Mechanical Behavior Obtaining of Cardiac Contracting Cell, Using Atomic Force Microscopy (AFM) and Analytical Relations

Document Type : Original Article (s)

Authors

1 Department of Biomechanics, School of Mechanical Engineering, Iran University of Science and Technology, Tehran, Iran

2 Associate Professor, Department of Biomechanics, School of Mechanical Engineering, Iran University of Science and Technology, Tehran, Iran

3 Associate Professor, Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Abstract

Background: Cardiac contracting cells cause heart pumping motion by its contraction and any problem in this motion is related to these cells. In one approach, considering cardiac cell as a homogenous body with linear elastic properties, it can be analytically mechanically modeled. To obtain cell elastic modulus by existing analytical relations, experimental data are needed.Methods: In this study, suitable experimental data were collected from mouse cardiac cells using atomic force microscopy (AFM). Then, by contact mechanics relations for this experimental method, elastic modulus were obtained and analyzed with two methods.Findings: Using two methods, elastic moduli were 48.08 ± 2.26 and 55.67 ± 2.56 kPa. Variations of elastic modulus by indentation depth showed nonlinear elastic properties of these cells.Conclusion: It can be concluded that via both of the presented methods, the same results for elastic modulus value for these contracting cells were obtained approximately. Also, it could be said that the linear Hertz’s model is suitable for initial prediction of the cell mechanical properties and to more exact and general evaluate of these properties, it is better to apply nonlinear models.

Keywords

References

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Journal of Isfahan Medical School
Volume 32, Issue 290 - Serial Number 290
May and June 2014
Pages 947-956

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History

  • Receive Date: 05 September 2013
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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