Effect of Intraperitoneal Injection of Lipopolysaccharide (LPS) on Apoptosis, Memory and Alzheimer’s Disease Processes in the Hippocampus of Male Wistar Rats in a Time-Dependent Manner

Document Type : Original Article (s)

Authors

1 Associate Professor, Department of Animal Physiology, School of Biology, Kharazmi University, Tehran, Iran

2 Department of Animal Physiology, Schoo of Biology, Kharazmi University, Tehran, Iran

3 Associate Professor, Department of Cellular and Molecular Biology, School of Biology, Kharazmi University, Tehran, Iran

4 Assistant Professor, Department of Cellular and Molecular Biology, School of Biology, Kharazmi University, Tehran, Iran

5 Assistant Professor, Department of Biology, School of Science, Lorestan University, Khorramabad, Iran

Abstract

Background: In this study, the effects of intraperitoneal injection of lipopolysaccharide (LPS) in causing apoptosis by increasing the amount of protein apoptotic B-cell lymphoma-2- associated X protein (Bax) and Caspase-3 and decreasing protein anti-apoptotic B-cell lymphoma-2 (bcl-2) in the hippocampus of rats and its effect on memory in a time-dependent manner at 12, 24, 48, 72 and 120 hours after injection were examined.Methods: Male Wistar rats of 230 to 250 g for Western blotting, enzymatic test and shuttle box were used.Findings: Increase in the protein content of apoptotic Bax and Caspase-3 and decrease in antiapoptotic bcl-2 protein in a time-dependent manner was observed in cases compared to the controls. The results of testing the enzyme Caspase-3 confirmed Western blotting results. Dysfunction caused by LPS in memory determined by shuttle box test. All the results were significant at the level of P ˂ 0.001.Conclusion: Results showed that LPS increased the incidence of apoptosis and neuronal cell death in the hippocampus region of the brain of male Wistar rats in a time-dependent manner and decrease animal's memory.

Keywords

References

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Journal of Isfahan Medical School
Volume 32, Issue 285 - Serial Number 285
March and April 2014
Pages 650-659

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History

  • Receive Date: 09 March 2014
  • Revise Date:
  • Accept Date: 06 April 2022

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