultrasound-mediated transdermal drug transport. Pharm Res 2002; 19(8): 1160-9.
  4. Sazgarnia A, Shanei A, Shanei MM. Monitoring of transient cavitation induced by ultrasound and intense pulsed light in presence of gold nanoparticles. Ultrason Sonochem 2014; 21(1): 268-74.
  5. Nordberg J, Arner ES. Reactive oxygen species, antioxidants, and the mammalian thioredoxin system. Free Radic Biol Med 2001; 31(11): 1287-312.
  6. Barati AH, Mokhtari -Dizaji M, Mozdarani H, Bathaei SZ, Hassan ZM. Free hydroxyl radical dosimetry by using 1 MHz low level ultrasound waves. Int J Radiat Res 2006; 3(4): 163-9.
  7. Farny CH, Wu T, Holt RG, Murray TW, Roy RA. Nucleating cavitation from laser-illuminated nano-particles. Acoustics Research Letters Online 2005; 6(3): 138-43.
  8. Haosheng C, Jiadao W, Darong C. Cavitation damages on solid surfaces in suspensions containing spherical and irregular microparticles. Wear 2009; 266(1): 345-8.
  9. Tuziuti T, Yasui K, Sivakumar M, Iida Y, Miyoshi N. Correlation between acoustic cavitation noise and yield enhancement of sonochemical reaction by particle addition. J Phys Chem A 2005; 109(21): 4869-72.
  10. Seol Y, Carpenter AE, Perkins TT. Gold nanoparticles: enhanced optical trapping and sensitivity coupled with significant heating. Opt Lett 2006; 31(16): 2429-31.
  11. Frenkel V. Ultrasound mediated delivery of drugs and genes to solid tumors. Adv Drug Deliv Rev 2008; 60(10): 1193-208.
  12. Shanei A, Tavakoli MB, Salehi H, Ebrahimi-Fard A. Evaluating the effects of ultrasound waves on MCF-7 cells in the presence of AG nanoparticles. J Isfahan Med Sch 2016; 34(389): 763-8. [In Persian].
  13. Sazgarnia A, Shanei A, Eshghi H, Hassanzadeh-Khayyat M, Esmaily H, Shanei MM. Detection of sonoluminescence signals in a gel phantom in the presence of Protoporphyrin IX conjugated to gold nanoparticles. Ultrasonics 2013; 53(1): 29-35.
  14. Sazgarnia A, Shanei A, Taheri AR, Meibodi NT, Eshghi H, Attaran N, et al. Therapeutic effects of acoustic cavitation in the presence of gold nanoparticles on a colon tumor model. J Ultrasound Med 2013; 32(3): 475-83.
  15. Ebrahimi Fard A, Zarepour A, Zarrabi A, Shanei A, Salehi H. Synergistic effect of the combination of triethylene-glycol modified Fe3O4 nanoparticles and ultrasound wave on MCF-7 cells. J Magn Magn Mater 2015; 394: 44-9.
  16. Sazgarnia A, Shanei A, Meibodi NT, Eshghi H, Nassirli H. A novel nanosonosensitizer for sonodynamic therapy: in vivo study on a colon tumor model. J Ultrasound Med 2011; 30(10): 1321-9.
Journal of Isfahan Medical School
Volume 36, Issue 479 - Serial Number 479
March and April 2018
Pages 524-529

Files

History

  • Receive Date: 23 June 2018
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

Share

How to cite

Statistics