Identification New Angiotansin-1 Converting Enzyme Inhibitory Peptide from Ostrich Egg White Protein Hydrolysate

Document Type : Original Article (s)

Authors

1 PhD Student, Department of Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran

2 Associate Professor, Department of Chemistry, School of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

3 Professor, Department of Human Genetics, Immunology Research Center, Avicenna Research Institute AND Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

4 Assistant Professor, Department of Biology, School of Sciences, Islamic Azad University, Mashhad Branch, Mashhad, Iran

Abstract

Background: Due to the side effects of anti-hypertension drugs, extracting bioactive peptides from natural sources is of great importance. The mail goal of this study was identifying a peptide for enzyme hydrolytic of ostrich egg white protein hydrolysate (OEWPH) and investigating its inhibitory effects on angiotansin-1 converting enzyme (ACE).Methods: The ostrich egg white protein hydrolysate was prepared using pepsin and pancreatin and then fractionated using evaluated reveres-phase high-performance liquid chromatography (RP-HPLC). Tandem mass analysis of the purified peptide was used to reveal peptide sequence. ACE inhibitory effect and kinetic parameters of the reaction in the presence of the peptide was evaluated. Molecular docking was used to determine the interaction parameters of ACE-peptide comp.Findings: Peptide sequencing of the selected peptide revealed a DAESLSRLLG (MW = 1060/18 Da) and named DG-10. The DG-10 peptide showed a potent inhibitory effect on ACE with the half maximal inhibitory concentration (IC50) value of 0.133 mg/ml. Lineweaver-Burk plot exhibited a non-competitive behavior in the presence of the DG-10 peptide. Molecular interactions and energy binding of the peptide with ACE were investigated via molecular docking.Conclusion: The DG-10 peptide isolated from ostrich egg white protein hydrolysate displayed a potent inhibitory effect on ACE.

Keywords

References

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Journal of Isfahan Medical School
Volume 32, Issue 301 - Serial Number 301
July and August 2014
Pages 1496-1508

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History

  • Receive Date: 06 August 2014
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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