Allelic Frequency of Two Polymorphisms (rs1815739) and (rs2228570) in Alpha-actinin3 and Vitamin D Absorption Receptor Genes in Athletes Population

Document Type : Original Article (s)

Authors

1 MSc in Genetics, Department of Biology, School of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Professor, Department of Biology, School of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Assistance Professor, Department of Sport Physiology, School of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Assistant Professor, Department of Biology, School of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

5 MSc, Department of Sport Physiology, School of Sport Sciences, Shahid chamran university of Ahvaz, Ahvaz, Iran

Abstract

Background: Today, by determining the genotype, genetic science can check in which sport a person can perform more successfully.In this study, two important polymorphisms; one is ACTN3, which plays an essential role in the formation of the diversity of muscle fibers, and the other is VDR, which plays a vital role in the growth of muscle fibers and bones, were investigated.
Methods: In this study, 200 male and female professional athletes, with normal BMI and at least five years of previous professional sports, in the age range of 20-35 years with a peak power score of 1000-1100 W in the male population; and Peak power: 700-750 W in the female population by examining the Wingate test, and the minimum muscle percentage of SMM: 30% were selected according to the body composition machine analyzes and compared with the control group of 200 people. After sampling and DNA extraction, polymorphisms were analyzed using the RFLP-PCR technique analysis software.
Findings: The results of the present study showed that there is a significant difference between the muscle mass indices and the Wingate test score between the male and female populations in the ratio of control samples, also significant differences were observed for the rs1815739 polymorphism in the heterozygous state and the rs2228570 polymorphism in the homozygous form.
Conclusion: It seems that in the polymorphism of rs1815739, athletes need both types of fast and slow twitch muscle fibers for their success, and moreover the existence of more homozygous GG in the polymorphism of rs2228570 has helped athletes absorb vitamin D better.

