The Effect of High-Intensity Intermittent Exercise on Muscle Damage in Male Wistar Rat

Document Type : Original Article (s)

Authors

1 MSc, Department of Sport Physiology, Ilam Branch, Islamic Azad University, Ilam, Iran

2 Assistant Professor, Department of Sport Physiology, Ilam Branch. Islamic Azad University, Ilam, Iran

3 Associate Professor, Department of Pathology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran

Abstract

Background: High-intensity intermittent exercise (HIIE) is an increasingly popular exercise program that provides positive results with short sessions. The aim of this study was to evaluate the effect of one session of HIIE on muscle damage and inflammation.
Methods: Twenty-five male Wistar rats were randomly divided into three groups: control (Control, n = 8), HIIE, which were euthanized one hour after training (HIIE-1h, n = 8), and 24 hours after training (HIIE-24h, n = 9). HIIE group was subjected to a high-intensity interval exercise (VO2max 85-100%) that included ten exercise-recovery sets which was composed of 10 bouts of high intensity exercise of 1 min each (85-100% of VO2max), at 28 m/min, 10° inclinations on the treadmill interspersed by 2 min of active recovery, at 10 m/min, with no inclination. Serum IL-6 and IL-8 levels and the skeletal muscle inflammation and damage were assessed. Analysis of variance at 95% confidence level was used to analyze the data.
Findings: Serum IL-6 and IL-8 levels in the HIIE-1h and HIIE-24h groups showed a significant increase compared to the control group. Also, the pathology results showed that the inflammation and rhabdomyolysis of muscle fibers in the tibialis anterior muscle in the HIIE-24h and HIIE-1h groups were significant compared to the control group.
Conclusion: An HIIE session leads to inflammation and rhabdomyolysis in skeletal muscle. Serum IL-6 and
IL-8 levels also showed a significant increase that could be associated with muscle damage and inflammation.

