اثر جداگانه و همزمان یک دوره‌ی تمرین شنا و مصرف مکمل کورستین بر استئوآرتریت زانوی موش صحرایی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشیار، گروه زیست‌شناسی، دانشکده‌ی علوم پایه، دانشگاه مازندران، بابلسر، ایران

2 دانشجوی دکتری، گروه بهداشت و طب ورزشی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

3 دانشیار، گروه بهداشت و طب ورزشی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

4 دانشیار، گروه فیزیولوژی ورزشی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

5 دانشجوی دکتری، گروه آسیب‌شناسی ورزشی و حرکات اصلاحی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه خوارزمی، تهران، ایران

چکیده

مقدمه: استئوآرتریت (OA یا Osteoarthritis) یکی از شایع‌ترین اختلالات عضلانی- اسکلتی است که می‌تواند به درد مزمن و ناتوانی شدید بیمار منجر شود و پیش‌بینی شده است با افزایش سن جمعیت، شیوع آن در آینده به طور چشمگیری افزایش یابد.روش‌ها: در تحقیق تجربی حاضر، 30 سر موش صحرایی نر نژاد ویستار (میانگین وزن 1 ± 173 گرم و سن 8 هفته) به طور تصادفی به 5 گروه 6تایی شامل گروه‌های شاهد سالم، شاهد پایه، کورستین، شنا و نیز کورستین + شنا تقسیم شدند. برای ایجاد استئوآرتریت از تزریق درون مفصلی، مونو سدیم یدواستات (lµ 50) در زانوی راست نمونه‌ها استفاده شد. پروتکل تمرینی این پژوهش شامل شنا با شدت متوسط و به مدت 28 روز بود. پس از اتمام دوره، حیوانات کشته شدند و مفاصل زانوی هر دو پای آن‌‌ها مورد بررسی هیستوپاتولوژیکی قرار گرفت. از آزمون آماری One-way ANOVA (One-way analysis of variance) (050/0 ≥ P) و سپس آزمون تعقیبی Tukey به منظور تجزیه و تحلیل کمی یافته‌ها استفاده شد.یافته‌ها: در رابطه با شاخص نسبت عمق ضایعه تفاوت معنی‌داری بین گروه کورستین (002/0 = P)، شنا (001/0 = P) و کورستین + شنا (001/0 = P) با گروه شاهد پایه وجود داشت (001/0 = P). همچنین در رابطه با شاخص عرض کل ناحیه‌ی تخریب شده و عرض ناحیه‌ی تخریب شده به طور معنی‌دار، تفاوت معنی‌داری بین تمامی گروه‌ها با گروه شاهد پایه وجود داشت (001/0 = P).نتیجه‌گیری: مصرف مکمل کورستین و تمرین شنا با شدت متوسط، هر یک به تنهایی و به صورت ترکیبی می‌توانند اثر مثبتی در بهبود علایم استئوآرتریت زانوی موش‌های صحرایی داشته باشند. 

کلیدواژه‌ها

عنوان مقاله [English]

Separate and Concomitant Effect of Swimming Exercise and Quercetin Supplementation on Rat’s Knee Osteoarthritis

نویسندگان [English]

  • Akbar Hajizadeh-Moghaddam 1
  • Seyed Hossein Mirkarimpour 2
  • Mohammad Hossein Alizadeh 3
  • Mohammad Reza Kordi 4
  • Mohammad Fallah-Mohammadi 5
1 Associate Professor, Department of Biology, School of Basic Sciences, University of Mazandaran, Babolsar, Iran
2 PhD Student, Department of Sport Medicine, School of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
3 Associate Professor, Department of Sport Medicine, School of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
4 Associate Professor, Department of Sport Physiology, School of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
5 PhD Student, Department of Sport Traumatology, School of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran
چکیده [English]

Background: Osteoarthritis is the most prevalent musculoskeletal disorder leading to chronic pain and severe disability and its prevalence is predicted to raise significantly in the future as the population ages.Methods: Thirty male Wistar rats (weight of 173 ± 1 g, age of 8 weeks) were randomly divided into 5 equal experimental groups: 1. Intact control, 2. Osteoarthritis, 3. Quercetin 4. Swimming, and 5. Quercetin + Swimming. Intra-articular injection of 50 µl of monosodium iodoacetate (MIA) was applied on right knee of rats; similarly, saline was injected in left knees. The used training program was swimming in moderate intensity for 28 days. After the completion of the protocol, rats were killed and both knees of the animals were assessed histopathologically. One-way analysis of variances (ANOVA) and post-hoc Tukey tests were used for statistical analysis, at the significant level of P < 0.05.Findings: Results indicated a significant difference between quercetin, swimming and quervetin + swimming groups with the osteoarthritis group (P = 0.001).Conclusion: Regarding results of the study, it can be concluded that swimming exercise at moderate intensity and quercetin supplementation, either alone or in combination, have a positive effect on improvement of osteoarthritis symptoms of rat’s knee. But, it seems that separate effect of quercetin supplementation and its combination with swimming exercise does not excel the effect of swimming exercise alone.

کلیدواژه‌ها [English]

  • Swimming exercise
  • Quercetin
  • Rat’s knee osteoarthritis

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مجله دانشکده پزشکی اصفهان
دوره 32، شماره 288 - شماره پیاپی 288
مرداد و شهریور 1393
صفحه 805-817

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سابقه مقاله

  • تاریخ دریافت: 13 آبان 1392
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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