The Effects of Intracerebro-Ventricular Administration of Salmon Calcitonin on Sperm Parameters in Rats

Document Type : Original Article (s)

Authors

1 Assistant Professor, Department of Basic Sciences, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Associate Professor, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

3 PhD of Physiology, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

4 Professor, Department of Clinical Science, School of Veterinary Science, Shiraz University, Shiraz, Iran

Abstract

Background: The function of the hypothalamus-pituitary-gonadal axis plays an important role in regulating fertility. This study aimed to investigate the effect of intracerebroventricular (ICV) injection of salmon calcitonin (sCT) on sex hormones and sperm quality in rats.
Methods: This experimental study was conducted in 2023 at Shahid Chamran University of Ahvaz and Shiraz University. Twelve rats were divided into two groups (n = 6). Rats of the first group (control) were injected with artificial cerebrospinal fluid with a volume of 5 microliters ICV. Rats of the second group injected ICV with sCT at a dose of 1.5 nmol with a volume of 5 microliters. 72 hours after ICV injections, the serum levels of LH, FSH, and testosterone were measured. Also, sperms from the epididymal region were used to determine sperm quality.
Findings: ICV injection of sCT decreased the levels of LH, FSH, and testosterone significantly. The sCT reduced sperm density significantly, but not motility, compared to the control group. The sCT decreased significantly the intact acrosome and increased the damaged acrosome compared to the control group.
Conclusion: ICV injection of sCT can reduce sperm quality, probably through induction of an imbalance in FSH and LH production as well as testosterone.

