بررسی بیان ژن TOX3 در بیماران مبتلا به سرطان مری

احمدی بلوطکی, ثریا; عمادی بایگی, مجتبی; روحی, لیلا; شایسته, علی اکبر

بررسی بیان ژن TOX3 در بیماران مبتلا به سرطان مری

نوع مقاله : مقاله های پژوهشی

نویسندگان

¹ گروه ژنتیک، دانشکده‌ی علوم پایه، دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران

² استادیار، گروه ژنتیک، دانشکده‌ی علوم پایه و پژوهشکده‌ی بیوتکنولوژی، دانشگاه شهرکرد، شهرکرد، ایران

³ استادیار، گروه زیست‌شناسی تکوین جانوری، دانشکده‌ی علوم پایه، دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران

⁴ استادیار، گروه داخلی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی جندی‌شاپور اهواز، اهواز، ایران

چکیده

مقدمه: سرطان مری، ششمین سرطان منجر به مرگ در دنیا می‌باشد. اگر چه بروز این بیماری در سه دهه‌ی گذشته در ایران رو به کاهش بوده است، اما همچنان ششمین سرطان شایع در کشور می‌باشد. ژن TOX3 به خانواده‌ای بزرگ و متنوع از پروتئین‌های HMG-box تعلق دارد که روی کروموزوم 16q12 قرار گرفته است. مطالعه‌ی حاضر با هدف مقایسه‌ی بیان ژن TOX3 در بافت پارافینه‌ی سرطانی و غیر توموری مری انجام شد.روش‌ها: این مطالعه ، یک مطالعه‌ی مورد- شاهدی بود که بر روی تعداد 40 نمونه از بافت پارافینه‌ی سرطانی مری و بافت سالم آن انجام شد. نمونه‌ها از بیماران مراجعه کننده به بیمارستان امام خمینی (ره) اهواز در سال‌های 89-1384 جمع‌آوری گردید. RNA تام استخراج شد و پس از سنتز complementary DNA (cDNA)، سطوح بیان نسبی این ژن‌ها با استفاده از تکنیک Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) و به روش 2-ΔΔCT تعیین شد.یافته‌ها: میزان بیان ژن TOX3 در بافت‌های توموری مری نسبت به بافت‌های غیر توموری کاهش بیان داشت؛ هر چند که از نظر آماری، معنی‌‌دار نبود (10/0 < P).نتیجه‌گیری: در سرطان مری سنگفرشی، بیان ژن TOX3 در بافت‌های توموری مری نسبت به بافت‌های سالم کاهش می‌یابد. از این رو، با در نظر گرفتن کلیه‌ی نتایج بیان این ژن در منابع مختلف، می‌توان پیشنهاد نمود که ممکن است ژن TOX3 در سرطان مری به عنوان یک مهار کننده‌ی تومور عمل نماید. بنابراین، مطالعات بیشتر جهت تعیین دقیق مکانیسم عمل این ژن به عنوان یک مهار کننده‌ی تومور در SCC مورد نیاز است.

کلیدواژه‌ها

عنوان مقاله [English]

The Expression of TOX3 Gene in Patients with Esophageal Cancer

نویسندگان [English]

Soraya Ahmadi-Balootaki ¹
Modjtaba Emadi-Baygy ²
Leyla Rouhi ³
Ali Akbar Shayesteh ⁴

¹ Department of Genetics, School of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

² Assistant Professor, Department of Genetics, School of Basic Sciences AND Research Institute of Biotechnology, University of Shahrekord, Shahrekord, Iran

³ Assistant Professor, Department of Developmental Biology, School of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

⁴ Assistant Professor, Department of Internal Medicine, School of Medicine, Ahvaz Jondishapour University of Medical Sciences, Ahvaz, Iran

چکیده [English]

Background: Esophageal cancer is the sixth cause of cancer mortality worldwide. While it is the sixth prevalent cancer in Iran, its incidence has been decreasing during the past 3 decades. Belonging to the HMG-box protein family, TOX3 (TOX high-mobility group box family member 3) gene is located on 16q12 chromosome. This study aimed to compare the expression of TOX3 gene in cancerous and non-cancerous tissue of esophagus.Methods: In this case-control study, 40 formalin-fixed, paraffin-embedded (FFPE) samples of cases of esophageal Squamous cell carcinoma (SCC) and healthy tisuue were assessed. Samples were collected from patients referred to Imam Khomeini Hospital, Ahvaz, Iran, during 2005 to 210. Total RNA was extracted and complementary DNA (cDNA) was synthesied. The relative gene expression was determined using quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) and quantified using 2-ΔΔCT method.Findings: The expression of TOX3 gene was lower in tumor tissues compared to normal tissues. However, it was not statistically significant (P > 0.1).Conclusion: Our study revealed that the expression of TOX3 is lower in squamous esophageal cancer. Consequently, by considering all the data about the expression of TOX3 gene from various resources, it could be suggested that the TOX3 gene might act as a tumor suppressor in esophageal squamous cell carcinoma. Therefore, further studies should be done to elucidate the exact mechanism of action of the gene as a tumor suppressor in Squamous cell carcinoma.

کلیدواژه‌ها [English]

TOX3 gene
Real-time polymerase chain reaction
SYBR Green
Esophageal cancer

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