A Survey on Association of P16 Protein and Uterine Cervical Dysplasia

Document Type : Original Article(s)

Authors

1 Assistant Professor of Pathology, Molecular Pathology Research Center, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran.

2 Molecular Pathology Research Center, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran.

3 Pathologist, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Abstract

Background: Cervical (uterine) carcinoma is the second most frequent type of women cancer. Success in early diagnosis of this disease is due to the use of pap-smear test. However, this test has both false-positive and false-negative results. P16 protein is a Cyclin-dependent Kinase inhibitor supposed to be overexpressed in cervical dysplasia and cancer. Our study was about correlation between P16 and cervical dysplasia.Methods: In this study 45 patients with dysplastic abnormalities in cervix (including Ascus-H) were sampled for papsmear (by Liquid-base method). Two smears were stained, one with papanicolau and the other (by IHC) for P16. The results were compared using t-test via SPSS15 software.Finding: Pap-stain revealed 13 ASCUS (29%), 18 LSIL (40%), 11 HSIL (24%), and 3 S.C.C (7%). Immunostaining of P16 protein was observed in ASCUS (3/13, 23%), LSIL (12/18, 67%), HSIL (11/11, 100%), and S.C.C (3/3, 100%). P16 had significant correlation with degree of cervical dysplasia in Liquid-base pap-smear (P < 0.0001).Conclusion: We suggest the usage of P16-test as an optional test for cervical dysplasia according to our study.

Keywords

References

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Journal of Isfahan Medical School
Volume 28, Issue 118 - Serial Number 118
November and December 2011
Pages 1207-1213

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History

  • Receive Date: 06 February 2011
  • Revise Date:
  • Accept Date: 06 April 2022

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