Keywords

Main Subjects

References

  1. Son YL, Ubuka T, Tsutsui K. Regulation of stress response on the hypothalamic-pituitary-gonadal axis via gonadotropin-inhibitory hormone. Front Neuroendocrinol 2022; 64: 100953.
  2. George AJ, Dong B, Lail H, Gomez M, Hoffiz YC, Ware CB, et al. The E3 ubiquitin ligase RNF216/ TRIAD3 is a key coordinator of the hypothalamic-pituitary-gonadal axis. iScience 2022; 25(6): 104386.
  3. Zirkin BR, Papadopoulos V. Leydig cells: formation, function, and regulation. Biol Reprod 2018; 99(1): 101-11.
  4. Jiang T, Osadchiy V, Santamaria A, Zheng MH, Modiri N, Sigalos JT, et al. Initial gonadotropin levels and sperm parameters differentiate the response to clomiphene citrate in subfertile men. Transl Androl Urol 2022; 11(2): 116-23.
  5. Rahimova A, Zaidi M, Huang CL. Biology of calcitonin as an osteoprotective agent. 2020; 599-607.
  6. Kamgar-Parsi K, Tolchard J, Habenstein B, Loquet A, Naito A, Ramamoorthy A. Structural biology of calcitonin: from aqueous therapeutic properties to amyloid aggregation. Isr J Chem 2017; 57(7-8): 634-50.
  7. Huang C, Sun L, Moonga B, Zaidi M. Molecular physiology and pharmacology of calcitonin. Cell Mol Biol (Noisy-le-grand) 2006; 52(3): 33-43.
  8. Chesnut 3rd CH, Silverman S, Andriano K, Genant H, Gimona A, Harris S, et al. A randomized trial of nasal spray salmon calcitonin in postmenopausal women with established osteoporosis: the prevent recurrence of osteoporotic fractures study. Am J Med 2000; 109(4): 267-76.
  9. Chesnut 3rd, Azria M, Silverman S, Engelhardt M, Olson M, Mindeholm L. Salmon calcitonin: a review of current and future therapeutic indications. Osteoporos Int 2008; 19(4): 479-91.
  10. Haddadi K, Asadian L, Isazade A. Effects of nasal calcitonin vs. oral gabapentin on pain and symptoms of lumbar spinal stenosis: a clinical trial study. Clin Med Insights Arthritis Musculoskelet Disord 2016; 9: 133-8.
  11. Karponis A, Rizou S, Pallis D, Zafeiris C, Georgiou D, Galanos A, et al. Analgesic effect of nasal salmon calcitonin during the early post-fracture period of the distal radius fracture. J Musculoskelet Neuronal Interact 2015; 15(2): 186-9.
  12. Henriksen K, Byrjalsen I, Andersen JR, Bihlet AR, Russo LA, Alexandersen P, et al. A randomized, double-blind, multicenter, placebo-controlled study to evaluate the efficacy and safety of oral salmon calcitonin in the treatment of osteoporosis in postmenopausal women taking calcium and vitamin D. Bone 2016; 91: 122-9.
  13. Karsdal MA, Byrjalsen I, Alexandersen P, Bihlet A, Andersen JR, Riis BJ, et al. Treatment of symptomatic knee osteoarthritis with oral salmon calcitonin: results from two phase 3 trials. Osteoarthritis Cartilage 2015; 23(4): 532-43.
  14. Knopp-Sihota JA, Newburn-Cook CV, Homik J, Cummings GG, Voaklander D. Calcitonin for treating acute and chronic pain of recent and remote osteoporotic vertebral compression fractures: a systematic review and meta-analysis. Osteoporos Int
    2012; 23(1): 17-38.
  15. Omirinde JO, Azeez IA. Neuropeptide Profiles of Mammalian Male Genital Tract: Distribution and Functional Relevance in Reproduction. Front Vet Sci 2022; 9: 842515.
  16. Zhu B, Liu Q, Lin LI, Zheng X. Reductions in calcitonin gene-related peptide may be associated with the impairment of the contralateral testis in unilateral cryptorchidism. Exp Ther Med 2015; 9(5): 1797-800.
  17. Rahimi K, Sajedianfard J, Owji AA. Effects of salmon calcitonin on the concentrations of monoamines in periaqueductal gray in formalin test. Balkan Med J 2019; 36(5): 263-9.
  18. Adamkovicova M, Toman R, Martiniakova M, Omelka R, Babosova R, Krajcovicova V, et al. Sperm motility and morphology changes in rats exposed to cadmium and diazinon. Reprod Biol Endocrinol 2016; 14(1): 42.
  19. Cooper CW, Peng TC, Obie JF, Garner SC. Calcitonin-like immunoreactivity in rat and human pituitary glands: histochemical, in vitro, and in vivo studies. Endocrinology 1980; 107(1): 98-107.
  20. Flynn JJ, Margules DL, Cooper CW. Presence of immunoreactive calcitonin in the hypothalamus and pituitary lobes of rats. Brain Res Bull 1981; 6(4-6): 547-59.
  21. Gamakharia S, Le Foll C, Rist W, Baader-Pagler T, Baljuls A, Lutz TA. The calcitonin receptor is the main mediator of LAAMA's body weight lowering effects in male mice. Eur J Pharmacol 2021; 908: 174352.
  22. Wang PS, Tsai SC, Hwang GS, Wang SW, Lu CC, Chen JJ, et al. Calcitonin inhibits testosterone and luteinizing hormone secretion through a mechanism involving an increase in camp production in rats. J Bone Miner Res 1994; 9(10): 1583-90.
  23. Aaron L, Franco OE, Hayward SW. Review of prostate anatomy and embryology and the etiology of benign prostatic hyperplasia. Urol Clin North Am 2016; 43(3): 279-88.
  24. Foran D, Chen R, Jayasena CN, Minhas S, Tharakan T. The use of hormone stimulation in male infertility. Curr Opin Pharmacol 2023; 68: 102-33.
  25. Panza S, Giordano F, De Rose D, Panno ML, De Amicis F, Santoro M, et al. FSH-R Human Early Male Genital Tract, Testicular Tumors and Sperm: Its Involvement in Testicular Disorders. Life (Basel) 2020; 10(12): 336-41.
  26. Mungan NA, Mungan G, Basar MM, Baykam M, Atan A. Effect of seminal plasma calcitonin levels on sperm mobility. Arch Androl 2001; 47(2): 113-7.
  27. Drake MT, Clarke BL, Khosla S. Bisphosphonates: mechanism of action and role in clinical practice. Mayo Clin Proc 2008; 83(9): 1032-45.
  28. Ishii J, Katayama S, Itabashi A, Takahama M, Kawazu S. Salmon calcitonin induces pituitary tumor in rats. Endocrinol Jpn 1991; 38(6): 705-9.
  29. Cosman F, de Beur SJ, LeBoff MS, Lewiecki EM, Tanner B, Randall S, et al. Clinician's guide to prevention and treatment of osteoporosis. Osteoporos Int 2014; 25(10): 2359-81.
  30. Sengupta P, Dutta S, Karkada IR, Chinni SV. Endocrinopathies and Male Infertility. Life (Basel) 2022; 12(1): 10.
Journal of Isfahan Medical School
Volume 41, Issue 725
1st Week, September
September and October 2023
Pages 511-516

Files

History

  • Receive Date: 20 June 2023
  • Revise Date: 22 July 2023
  • Accept Date: 22 July 2023

Share

How to cite

Statistics