مقایسهی غلظت پروتئین ماتریکس متالو پروتئیناز-9 در سرم و پلاسمای بیماران مبتلا به سرطان پستان و ارتباط آن با خصوصیات کلینیکی بیماری

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 استادیار، گروه زیست شناسی بخش ژنتیک، دانشکده‌ی علوم، دانشگاه اصفهان، اصفهان، ایران.

2 استادیار، گروه رادیوتراپی و انکولوژی، دانشکدهی پزشکی‎ دانشگاه علوم پزشکی اصفهان، اصفهان، ایران.

3 کارشناس ارشد ژنتیک مولکولی، گروه زیست شناسی بخش ژنتیک، دانشکده‌ی علوم، دانشگاه اصفهان، اصفهان، ایران

چکیده

مقدمه: ماتریکس متالوپروتئیناز-9 (MMP-9) به تازگی به عنوان یکی از ژن‌های مارکر برای بسیاری از سرطان‌ها گزارش شده است. با توجه به نقش مهم این ژن در تشخیص انواع سرطان‌ها در این مطالعه ما به مقایسه ارتباط غلظت پلاسمایی و سرمی این آنزیم با خصوصیات کلینیکی بیماران مبتلا به سرطان پستان پرداختیم.روش‌ها: در این مطالعه‌ی مورد شاهدی120 بیمار مبتلا به سرطان پستان و90 فرد سالم مورد مطالعه قرار گرفتند. برای بررسی غلظت MMP-9 در سرم و پلاسمای بیماران از روش زیموگرافی ژلاتین و کیت مخصوص استفاده شد و اثر هر یک از غلظت‌های پلاسمایی و سرمی بر وضعیت کلینیکی بیماران بررسی و مقایسه گردید.یافته‌ها: غلظت MMP-9 پلاسمایی در بیماران بسیار بیشتر از افراد سالم بود  (00/0>P). این در حالی بود که غلظت سرمی در دو گروه مشابه بود. به علاوه ارتباط معنی داری بین افزایش غلظت پلاسمایی MMP-9 و متاستاز به غدد لنفاوی (OR, 3.43; P = 0.019) و تهاجم وریدی (OR, 4.14; P = 0.033)   بیماران دیده شد اما هیچ ارتباطی بین غلظت MMP-9 سرمی و متاستاز لنفاوی یا تهاجم وریدی مشاهده نشد.نتیجه‌گیری: طبق یافته های این مطالعه غلظت پلاسمایی MMP-9 در مقایسه‌ی با غلظت سرمی آن فاکتور مناسبی جهت تشخیص زودهنگام و بررسی متاستاز به غدد لنفاوی و تهاجم وریدی در مبتلایان به سرطان پستان است.

کلیدواژه‌ها

عنوان مقاله [English]

Comparison of serum and plasma MMP-9 level in breast cancer patients and its correlation with the clinical features of the patients

نویسندگان [English]

  • Majid Motevali-bashi 1
  • Simin Hemmati 2
  • Morteza Sadeghi 3
1 Assistant Professor, Biology Department, School of Sciences, Isfahan University, Isfahan, Iran.
2 Assistant Professor, Oncology and Radiotherapy Department, Medicine School, Isfahan University of medical sciences, Isfahan, Iran.
3 BS of molecular genetic, Biology Deppartment, School of Sciences, Isfahan University, Isfahan, Iran.
چکیده [English]

Background: Matrix metalloproteinase-9 (MMP-9) has been reported as a new marker gene for several cancers. With respect to the significance function of this gene in diagnosis of cancers in the present study we compare the correlation betweenthe plasma and serum MMP-9 levels and clinical features of breast cancer patients.Methods: In this case-control study, 120breast cancer patients and 87 healthy controls were studded.MMP-9 level was quantitatively measured byzymography and specific kit. The results were compared with clinical features of patients.Finding: Plasma MMP-9 level wassignificantly elevated in breast cancer patients comparedwith the control subjects (P < 0.001). Also there was a correlation between the plasma MMP-9 level and lymph node metastasis (OR, 3.43; P = 0.019) and venous invasion (OR, 4.14; P = 0.033) of the patients. There was no correlation between the serum MMP-9 level and lymphatic metastasis or venous invasion.Conclusion: according to our findings the plasma MMP-9 level  in comparison with the serum MMP-9 level is a better determinant for early diagnosis, lymph node metastasis and venous invasion in patients with breast cancer. 

کلیدواژه‌ها [English]

  • Breast Cancer
  • Matrix mataloproteinase-9
  • Plasma level
  • Serum level

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مجله دانشکده پزشکی اصفهان
دوره 28، شماره 118 - شماره پیاپی 118
بهمن و اسفند 1389
صفحه 1253-1260

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سابقه مقاله

  • تاریخ دریافت: 28 اسفند 1389
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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