القای تشکیل سلول‌های شبه عصبی از سلول‌های بنیادی مشتق از بافت چربی در هیدروژل آلژینات با استفاده از روش تشکیل نوروسفر

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 استاد، گروه علوم تشریح و بیولوژی مولکولی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

2 دانشجوی دکتری، گروه علوم تشریح و بیولوژی مولکولی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی تهران، تهران، ایران

3 دانشیار، گروه علوم تشریح و بیولوژی مولکولی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

4 دانشجوی دکتری، گروه ژنتیک، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

مقدمه: هیدروژل‌ها محیط سه بعدی مناسبی را برای کشت انواع مختلف سلول‌ها فراهم می‌کنند و انکپسوله کردن سلول در هیدروژل‌ها روش مناسبی جهت استفاده در مهندسی بافت است. آلژینات، هیدروژل زیست سازگاری است که سیستم مناسبی برای انکپسوله کردن سلول‌ها فراهم می‌کند. به علاوه، سلول‌های بنیادی جدا شده از بافت چربی، سلول‌های بنیادی مزانشیمی هستند که ممکن است منبع مناسبی از سلول‌ها جهت استفاده در سلول درمانی به صورت اتولوگ باشند.روش‌ها: در این مطالعه سرنوشت سلول‌های بنیادی جدا شده از بافت چربی انکپسوله در هیدروژل آلژینات را که به مدت یک هفته در محیط کشت القای عصبی کشت شدند، بررسی شد. با استفاده از روش MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] و تکنیک ایمنوسیتوشیمی به ترتیب میزان تکثیر و تمایز عصبی این سلول‌ها بررسی شد.یافته‌ها: افزایش معنی‌داری در میانگین درصد سلول‌های GFAP (Glial fibrillary acidic protein)، Nestin و 2MAP (2Microtubule-associated protein) مثبت و کاهش معنی‌داری در میزان تکثیر و بقای سلول‌های انکپسوله در مقایسه با سلول‌های کشت تک لایه مشاهده شد.نتیجه‌گیری: هیدروژل آلژینات می‌تواند محیط مناسبی برای تمایز عصبی سلول‌های بنیادی جدا شده از بافت چربی فراهم کند.

کلیدواژه‌ها

عنوان مقاله [English]

Induction of Neuron-Like Cells from Adipose Derived Stem Cells in Alginate Hydrogel, Using Neurospheres Formation

نویسندگان [English]

  • Shahnaz Razavi 1
  • Zahra Khosravizadeh 2
  • Hamid Bahramian 3
  • Mohammad Kazemi 4
1 Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 PhD Student, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3 Associate Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 PhD Student, Department of Genetics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده [English]

Background: Hydrogels provide appropriate three-dimensional environment for culture of a variety of cells and cell encapsulation in hydrogels is a promise plan for tissue engineering applications. Alginate is an attractive biocompatible hydrogel that provides a supportive system for the encapsulated cells. Moreover, human adipose derived stem cells are mesenchymal stem cells that might be a suitable source of cells for use in autologous cell therapy.Methods: In this study, we examined the fate of human adipose derived stem cells encapsulated in alginate hydrogel that cultured in neural induction medium for 1 week. Using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and immunocytochemical analysis, the proliferation rate, and viability and neural differentiation of human adipose derived stem cells were evaluated.Findings: We observed a significant increase in the mean percent of Nestin, glial fibrillary acidic protein (GFAP) and microtubule-associated protein-2 (MAP2) positive cells and significant reduction of proliferation rate and viability in encapsulated cells versus monolayer induced cells.Conclusion: These findings showed that alginate hydrogel can provide a suitable environment for neural differentiation of human adipose derived stem cells.

کلیدواژه‌ها [English]

  • Hydrogel alginate
  • Human adipose-derived stem cells
  • Neural differentiation
  • Proliferation

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مجله دانشکده پزشکی اصفهان
دوره 32، شماره 288 - شماره پیاپی 288
مرداد و شهریور 1393
صفحه 828-840

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سابقه مقاله

  • تاریخ دریافت: 12 بهمن 1392
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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