Keywords

Main Subjects

References

  1. D'Isanto T, Raiola G, Altavilla G, D’Elia F. Assessment of sport performance: Theoretical aspects and practical indications. Sport Mont J 2019: 17: 79-82.
  2. Hodkinson P. Youth cultures and the rest of life: Subcultures, post-subcultures, and beyond. Journal of Youth Studies 2016; 19(5): 629-45.
  3. Sutula V. General definition of the concept of sports. J Phy Fit Treatment & Sport 2018; 4(4): 1-2.
  4. Madigan D, Gustafsson H, Smith A, Raedeke T. The BASES expert statement on burnout in sport. Sport and Exercise Scientist 2019; 61: 6-7.
  5. Goodger K, Lavallee D, Harwood C. Burnout in sport: A systematic review. The Sport Psychologist 2007; 21(2): 127-51.
  6. Nazari R, Tabrshi S. The challenge of Iran's Sports development with non-systematic talent identification approach and elite approach. Sport Management and Development 2019; 8(1): 1-14.
  7. Utkualp N, Ercan I. Anthropometric measurements usage in medical sciences. Biomed Res Int 2015: 404261.
  8. Tomporowski PD, McCullick B, Pendleton DM, Pesce C. Exercise and children’s cognition: The role of exercise characteristics and a place for metacognition. Journal of Sport and Health Science 2014; 4(1): 47-55.
  9. Chtourou H, Zarrouk N, Chaouachi A, Dogui M, Behm DG, Chamari K, et al. Diurnal variation in Wingate-test performance and associated electromyographic parameters. Chronobiol Int 2011; 28(8): 706-13.
  10. Jaafar H, Rouis M, Coudrat L, Attiogbé E, Vandewalle H, Driss T. Effects of load on Wingate test performances and reliability. J Strength Cond Res 2014; 28(12): 3462-8.
  11. Varley I, Patel S, Williams AG, Hennis PJ. The current use, and opinions of elite athletes and support staff about genetic testing in elite sports within the UK. Biol Sport 2018; 35(1): 13-9.
  12. McNamee MJ, Müller A, van Hilvoorde I, Holm S. Genetic testing and sports medicine ethics. Sports Med 2009; 39(5): 339-44.
  13. Roth SM. Critical overview of applications of genetic testing in sport talent identification. Recent Pat DNA Gene Seq 2012; 6(3): 247-55.
  14. Jacob YI, Spiteri T, Hart NH, Anderton RS. The potential role of genetic markers in talent identification and athlete assessment in elite sport. Sports (Basel) 2018: 6(3): 88.
  15. Vlahovich N, Fricker PA, Brown MA, Hughes D. Ethics of genetic testing and research in sport: a position statement from the Australian Institute of Sport. Br J Sports Med 2017; 51(1): 5-11.
  16. Bouchard C. Overcoming barriers to progress in exercise genomics. Exerc Sport Sci Rev 2011; 39(4): 212-7.
  17. Vincent B, De Bock K, Ramaekers M, van den Eede E, van Leemputte M, Hespel P, et al. ACTN3 (R577X) genotype is associated with fiber type distribution. Physiol Genomics 2007; 32(1): 58-63.
  18. Coelho DB, Pimenta EM, Rosse IC, Veneroso C, Pussieldi GDA, Becker LK, et al. Alpha-actinin-3 R577X polymorphism influences muscle damage and hormonal responses after a soccer game. J Strength Cond Res 2019; 33(10): 2655-64.
  19. Cauci S, Migliozzi F, Trombetta CS, Venuto I, Saccheri P, Travan L, et al. Low back pain and FokI (rs2228570) polymorphism of vitamin D receptor in athletes. BMC Sports Sci Med Rehabil 2017; 9: 4.
  20. Ozdogan S, Yaltirik CK, Yilmaz SG, Kocak A, Isbir T. Association of rs2228570 polymorphism of vitamin D receptor gene with lumbar degenerative disc disease. Turk Neurosurg 2019; 29(2): 159-63.
  21. Salimi S, Eskandari F, Rezaei M, Sandoughi M. Vitamin D receptor rs2228570 and rs731236 polymorphisms are susceptible factors for systemic lupus erythematosus. Adv Biomed Res 2019; 8: 48.
  22. Webborn N Williams A, McNamee M, Bouchard C, Pitsiladis Y, Ahmetov I et al. Direct-to-consumer genetic testing for predicting sports performance and talent identification: ConsA consensustement. Br J Sports Med 2015; 49(23): 1486-91.
  23. Akazawa N, Ohiwa N, Shimizu K, Suzuki N, Kumagai H, Fuku N, et al. The association of ACTN3 R577X polymorphism with sports specificity in Japanese elite athletes. Biol Sport 2022; 39(4): 905-11.
  24. Abián-Vicén J, Abián P, Bravo-Sánchez A, Piñas-Bonilla I, Lara B, Del Coso J. Genotype Distribution of the ACTN3 p. R577X Polymorphism in Elite Badminton Players: A Preliminary Study. Genes (Basel) 2022; 14(1): 50.
  25. Mohd Fazli NE, Raja Azidin RM, Teh LK, Salleh MZ. Correlations between sports-related polygenic profiles, postural stability, power and strength performances of elite football players. Sport Sci Health 2022; 18(1): 147-54.
  26. Dionísio TJ, Thiengo CR, Brozoski DT, Dionísio EJ, Talamoni GA, Silva RB, Garlet GP, Santos CF, Amaral SL. The influence of genetic polymorphisms on performance and cardiac and hemodynamic parameters among Brazilian soccer players. Appl Physiol Nutr Metab 2017; 42(6): 596-604.
  27. Carswell AT, Jackson S, Swinton P, O'Leary TJ, Tang JC, Oliver SJ, et al. Vitamin D metabolites are associated with physical performance in young healthy adults. Med Sci Sports Exerc 2022; 54(11): 1982-9.
  28. Glocke M, Lang F, Schaeffeler E, Lang T, Schwab M, Lang UE. Impact of vitamin D receptor VDR rs2228570 polymorphism in oldest old. Kidney Blood Press Res 2013; 37(4-5): 311-22.
  29. Ozdogan S, Yaltirik CK, Yilmaz SG, Kocak A, Isbir T. Association of rs2228570 polymorphism of vitamin D receptor gene with lumbar degenerative disc disease. Turk Neurosurg 2019; 29(2): 159-63.
  30. Salimi S, Eskandari F, Rezaei M, Sandoughi M. Vitamin D receptor rs2228570 and rs731236 polymorphisms are susceptible factors for systemic lupus erythematosus. Adv Biomed Res 2019;
    8: 48.
  31. Balberova OV. Candidate genes and single-nucleotide gene variants associated with muscle and tendon injuries in cyclic sports athletes. Personalized Psychiatry and Neurology 2021; 1(1): 64-72.
Journal of Isfahan Medical School
Volume 41, Issue 733
1st Week, November
November and December 2023
Pages 743-750

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History

  • Receive Date: 17 July 2023
  • Revise Date: 20 September 2023
  • Accept Date: 21 September 2023

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