بررسی بیان سیکلواکسیژناز 2 در سرطان سلول‌های سنگفرشی سر و گردن

مختاری, مژگان; هدایت, پگاه

بررسی بیان سیکلواکسیژناز 2 در سرطان سلول‌های سنگفرشی سر و گردن

نوع مقاله : مقاله های پژوهشی

نویسندگان

¹ استاد، گروه پاتولوژی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

² دانشجوی پزشکی، کمیته‌ی تحقیقات دانشجویی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

مقدمه: بیان سیکلواکسیژناز 2، در بافت‌های سرطانی با پیش‌آگهی بدتر در ارتباط است. هدف از انجام مطالعه‌ی حاضر، تعیین فراوانی بیان سیکلواکسیژناز 2 در بیماران مبتلا به سرطان سلول‌های سنگفرشی سر و گردن بود.روش‌ها: طی یک مطالعه‌ی توصیفی- تحلیلی که بین سال‌های 94-1390 در دو بیمارستان الزهرا (س) و آیت‌اله کاشانی اصفهان صورت گرفت، با استفاده از تکنیک Real-time polymerase chain reaction (Real-time PCR) میزان بیان سیکلواکسیژناز 2 در نمونه‌های بافتی خارج شده توسط جراحی بررسی و ارتباط بیان سیکلواکسیژناز 2 با داده‌های بالینی و پیش‌آگهی دهنده ارزیابی گردید.یافته‌ها: 30 نمونه از سرطان سلول‌های سنگفرشی سر و گردن مورد مطالعه قرار گرفت که تنها 4 مورد (3/13 درصد) سیکلواکسیژناز 2 مثبت بودند. دو گروه سیکلواکسیژناز 2 مثبت و منفی از نظر توزیع سنی جنسی، اندازه‌ی تومور، درگیری لنف نود، متاستاز و بافت‌شناسی تومور اختلاف معنی‌داری نداشتند، اما عود بیماری در گروه بیان کننده‌ی سیکلواکسیژناز 2 به طور معنی‌داری بیشتر بود (005/0 = P).نتیجه‌گیری: سرطان سلول‌های سنگفرشی سر و گردن با بیان سیکلواکسیژناز 2 دارای پیش‌آگهی و خصوصیات بالینی بافت‌شناسی بدتری هستند و این نشانگر، می‌تواند به عنوان یکی از نشانگرهای پیش‌آگهی دهنده در سرطان سلول‌های سنگفرشی سر و گردن و نیز به عنوان هدف درمانی در نظر گرفته شود. همچنین، مطالعات بیشتری در این زمینه پیشنهاد می‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

Cyclooxygenase-2 Expression in Squamous Cell Carcinoma of the Head and Neck

نویسندگان [English]

Mozhgan Mokhtari ¹
Pegah Hedayat ²

¹ Professor, Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

² Student of Medicine, Student Research Committee, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

چکیده [English]

Background: Cyclooxygenases-2(COX) expression in cancer tissues is associated with worse prognosis of cancers. We studied cyclooxigenase-2 expression among patients with head and neck squamous cell carcinoma (HNSCC).Methods: In a cross-sectional study performed in two university hospitals in Isfahan City, Iran, between the years 2011 and 2015, patients with newly pathological diagnosis of HNSCC were evaluated. Real-time polymerase chain reaction (real-time PCR) was used to evaluate the expression of cyclooxigenase-2 in surgical excised cancer tissues. Patients were followed-up for one year regarding recurrence and mortality. Association of cyclooxigenase-2 expression with clinicopathologic data and prognosis were analyzed.Findings: Thirty cases of HNSCC with mean age of 59.6 years (70% men) were studied. Only 4 cases (13.3%) were cyclooxigenase-2 positive. Compared with cyclooxigenase-2 negative cases, those with cyclooxigenase-2 expression were relatively, but not significantly, older (71.0 ± 12.5 vs. 57.8±12.0 years, P = 0.054) and had larger tumor size (2.70 ± 1.19 vs. 1.76 ± 0.84 cm, P = 0.061), more frequent moderately/poorly differentiated tumors (50.0 vs. 15.3 percent, P = 0.190), and more frequent involvement of the lymph nodes (50.0 vs. 7.6 percent, P = 0.075). One of the cyclooxigenase-2 positive cases had metastasis. After one year follow-up, recurrence rate was 75.0% and 9.5% in cyclooxigenase-2 positive and negative cases, respectively (P = 0.005).Conclusion: We found that cyclooxigenase-2 expression in HNSCC patients is associated with worse clinicopathologic characteristics and prognosis. It may be used as a prognostic factor for HNSCC and also a target for treatment. Further studies with larger sample size are warranted in this regard.

کلیدواژه‌ها [English]

Biological Markers
Carcinoma
Squamous cell
Head and neck neoplasms
Cyclooxygenase

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