Investigation of the Effect of Adding Chitosan on the Skin Deposition of Activated Charcoal in Male Rat Model

Document Type : Original Article (s)

Authors

1 Associate Professor, Department of Histology, School of Medicine AND Maternal and Childhood Obesity Research Center, Urmia University of Medical Science, Urmia, Iran

2 General Practitioner, Department of Histology, School of Medicine, Urmia University of Medical Science, Urmia, Iran

3 Assistant Professor, Department of Pharmaceutics, School of Pharmacy AND Solid Tumor Research Center, Urmia University of Medical Science, Urmia, Iran

Abstract

Background: Activated carbon (charcoal) is a group of high-porosity carbonaceous materials that are highly regarded due to their high adsorption capacity, especially the absorption of toxins in the body. On the other hand, some studies have shown that the combination of activated charcoal with certain substances increases its adsorption cost. The aim of this study was to investigate the effect of adding chitosan on the skin deposition of activated charcoal in rats.Methods: In this experimental study, 15 adult male Wistar rats were divided into three groups of four and one group of three. In the first group, chitosan topical gel, in the second group, chitosan topical gel containing 5% activated charcoal, and in the third group, the gel containing activated charcoal was rubbed to the skin surface of the posterior part of the rats' trunk area measuring one cm2, for ten days. The fourth group did not receive any treatment during the study period. Finally, the treated area of skin was cut locally and was stained using hematoxylin-eosin, and the amount of activated charcoal uptake was determined and compared.Findings: The mean accumulation and penetration of activated charcoal was 23.0 ± 7.3 and 18.0 ± 4.9 in the groups of activated charcoal plus chitosan and activated charcoal only, respectively, and there was no statistically significant difference between the two groups. The highest accumulation and penetration of activated charcoal was observed in the group of activated charcoal plus chitosan.Conclusion: Activated charcoal in the presence of chitosan gel can pass through the horny and granulosa stratums of the rats’ skin. Therefore, in cases of need to more absorption, this combination can be useful.

Keywords

References

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Journal of Isfahan Medical School
Volume 37, Issue 540 - Serial Number 540
July and August 2019
Pages 1001-1006

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History

  • Receive Date: 08 July 2019
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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