بررسی نقش سیستم یوبیکوئیتین- پروتئوزوم در فرایند اسپرماتوژنز

نوع مقاله : مقاله مروری

نویسندگان

1 کارشناس ارشد، پژوهشگاه رویان، پژوهشکده‌ی زیست فناوری جهاد دانشگاهی، گروه زیست فناوری تولید مثل، مرکز تحقیقات پزشکی تولید مثل، اصفهان، ایران

2 دانشجوی دکتری، گروه زیست‌شناسی تکوین و عضو هیأت علمی، پژوهشکده‌ی زیست فناوری جهاد دانشگاهی، پژوهشگاه رویان و گروه زیست فناوری تولید مثل، مرکز تحقیقات پزشکی تولید مثل، اصفهان، ایران

3 استاد، پژوهشکده‌ی زیست فناوری جهاد دانشگاهی، پژوهشگاه رویان و گروه زیست فناوری تولید مثل، مرکز تحقیقات پزشکی تولید مثل و مرکز باروری و ناباروری اصفهان، اصفهان، ایران

چکیده

مقدمه: یوبیکوئیتین اولین بار در سال 1975 به عنوان یک پروتئین kDa-5/8 موجود در سلول شناسایی شد. یوبیکوئیتین، یک پروتئین بسیار حفاظت شده است؛ به طوری که یوبیکوئیتین انسان و مخمر در 96 درصد توالی ژنتیکی مشترک هستند. این پپتید، به طور معمول به عنوان شاخص نشان‌گذاری سلول جهت حذف توسط پروتئوزوم شناخته شده است، اما نقش‌های دیگری همانند دخالت در آپوپتوز، تمایز و غیره برای آن در نظر گرفته شده است. آن چه که بیشتر در این مطالعه مد نظر می‌باشد، بررسی نقش سیستم یوبیکوئیتین- پروتئوزوم در فرایند اسپرماتوژنز است.روش‌ها: مقالات جستجو شده در پایگاه‌های اطلاعاتی Pubmed Entrez و پایگاه‌های مرتبط با مقالات ISI مورد بررسی قرار گرفتند.یافته‌ها: یوبیکوئیتین در طی بلوغ اسپرم، از سلول‌های اپیتلیال اپیدیدیم آزاد می‌شود و بر روی سطح اسپرم‌های غیر طبیعی که باید حذف شوند، قرار می‌گیرد. امکان دارد تعدادی از این اسپرم‌ها بدون حذف شدن در اپیدیدیم، در مایع انزال مشاهده شود.نتیجه‌گیری: در برخی از تحقیقات دیده شده است که یوبیکوئیتیناسیون ممکن است به عنوان یک روند فیزیولوژیک در طی ظرفیت‌یابی افزایش پیدا کند و این افزایش، نشانه‌ی کیفیت خوب اسپرم است. بنابراین، یوبیکوئیتیناسیون علاوه بر حذف اسپرم‌های آسیب دیده، می‌تواند در فرایند ظرفیت‌یابی اسپرم نیز نقش داشته باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Role of Ubiquitin-Proteasome in Spermatogenesis Process

نویسندگان [English]

  • Erfaneh Shaygannia 1
  • Marziyeh Tavalaei 2
  • Mohammad Hossein Nasr-Esfahani 3
1 Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, The Academic Center for Education, Culture and Research (ACECR), Isfahan, Iran
2 PhD Student, Faculty Member, Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, The Academic Center for Education, Culture and Research (ACECR), Isfahan, Iran
3 Professor, Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, The Academic Center for Education, Culture and Research (ACECR) AND Isfahan Fertility and Infertility Center, Isfahan, Iran
چکیده [English]

Background: Ubiquitin first time was known at 1975, as an 8.5 KDa protein, existing in the cell. Ubiquitin protein has been highly preserved and it has 96% homology gene sequencing between human and yeast. This peptide has been identified as an index of cell mark for removing it via “proteasome”. But, other functions such as participating in the apoptosis, differentiation and etc. have been considered for ubiquitination. In the present study, we tried to concentrate on role of ubiquitin-proteasome system in spermatogenesis process.Methods: Literature search in Entrez PubMed database, as well as other data sources related to ISI Web of Knowledge.Findings: Ubiquitin is secreted through epithelial cells of epididymis during sperm maturation, and placed on surface of abnormal sperm that is deleted. It is possible that some of this sperms escape from deletion in epididymis and appear in ejaculate.Conclusion: Some researches indicated that ubiquitination may increase as a physiological phenomenon during the sperm capacitation which is a positive marker to confirm sperm quality. Therefore, addition to role of ubiquitination in removing abnormal sperm, it can be involved in sperm capacitation process.

کلیدواژه‌ها [English]

  • Ubiquitination
  • Ubiquitin-proteasome pathway
  • Spermatogenesis
  • Male infertility

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مجله دانشکده پزشکی اصفهان
دوره 32، شماره 288 - شماره پیاپی 288
مرداد و شهریور 1393
صفحه 854-870

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  • تاریخ دریافت: 29 مرداد 1392
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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