بررسی اثر تجویز مادری کندر در دوران شیردهی بر شاخص‌های استریولوژیک هیپوکامپوس موالید موش بزرگ آزمایشگاهی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشیار، گروه بیولوژی و علوم تشریح، دانشکدهی پزشکی، دانشگاه علوم پزشکی شهید صدوقی یزد، یزد، ایران

2 استاد، گروه علوم تشریحی و بیولوژی مولکولی، دانشکدهی پزشکی دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

مقدمه: پیش از این گزارش کردیم که تجویز کندر در دوره‌ی شیردهی مادر باعث افزایش یادگیری و حافظه‌ی زاده‌های رت جوان شد. این مطالعه با هدف تعیین تغییرات احتمالی ساختمان هیپوکامپوس به عنوان یک مرکز حیاتی دخیل در روند یادگیری و حافظه متعاقب تجویز کندر در دوره‌ی شیردهی انجام شد.روش‌ها: در این مطالعه‌ی تجربی، رت‌های نر دو ماهه‌ی ویستار که به مادران آن‌ها در دوره‌ی شیردهی به مدت سه هفته به صورت روزانه 100 میلی‌گرم به ازای هر کیلوگرم وزن بدن عصاره‌ی آبی کندر خورانده شده بود و گروه شاهد که آب مقطر دریافت کرده بودند (در هر گروه 6 سر رت) به طور عمیق بیهوش شدند. مغز هر یک از رت‌ها بیرون آورده شد و به دو نیمکره تقسیم شد. یک نیمکره برای محاسبه‌ی حجم لایه‌های سلولی و تعداد کل نورون زیر نواحی و دیگری برای تخمین حجم نورون‌های هیپوکامپوس استفاده شد. به ترتیب روش‌های استریولوژیک کاوالیه، Optical fractionator و روتاتور برای تعیین حجم لایه‌‌ها، تعداد نورون‌ها و حجم جسم سلولی نورون‌های مربوطه استفاده شد.یافته‌ها: تعداد کل نورون‌ها در زیر نواحی هیپوکامپوس گروه آزمایش در مقایسه با گروه شاهد تفاوت معنی‌داری نداشت، اما حجم لایه‌های سلولی شکنج دندانه‌ای و شاخ آمون 3 و حجم نورون‌های این زیر نواحی متعاقب مصرف کندر توسط مادر در دوره‌ی شیردهی افزایش یافت.نتیجه‌گیری: افزایش حجم جسم سلولی نورون‌های هیپوکامپوس زاده‌های رت متعاقب مصرف کندر در دوره‌ی شیردهی توسط مادر را می‌توان به عنوان شاخصی برای بهبود عملکرد هیپوکامپوس(حافظه) در نظر گرفت که ممکن است به علت افزایش محتویات نورون از قبیل میانجی‌های عصبی و ارگانل‌های داخل سلولی یا افزایش تعداد تماس‌های سیناپسی باشد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effects of Maternal Administration of Boswellia Gum Resin (Frankin-cense) during Lactation on Stereological Parameters of Rat Hippocampus

نویسندگان [English]

  • Mohammad Hosseini Sharifabad 1
  • Ebrahim Esfandiari 2
1 Associate Professor, Department of Cell Biology and Anatomy, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2 Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده [English]

Background: We have previously reported that maternal administration of Boswellia serrata gum resin (Frankincense) during lactation increased learning and memory performance in young rat offsprings. This study aimed to determine the likely structural alterations of hippocampus, as a vital centre involved in learning and memory, following administration of Frankincense during lactationMethods: In this experimental study, 2 month-old male Wistar rats whose mothers were given oral aqueous extract of the Boswellia serrata (100 mg/kg/day) during lactation (3 weeks), and their controls whose mothers received saline (n = 6) were anesthetized and transcardially perfused. All brains were removed and divided into two hemispheres. One hemisphere was selected for estimating the volumes of cellular layers of hippocampus and the total number of neurons, and the other for estimating the individual somal volume. The Cavalieri's principle and an optical fractionator and a rotator were employed to estimate the volumes of layers, total number of neurons and individual neuronal volume, respectively.Findings: Our results showed that no significant difference in the total number of neurons in hippocampal subregions of the experimental group compared to the controls. However, the volume of cellular layer of dentate gyrus and cornu ammon 3(CA3) and individual volume of their neurons increased following maternal administration of Frankincense during lactation. Conclusion: The increased perikaryal volume in the hippocampal neurons of offsprings following maternal administration of Frankincense during lactation might be considered as an indicator of improved function of hippocampus, i.e. memory. It might be due to the increases in contents of neurons such as neurotransmitters, intracellular organelles or the number of synaptic contacts.

کلیدواژه‌ها [English]

  • Development
  • Frankincense
  • Hippocampus
  • Rat

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مجله دانشکده پزشکی اصفهان
دوره 29، شماره 165 - شماره پیاپی 165
بهمن و اسفند 1390
صفحه 2198-2207

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  • تاریخ دریافت: 25 تیر 1390
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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