Study and Detection of Ultra-Weak Photon Emission from HT-29 Cells under the Effect of Trypsin

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Medical Physics, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

2 Associate Professor, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Associate Professor, Department of Physics, School of Physics, Isfahan University of Technology, Isfahan, Iran

4 Assistant Professor, Department of Health Physics, Applied Sciences Complex, Maleke-Ashtar University of Technology, Isfahan, Iran

Abstract

Background: In addition to chemical and electrical mechanisms, cell organisms communicate each other through electromagnetic waves, known as biophotons. Detection of these waves shows that when cell organisms are being dividing, injured, or dying, they emit more photons than when they are healthy. The purpose of this study was to detect the biophoton waves emitted from cancer cells (HT-29) affected by trypsin. Trypsin is a factor contributes to gradual death of cells.Methods: This study was carried out on HT-29 cells in three groups. In the first group, the cells were affected by trypsin. In the second group, the cells were not influenced by trypsin; and in the third group, an empty plate was used to measure the background radiation. For this purpose, the amount of the radiation emitted from all three groups was measured using photomultiplier tubes (PMT) apparatus and compared.Findings: Computation of the means and standard deviation of the whole surface of emitted biophotons demonstrated that the group containing trypsin significantly emitted more amounts of biophotons than the group without trypsin and the group considered for the measurement of the background radiation (P < 0.05). In addition, over the time, the amount of emitted biophotons increased.Conclusion: The results showed that when the cells were under the influence of trypsin, the amount of biophoton emissions increased. With the elapsed time, more cells were faced with the death threshold, therefore, the amount of emitted waves from the cells affected by trypsin increased significantly rather than the cells were not influenced by trypsin.

Keywords

References

  1. Scholkmann F, Fels D, Cifra M. Non-chemical and non-contact cell-to-cell communication: a short review. Am J Transl Res 2013; 5(6): 586-93.
  2. Trushin MV. Studies on distant regulation of bacterial growth and light emission. Microbiology 2003; 149(Pt 2): 363-8.
  3. Lorenz E. Search for mitogenetic radiation by means of the photoelectric method. J Gen Physiol 1934; 17(6): 843-62.
  4. Rossi C, Foletti A, Magnani A, Lamponi S. New perspectives in cell communication: Bioelectromagnetic interactions. Semin Cancer Biol 2011; 21(3): 207-14.
  5. Bischof M. Synchronization and coherence as an organizing principle in the organism, social interaction, and consciousness. NeuroQuantology 2008; 6(4): 440-51.
  6. Rattemeyer M, Popp FA, Nagl W. Evidence of photon emission from DNA in living systems. Naturwissenschaften 1981; 68(11): 572-3.
  7. Popp FA, Nagl W, Li KH, Scholz W, Weingartner O, Wolf R. Biophoton emission. New evidence for coherence and DNA as source. Cell Biophys 1984; 6(1): 33-52.
  8. Gurwitsch AG, Gurwitsch LG. Die mitogenetische strahlung. Jena, Germany: Gustav Fischer Verlag; 1959. [In Germany].
  9. Vanwijk R. Bio-photons and bio-communication. J Sci Explor 2001; 15(2): 183-97.
  10. Bateman JB. Mitogenic radiation. Biological Review 1935; 10(1): 42-71.
  11. Rastogi A, Pospisil P. Effect of exogenous hydrogen peroxide on biophoton emission from radish root cells. Plant Physiol Biochem 2010; 48(2-3): 117-23.
  12. Schauf B, Repas L, Kaufmann R. Localization of ultra weak photon emission in plants. Photochem Photobiol 1992; 55(2): 287-91.
  13. Hideg E. On the spontaneous ultraweak light emission of plants. J Photochem Photobiol B Biol 1993; 18(2-3): 239-44.
  14. Yoshinagato N, Kato K, Kageyama Ch, Fujisaki K, Nishida R, Mori N. Ultraweak photon emission from herbivory-injured maize plants. Naturwissenschaften 2006; 93(1): 38-41.
  15. Cheun BS, Yi SH, Baik KY, Lim JK, Yoo JS, Shin HW, et al. Biophoton emission of MDCK cell with hydrogen peroxide and 60 Hz AC magnetic field. J Environ Biol 2007; 28(4): 735-40.
  16. Zamanian Azodi M, Rezaei Tavirani K, Mousavi M. Comparison between physical and enzymatic harvesting methods via flow cytometry. Behbood J 2013; 16(8): 644-9. [In Persian].
  17. Bischof M. Biophotons - the light in our cells. International Lights Association Website [Online]. [cited 2005 Mar]; Available from: URL:
  18. http://www.bibliotecapleyades.net/ciencia/ciencia_fuerzasuniverso06.htm
  19. Yu Z, Li W, Liu F. Inhibition of proliferation and induction of apoptosis by genistein in colon cancer HT-29 cells. Cancer Lett 2004; 215(2): 159-66.
  20. Gertler R, Rosenberg R, Schuster T, Friess H. Defining a high-risk subgroup with colon cancer stages I and II for possible adjuvant therapy. Eur J Cancer 2009; 45(17): 2992-9.
  21. Ren MR, Hur JS, Kim JY, Park KW, Park SC, Seong CN, et al. Anti-proliferative effects of Lethariella zahlbruckneri extracts in human HT-29 human colon cancer cells. Food Chem Toxicol 2009; 47(9): 2157-62.
  22. Krause S, Maffini MV, Soto AM, Sonnenschein C. The microenvironment determines the breast cancer cells' phenotype: organization of MCF7 cells in 3D cultures. BMC Cancer 2010; 10: 263.
  23. Soto AM, Sonnenschein C. The somatic mutation theory of cancer: growing problems with the paradigm? Bioessays 2004; 26(10): 1097-107.
Journal of Isfahan Medical School
Volume 33, Issue 364 - Serial Number 364
November and December 2016
Pages 2219-2224

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History

  • Receive Date: 22 June 2015
  • Revise Date: 14 April 2022
  • Accept Date: 06 April 2022

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