تأثیر تمرین هوازی و مصرف مکمل سه گیاه بر موش‌های نر در حال ترک مورفین: بررسی تغییرات در بیان ژن‌های فاکتورهای التهابی و POMC

نوع مقاله : Original Article(s)

نویسندگان

1 دانشجوی دکتری، گروه فیزولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه آزاد اسلامی، واحد اصفهان (خوراسگان)، اصفهان، ایران

2 دانشیار، گروه فیزولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه آزاد اسلامی، واحد اصفهان (خوراسگان)، اصفهان، ایران

10.48305/jims.v42.i767.0377

چکیده

مقاله پژوهشی




مقدمه: این مطالعه با هدف بررسی تأثیر تمرین هوازی و مصرف سه گیاه (زرشک، بابونه، گل نسترن) بر تغییرات فاکتورهای التهابی TNF-a، IL-1b و بیان ژن POMC در مغز موش‌های C57BL/6 در حال ترک از مورفین انجام شد.
روش‌ها: برای این منظور، 54 سر موش C57BL/6 نر به‌طور تصادفی به نه گروه تقسیم‌بندی شدند. برای ایجاد وابستگی، مورفین به آب با ساکارز در غلظت‌های 0/1، 0/2، 0/3 و 0/4 اضافه شد و علائم اعتیاد بررسی شد. مداخلات تمرین هوازی و مصرف سه گیاه به مدت 4 هفته انجام شد. برای ارزیابی رفتاری از آزمون میدان باز و برای  بررسی عوامل التهابی و بیان ژن، از روش Real-time PCR استفاده شد.
یافته‌ها: اعتیاد به مورفین باعث افزایش بیان عوامل التهابی، کاهش بیان ژن‌ POMC و زمان فعالیت حرکتی در موش‌ها شد (0/0001 > P). در گروه معتاد در حال ترک، متادون، مکمل سه گیاه و تمرین هوازی میزان عوامل التهابی نسبت به گروه شاهد سالم افزایش معنی‌داری نداشت ( 0/05 < P)، همچنین کاهش معنی‌داری در میزان POMC و زمان فعالیت حرکتی این گروه مشاهده نشد ( 0/05 < P). مصرف متادون، مکمل سه گیاه و تمرین هوازی و ترکیب هر سه بیان عوامل التهابیTNF-α ،IL-1β را کاهش داد و سطح بیان ژن POMC را در مغز موش‌ها افزایش داد (0/0001 > P)، مکمل سه گیاه، تمرین هوازی و ترکیب مصرف متادون، مکمل سه گیاه، تمرین هوازی میزان زمان فعالیت حرکتی را افزایش داد (0/0001 > P).
نتیجه‌گیری: تمرین هوازی و مصرف سه گیاه، می‌تواند در کاهش علائم اعتیاد، کاهش فاکتورهای التهابی و بهبود عملکرد سیستم عصبی مرکزی مؤثر باشد.

تازه های تحقیق

محبوبه زیبنده پور: Google Scholar, PubMed

فرزانه تقیان: Google Scholar, PubMed

خسرو جلالی دهکردی: Google Scholar, PubMed

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Aerobic Training and Supplementation of Three Herbs on Male Mice in Withdrawal from Morphine: Investigating Changes in

نویسندگان [English]

  • Mahboubeh Zibandehpour 1
  • Farzaneh Taghian 2
  • Khosro Jalali 2
1 PhD Student, Department of Sports Physiology, School of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
2 Associate Professor, Department of Sports Physiology, School of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
چکیده [English]

Background: This study was conducted to investigate the effect of aerobic training and consumption of three herbs (barberry, chamomile, nasturtium) on changes in inflammatory factors (TNF-a, IL-1b) and POMC gene expression in the brain of C57BL/6 mice withdrawing from morphine.
Methods: For this purpose, 54 male C57BL/6 mice were randomly divided into nine groups. Morphine was added to water with sucrose in concentrations of 0.1, 0.2, 0.3, and 0.4 to create dependency, and the symptoms of addiction were investigated. The interventions of aerobic training and consumption of three plants were carried out for four weeks. The real-time PCR method was used for behavioral evaluation of open field tests and investigation of inflammatory factors and gene expression.
Findings: Morphine addiction increased the expression of inflammatory factors and decreased POMC gene expression and motor activity time in rats (P < 0.0001). In the group of drug addicts who are withdrawing methadone, a supplement of three plants, and aerobic exercise, the number of inflammatory factors did not increase in addiction compared to the healthy control group (P > 0.05). There was no decrease in addiction in the amount of POMC and motor activity time of this group (P > 0.05). Methadone consumption, the supplement of three plants and aerobic exercise, and the combination of all three decreased the expression of inflammatory factors TNF-α and IL-1β and increased the expression level of POMC genes in the brain of rats (P < 0.0001), the supplement of three plants, Aerobic exercise and the combination of methadone consumption, supplement of three herbs, aerobic exercise increased the amount of motor activity time (P < 0.0001).
Conclusion: Aerobic training and consumption of three herbs can be effective in reducing addiction symptoms, reducing inflammatory factors, and improving central nervous system function.

کلیدواژه‌ها [English]

  • Addiction
  • Aerobic training
  • Berberis
  • Matricaria chamomilla
  • Rosa canina

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مجله دانشکده پزشکی اصفهان
دوره 42، شماره 767
هفته 3 تیر
خرداد و تیر 1403
صفحه 377-387

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سابقه مقاله

  • تاریخ دریافت: 17 تیر 1403
  • تاریخ پذیرش: 20 تیر 1403

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