اثر یک دوره بازتمرینی بر بیان ژن‌های AKT, PDK1, PIP3 عضله‌ی کف پایی موش‌های صحرایی نر

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 کارشناس ارشد فیزیولوژی ورزشی، گروه علوم ورزشی، دانشکده‌ی ادبیات و علوم انسانی، دانشگاه ولی‌عصر رفسنجان، رفسنجان، ایران

2 دانشیار، گروه علوم ورزشی، دانشکده‌ی ادبیات و علوم انسانی، دانشگاه ولی‌عصر رفسنجان، رفسنجان، ایران

چکیده

مقاله پژوهشی




مقدمه: بی‌تحرکی طولانی‌مدت، ساختار و عملکرد عضلات اسکلتی را به طور قابل توجهی تحت تأثیر قرار می‌‌دهد. پژوهش حاضر با هدف بررسی تأثیر برنامه‌ی باز‌تمرینی با تمرین مقاومتی بر تغییرات ژن‌های AKT, PDK1, PIP3 درگیر در مسیر سنتز و تجزیه‌ی پروتئین پس از یک دوره‌ی بی‌تحرکی در عضله‌ی کف پایی (پلانتاریس) موش‌های صحرایی نر انجام شد.
روش‌ها: در این مطالعه‌ی تجربی 30 سر موش صحرایی نر از نژاد ویستار به چهار گروه معلق، باز‌تمرین، بی‌تمرین و گروه تمرینی به صورت تصادفی تقسیم شدند. مدت برنامه‌ی تمرینی 4 هفته و هر هفته 3 جلسه بود. حیوانات باید از یک نردبان عمودی با وزنه‌هایی که به دم آن‌ها اضافه شده بود، بالا می‌رفتند. موش‌ها پس از 48 ساعت از آخرین جلسه‌ی تمرین، بی‌هوش و عضله کف پایی استخراج گردید. به منظور اندازه‌گیری بیان ژن‌های موردنظر از روش Real-Time PCR استفاده شد. تجزیه و تحلیل داده‌ها و تفاوت‌های بین گروهی، با استفاده از آزمون‌های پارامتریک Levene، Independent Sample T-test، One-way ANOVA و آزمون تعقیبی Tukey در سطح معنی‌داری، 0/05 ˂ P تجزیه و تحلیل شدند.
یافته‌ها: بیان ژن‌های AKT, PDK1, PIP3 در عضله‌ی کف پایی موش‌های صحرایی نر در گروه بازتمرینی نسبت به سایر گروه‌ها تفاوت معنی‌داری دارد (به ترتیب 0/024 = P، 0/0001 = P  و 0/0001 = P).
نتیجه‌گیری: به نظر می‌رسد که تمرین مقاومتی قبل از تعلیق اندام تحتانی و همچنین بعد از بی‌تحرکی، سبب کاهش آتروفی در عضله‌ی کف پایی موش‌های صحرایی نر می‌شود. از طرفی دیگر تعلیق اندام تحتانی بیان ژن‌های آترفیک (AKT, PDK1, PIP3) در عضله‌ی کف پایی موش‌ها را افزایش می‌دهد.

تازه های تحقیق

عبدالرضا کاظمی: Google Scholar

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of a Retraining Course on the Expression of AKT, PDK1, and PIP3 Genes in the Plantaris Muscle of Male Rats

نویسندگان [English]

  • Vahid Ghanbari Mazidi 1
  • Abdolreza Kazemi 2
1 MSc, Department of Sports Sciences, School of Literature and Human Sciences, Vali Asr University of Rafsanjan, Rafsanjan, Iran
2 Associate Professor, Department of Physical Education, School of Letters and Humanities, Vali E-Asr University, Rafsanjan, Iran
چکیده [English]

Background: Long-term inactivity significantly affects the structure and function of skeletal muscles. The purpose of this study was to investigate the effect of retraining programs with resistance training on the changes of AKT, PDK1, and PIP3 genes involved in protein synthesis and breakdown after a period of inactivity in the plantaris muscle of male rats.
Methods: In this experimental study, 30 male Wistar rats were randomly divided into four suspension, retraining, non-training, and training groups. The training program was four weeks and three sessions per week. The animals had to climb a vertical ladder with weights attached to their tails. After 48 hours from the last training session, the mice were anesthetized, and the soleus muscle was extracted.  The expression of the desired genes was measured using the Real-Time PCR method. Data analysis and differences between groups were analyzed using Lone's parametric tests, independent t, one-way ANOVA, and Tukey's post hoc test at a significant level P ˂ 0.05.
Findings: The expression of AKT, PDK1, and PIP3 genes in the plantaris muscle of male rats in the retraining group has a significant difference compared to other groups (P = 0.027, P = 0.0001, and P = 0.0001, respectively).
Conclusion: Resistance training before lower limb suspension and after immobilization seems to reduce atrophy in plantaris muscle in male rats. On the other hand, lower limb suspension increases the expression of atrophic genes (AKT, PDK1, PIP3) in the plantaris muscle of rats.

کلیدواژه‌ها [English]

  • Resistance training
  • Muscle atrophy
  • Gene expression

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مجله دانشکده پزشکی اصفهان
دوره 42، شماره 769
هفته 1، مرداد
مرداد و شهریور 1403
صفحه 430-438

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  • تاریخ دریافت: 31 خرداد 1403
  • تاریخ بازنگری: 16 مرداد 1403
  • تاریخ پذیرش: 27 مرداد 1403

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