تأثیر تمرین مقاومتی فزاینده و تناوبی شدید بر بیان ژن عامل هسته‌ای کاپا B عضله‌ی قلبی و عامل نکروز دهنده‌ی تومور آلفا سرمی، در موش‌های صحرایی مبتلا به دیابت نوع 2

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشکده‌ی تربیت ‌بدنی و علوم ورزشی، دانشگاه خوارزمی، تهران، ایران

2 استادیار، گروه فیزیولوژی ورزشی، دانشکده‌ی تربیت ‌بدنی و علوم ورزشی، دانشگاه خوارزمی، تهران، ایران

3 استادیار، گروه علوم گیاهی و بیوتکنولوژی، دانشکده‌ی علوم زیستی و منابع طبیعی، دانشگاه شهید بهشتی، تهران، ایران

4 استاد، گروه فیزیولوژی ورزشی، دانشکده‌ی تربیت ‌بدنی و علوم ورزشی، دانشگاه خوارزمی، تهران، ایران

چکیده

مقدمه: شایع‌ترین علل مرگ ‌و میر بیماران مبتلا به دیابت، اختلالات قلبی- عروقی است که یکی از دلایل آن، عوامل التهابی می‌باشد. با توجه به این که فعالیت بدنی قادر به کاهش التهاب است، مطالعه‌ی حاضر، با هدف بررسی تأثیر دو نوع تمرین مقاومتی فزاینده و تمرین تناوبی شدید (High-intensity interval training یا HIIT) بر بیان ژن Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) عضله‌ی قلبی و Tumor necrosis factor-alpha (TNF-α) سرمی موش‌های صحرایی مبتلا به دیابت نر انجام شد.روش‌ها: در این تحقیق تجربی، 72 سر Rat نر نژاد Wistar 6 هفته‌ای به 6 گروه 12تایی شامل موش‌های صحرایی مبتلا به دیابت (12 = n)، شاهد (12 = n)، تمرین تناوبی شدید (High-intensity interval training یا HIIT) (12 = n)، مبتلا به دیابت با تمرین تناوبی شدید (Diabetic + intensity interval training یا DIIT) (12 = n)، مبتلا به دیابت با تمرین مقاومتی شدید (Diabetic + resistance training یا DRT) (12 = n) و تمرین مقاومتی (Resistance training یا RT) (12 = n) تقسیم شدند. تمرین RT در 6 هفته‌ی 3 جلسه‌ای بالا رفتن از نردبان عمودی، همراه با وزنه‌های 50، 75، 90 و 100 درصد وزن بدن حیوان، انجام شد. سپس، 30 گرم به وزنه‌ها اضافه می‌شد، تا جایی که Rat‌ها نتوانند نردبان را طی کنند. HIIT نیز در 6 هفته‌ی 3 جلسه‌ای، با شدت 110-50 درصد بیشینه‌ی اکسیژن مصرفی صورت گرفت. 24 ساعت بعد از اتمام تمرین آزمون عملکردی، قدرت مچ دست و زمان رسیدن به درماندگی سنجش ‌شد و 48 ساعت بعد از آزمون، حیوانات تشریح شدند. در نهایت، بیان ژن NF-κB با استفاده از روش Real-time polymerase chain reaction (Real-time PCR)، مقدار سطوح TNF-α سرمی با استفاده از روش Enzyme-linked immunosorbent assay (ELISA) و آزمون ANOVA مورد ارزیابی قرار گرفت.یافته‌ها: میزان بیان ژن NF-κB در گروه HIIT نسبت به گروه RT افزایش بیشتری داشت (0007/0 = P) و میزان سرمی TNF-α پس از هر دو نوع تمرین کاهش معنی‌داری داشت که در گروه HIIT (13/6) بارزتر بود.نتیجه‌گیری: 6 هفته تمرین HIIT و RT، می‌تواند نقش مهمی در کاهش معنی‌دار التهاب و سازگاری‌های مربوط به قدرت و استقامت داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Progressive Resistance Training and High-Intensity Interval Training on Cardiac Nuclear Factor-Kappa B Gene Expression and Serum Tumor Necrosis Factor-Alpha (TNF-α) in Male Diabetic Rats

نویسندگان [English]

  • Afsaneh Elahi 1
  • Neda Khaledi 2
  • Pejhman Motamedi 2
  • Hossein Askari 3
  • Hamid Rajabi 4
1 PhD Student, Department of Exercise Physiology, School of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran
2 Assistant Professor, Department of Exercise Physiology, School of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran
3 Assistant Professor, Department of Plant Sciences and Biotechnology, School of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
4 Professor, Department of Exercise Physiology, School of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran
چکیده [English]

Background: The most common causes of mortality in patients with diabetes mellitus are cardiovascular disorders; and one of their reasons is inflammatory factors. Given that physical activity can reduce inflammation, the present study aimed to investigate the effects of two types of progressive resistance training (RT) and high-intensity interval training (HIIT) on the expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) gene and serum levels of tumor necrosis factor-alpha (TNF-α) in male diabetic rats.Methods: In this experimental study, 72 male rats were divided into 6 groups of 12 diabetic rats, control, diabetic + intensity interval training (DIIT), HIIT, diabetic + resistance training (DRT), and RT. Progressive resistance training was performed in 6 week, 3 sessions per week, as climbing a vertical ladder with additional weights of 50%, 75%, 90%, and 100% the body weight of the animals. After successful completion, 30 grams were added to the weights, to the extent that the rats could not carry the ladder. HIIT were also performed at in 6 week, 3 sessions per week, with an intensity of 50% to 110% of the VO2max. 24 hours after the completion of the training, the hand grip and time to exhaustion functional test was taken, and the animals were autopsied 48 hours after the test. Finally, the expression of NF-κB gene was evaluated using real-time polymerase chain reaction (real-time PCR) technique and ANOVA statistical test.Findings: The expression of NF-κB gene in HIIT group increased more than RT group (P = 0.0007), and serum TNF-α decreased significantly after both types of exercise in HIIT group (6/13) which was more obvious.Conclusion: 6 weeks of HIIT and RT training can play an important role in significantly reducing inflammation, and adapting to strength and endurance.

کلیدواژه‌ها [English]

  • NF-kappa B
  • TNF-alpha
  • Resistance Training
  • High-Intensity Interval Training
  • Heart
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