Keywords

References

  1. Li J, Li Y, Atakan MM, Kuang J, Hu Y, Bishop DJ, et al. The molecular adaptive responses of skeletal muscle to high-intensity exercise/training and hypoxia. Antioxidants (Basel) 2020; 9(8): 656.
  2. Kyriakidou Y, Wood C, Ferrier C, Dolci A, Elliott B. The effect of Omega-3 polyunsaturated fatty acid supplementation on exercise-induced muscle damage. J Int Soc Sports Nutr 2021; 18(1): 9.
  3. Owens DL, Twist C, Cobley JN, Howatson G, Close GL. Exercise-induced muscle damage: What is it, what causes it and what are the nutritional solutions? Eur J Sport Sci 2019; 19(1): 71-85.
  4. Ives JS, Bloom S, Matias A, Morrow N, Martins N, Roh Y, et al. Effects of a combined protein and antioxidant supplement on recovery of muscle function and soreness following eccentric exercise. J Int Soc Sports Nutr 2017; 14: 21.
  5. Wan JJ, Qin Z, Wang PY, Sun Y, Liu X. Muscle fatigue: General understanding and treatment. Exp Mol Med 2017; 49: e384.
  6. Peak JM, Neubauer O, Della Gatta PA, Nosaka K. Muscle damage and inflammation during recovery from exercise. J Appl Physiol 2017; 122(3): 559-70.
  7. Wiewelhove T, Fernandez-Fernandez J, Raeder C, Kappenstein J, Meyer T, Kellmann M, et al. Acute responses and muscle damage in different high-intensity interval running protocols. J Sports Med Phys Fitness 2015.
  8. Pedersen BK. Anti-inflammatory effects of exercise. Role in diabetes and cardiovascular disease. Eur J Clin Invest 2017; 47(8): 600-11.
  9. Paulsen G, Mikkelsen UR, Raastad T, Peake JM. Leucocytes, cytokines and satellite cells: what role do they play in muscle damage and regeneration following eccentric exercise? Exerc Immunol Rev 2012; 18: 42-97.
  10. Freitas DA, Rocha-Vieira E, Soares BA, Nonato LF, Fonseca SR, Martins JB, et al. High intensity interval training modulates hippocampal oxidative stress, BDNF and inflammatory mediators in rats. Physiol Behav 2018; 184: 6-11.
  11. Idorn M, Thor Straten P. Exercise and cancer: from “healthy” to “therapeutic”? Cancer Immunol Immunother 2017; 66(5): 667-71.
  12. Gleeson M, Bishop NC, Stensel DJ, Lindley MR, Mastana SS, Nimmo MA. The anti-inflammatory effects of exercise: mechanisms and implications for the prevention and treatment of disease. Nat Rev Immunol 2011; 11(9): 607-15.
  13. Maggio M, Guralnik JM, Longo DL, Ferrucci L. Interleukin-6 in aging and chronic disease: a magnificent pathway. J Gerontol A Biol Sci Med Sci 2006; 61(6): 575-84.
  14. Pedersen BK, Febbraio MA. Muscle as an endocrine organ: focus on muscle-derived interleukin-6. Physiol Rev 2008; 88(4): 1379-406.
  15. Ostrowski K, Rohde T, Asp S, Schjerling P, Pedersen BK. Chemokines are elevated in plasma after strenuous exercise in humans. Eur J Appl Physiol 2001; 84(3): 244-5.
  16. Suzuki KA, Nakaji SH, Yamada M, Liu Q, Kurakake S, Okamura N, et al. Impact of a competitive marathon race on systemic cytokine and neutrophil responses. Med Sci Sports Exerc 2003; 35(2): 348-55.
  17. Chan MS, Carey AL, Watt MJ, Febbraio MA. Cytokine gene expression in human skeletal muscle during concentric contraction: evidence that IL-8, like IL-6, is influenced by glycogen availability. Am J Physiol Regul Integr Comp Physiol 2004; 287(2): R322-7.
  18. Akerstrom T, Steensberg A, Keller P, Keller C, Penkowa M, Pedersen BK. Retraction: Exercise induces interleukin-8 expression in human skeletal muscle. J Physiol 2011; 589(Pt 13): 3407.
  19. Croft L, Bartlett JD, MacLaren DPM, Reilly T, Evans L, Mattey DL, et al. High-intensity interval training attenuates the exercise-induced increase in plasma IL-6 in response to acute exercise. Appl Physiol Nutr Metab 2009; 34(6): 1098-107.
  20. Gómez AM, Martinez C, Fiuza-Luces C, Herrero F, Pérez M, Madero L, et al. Exercise training and cytokines in breast cancer survivors. Int J Sports Med 2011; 32(06): 461-7.
  21. Clarkson PM, Kearns AK, Rouzier P, Rubin R, Thompson PD. Serum creatine kinase levels and renal function measures in exertional muscle damage. Med Sci Sports Exerc 2006; 38: 623-7.
  22. Baltopoulos P. Exercise induced modulation of immune system functional capacity. Biol Exerc 2009; 5(1): 39-49.
  23. Shankar G, Sinha AG, Sandhu JS. Pulsed ultrasound does not affect recovery from delayed onset muscle soreness. Online J Health Allied Scs 2006; 5(1): 1-6.
  24. Deminice R, Rosa FT, Franco GS, Jordao AA, de Freitas EC. Effects of creatine supplementation on oxidative stress and inflammatory markers after repeated-sprint exercise in humans. Nutrition 2013; 29(9): 1127-32.
  25. Nielsen AR, Pedersen BK. The biological roles of exercise-induced cytokines: IL-6, IL-8, and IL-15. Appl Physiol Nutr Metab 2007; 32(5): 833-9.
  26. Pinto AP, da Rocha AL, Kohama EB, Gaspar RC, Simabuco FM, Frantz FG, et al. Exhaustive acute exercise-induced ER stress is attenuated in IL-6-knockout mice. J Endocrinol 2019; 240(2): 181-93.
  27. Paulsen G, Egner IM, Drange M, Langberg H, Benestad HB, Fjeld JG, et al. A COX-2 inhibitor reduces muscle soreness, but does not influence recovery and adaptation after eccentric exercise. Scand J Med Sci Sports 2010; 20(1): e195-207.
  28. Raastad T, Owe SG, Paulsen G, Enns D, Overgaard K, Crameri R, et al. Changes in calpain activity, muscle structure, and function after eccentric exercise. Med Sci Sports Exerc 2010; 42(1): 86-95.
Journal of Isfahan Medical School
Volume 40, Issue 682
2nd Week, October
September and October 2022
Pages 593-601

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History

  • Receive Date: 15 August 2022
  • Accept Date: 02 October 2022

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