The Effect of Ultrasound Therapy in the Presence of Combined Zinc Oxide/Gold Nanocomposite on Melanoma Cell Line

Document Type : Original Article (s)

Authors

1 PhD in Medical Physics, Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

2 Professor, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Assistant Professor, Department of Medical Nanotechnology, Applied Biophotonics Research Center, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran

4 Assistant Professor, Department of Biomedical Engineering, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran

5 Msc in Biomedical Engineering, Department of Biomedical Engineering, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran

6 Professor, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Zinc oxide nanoparticles can produce free radicals due to their piezoelectric properties, which are needed for cancer treatment. Also, using ultrasound waves in combination with zinc oxide nanoparticles can help increase the response to sonodynamic therapy by synergistically producing free radicals. On the other hand, with gold nanoparticles as a place for cavitation in the ultrasound field, the intensity threshold required for cavitation is reduced, reducing the damage to normal cells. This study aimed to investigate the effect of sonodynamic therapy of melanoma with zinc oxide/gold nanocomposite as a sonosensitizer.
Methods: Chemical dosimetry was used to determine the level of free radicals produced due to combined zinc oxide/gold nanocomposite with and without ultrasound radiation. Also, measuring the survival and apoptosis rate in the cultured melanoma cells was done in-vitro by MTT and flow cytometry tests, respectively.
Findings: With the increase in the production of free radicals, the survival percentage of melanoma cells treated with combined zinc oxide/gold nanocomposite and ultrasound waves at an intensity of 2 W/cm2 showed the most significant decrease (by 20%) which was significantly different from the control group. Also, the apoptosis level caused by sonodynamic therapy with zinc oxide/gold nanocomposite increased significantly.
Conclusion: Using ultrasound waves in combination with zinc oxide/gold nanocomposite can play an influential role in improving the response of melanoma to treatment by raising the level of free radicals and reducing the general toxicity for non-cancerous cells by lowering the cavitation threshold.

Keywords

References

  1. Siegel R, Miller KD, Jemal A. Cancer statistics, 2019. CA Cancer J Clin 2019; 69(1):7-34.
  2. Domingues B, Lopes JM, Soares P, Pópulo H. Melanoma treatment in review. Immunotargets Ther 2018; 7: 35-49.
  3. Bansal K, Jha CK, Bhatia D, Shekhar H. Ultrasound-enabled therapeutic delivery and regenerative medicine: Physical and biological perspectives. ACS Biomater Sci Eng 2021; 7(9): 4371-87.
  4. Shanei A, Baradaran M, Shanei MM. The effect of ultrasound waves on melanoma cells in presence of gold nanoparticles [in Persian]. J Isfahan Med Sch 2017; 34(412): 1550-5.
  5. Cai L, Liu J, Wang Y, Chen H, Ma Y, Wang Y, et al. Enhanced anti-melanoma efficacy of interferon α-2b via overexpression of ING4 by enhanced Fas/FasL-mediated apoptosis. Oncol Lett 2018; 15(6): 9577-83.
  6. Pan X, Wang H, Wang S, Sun X, Wang L, Wang W, et al. Sonodynamic therapy (SDT): a novel strategy for cancer nanotheranostics. Sci China Life Sci 2018; 61(4): 415-26.
  7. Chen X, Niu T, Gao Y, Liang X, Li S, Zhang L, et al.
    Tunable synthesis of pH-responsive biodegradable ZnO nanospheres assembled from ultrasmall particles for cancer chemotherapy. Chem Eng J 2019; 371: 443-51.
  8. Liu Y, Wang Y, Zhen W, Wang Y, Zhang S, Zhao Y, et al. Defect modified zinc oxide with augmenting sonodynamic reactive oxygen species generation. Biomaterials 2020; 251: 120075.
  9. Duan X, Liao Y, Liu T, Yang H, Liu Y, Chen Y,
    et al. Zinc oxide nanoparticles synthesized from Cardiospermum halicacabum and its anticancer activity in human melanoma cells (A375) through the modulation of apoptosis pathway. J Photochem Photobiol B 2020; 202: 111718.
  10. Fan P, Yang C, Wang L, Wang Q, Zhang Y, Zhou J, et al. ZnO nanoparticles stimulate oxidative stress to induce apoptosis of B16F10 melanoma cells: In vitroandin vivostudies. Biomed Phys Eng Express 2021; 7(6).
  11. Venkatasubbu G, Baskar R, Anusuya T, Seshan CA, Chelliah R. Toxicity mechanism of titanium dioxide and zinc oxide nanoparticles against food pathogens. Colloids Surf B Biointerfaces 2016; 148: 600-6.
Journal of Isfahan Medical School
Volume 40, Issue 695
3rd Week, January
January and February 2023
Pages 917-923

Files

History

  • Receive Date: 28 July 1401
  • Revise Date: 24 October 1401
  • Accept Date: 19 September 1401

Share

How to cite

Statistics