اثر تمرینات مقاومتی به همراه مصرف عصاره‌ی زعفران بر بیان ژن‌های Bax، Bcl-2 و Caspase-3 در بافت هیپوکامپ رت‌های نر آلزایمری القا شده

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکترای تخصصی فیزیولوژی ورزشی، دانشکده‌ی علوم انسانی، گروه تربیت بدنی و علوم ورزشی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران

2 استاد فیزیولوژی ورزشی، دانشکده‌ی علوم انسانی، گروه تربیت بدنی و علوم ورزشی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران

3 دانشیار، دانشکده‌ی علوم انسانی، گروه تربیت بدنی و علوم ورزشی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران

چکیده

مقاله پژوهشی




مقدمه: آلزایمر، یک بیماری مخرب با اختلالات پیشرونده است که با کاهش حافظه همراه و عدم وجود درمان قطعی برای آن می‌باشد، هدف از پژوهش حاضر، بررسی اثر تمرینات مقاومتی به همراه مصرف عصاره‌ی زعفران بر بیان ژن‌های Bax، Bcl-2 و Caspase-3 در بافت هیپوکامپ رت‌های نر آلزایمری القا شده بود.
روش‌ها: در مطالعه‌ی تجربی حاضر، 32 سر رت نر بالغ به صورت تصادفی به 4 گروه آلزایمری شامل گروه‌های شاهد، تمرینات هوازی، تمرینات هوازی + عصاره‌ی زعفران و عصاره‌ی زعفران تقسیم شدند. آلزایمر با تزریق آمیلوئید بتا 42-1 در هیپوکامپ ایجاد شد. تمرینات مقاومتی شامل 3 جلسه در هفته به مدت 8 هفته انجام گردید. برای تعیین میزان بیان ژن‌های مورد نظر از روش Real Time PCR و جهت تجزیه و تحلیل داده‌ها از آزمون آنالیز واریانس یک طرفه و از آزمون تعقیبی Tom Hen استفاده شد.
یافته‌ها: نتایج نشان داد، بعد از 8 هفته تمرین مقاومتی، بیان ژن Bax در گروه‌های مداخله نسبت به گروه شاهد کاهش معنی‌دار یافت. همچنین بیان ژن Bcl-2 در گروه شاهد و تمرینات مقاومتی و گروه تمرینات مقاومتی و عصاره نسبت به گروه شاهد کاهش معنی‌داری را نشان داد. بیان ژن caspase3 در گروه شاهد + تمرینات مقاومتی و گروه تمرینات مقاومتی و عصاره نسبت به گروه شاهد، کاهش معنی‌داری را نشان داد.
نتیجه‌گیری: به نظر می‌رسد تمرین مقاومتی و مصرف عصاره‌ی زعفران موجب کاهش بیان ژن‌های دخیل در آپوپتوز رت‌های نر مبتلا به آلزایمر می‌گردد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Resistance Training Along with the Consumption of Saffron Extract on the Expression of Bax, Bcl-2 and Caspase-3 Genes in the Hippocampal Tissue of Male Alzheimer's Induced Rats

نویسندگان [English]

  • Fazel Bazyar 1
  • Ramin Shabani 2
  • َAlireza Elmiyeh 3
1 PhD Candidate, School of Human Sciences, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
2 Professor of Sports Physiology, School of Humanities, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
3 Associate Professor, School of Humanities, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
چکیده [English]

Background: Considering that Alzheimer's is a destructive disease with progressive disorders and memory loss and there is no definitive treatment for it, the purpose of this research is to investigate the effect of resistance training along with the consumption of saffron extract on the expression of Bax, Bcl-2 and Caspase genes. 3-Alzheimer was induced in the hippocampal tissue of male Alzheimer's rats.
Methods: In this experimental study, 32 adult rats were randomly divided into 4 Alzheimer's groups, including control groups, aerobic exercises, aerobic exercises + saffron extract, and saffron extract. Alzheimer's was induced by injecting amyloid beta 42-1 into the hippocampus. Resistance exercises including 3 sessions per week were performed for 8 weeks. Real Time PCR method was used to determine the expression level of the target genes. The data were analyzed using one-way analysis of variance and Tom Hen's post hoc test.
Findings: The results showed that after 8 weeks of resistance training, the expression of Bax gene in the intervention groups significantly decreased compared to the control group. Also, the expression of Bcl-2 gene in the control and resistance training group and the resistance training and extract group showed a significant decrease compared to the control group. The expression of caspase3 gene in the control + resistance training group and the resistance training and extract group showed a significant decrease compared to the control group.
Conclusion: It seems that resistance training and consumption of saffron extract decrease the expression of genes involved in apoptosis in Alzheimer's male rats.

کلیدواژه‌ها [English]

  • Resistance training
  • Saffron
  • Bcl-2
  • Caspase-3
  • Alzheimer's

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مجله دانشکده پزشکی اصفهان
دوره 41، شماره 713
هفته 1 خرداد
خرداد و تیر 1402
صفحه 206-214

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  • تاریخ دریافت: 18 بهمن 1401
  • تاریخ بازنگری: 12 اردیبهشت 1402
  • تاریخ پذیرش: 16 اردیبهشت 1402

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