Cytotoxic Evaluation of Different Extracts of Taxus Baccata against MDA–MB–468, HeLa and K562 Cancer Cell Lines

Document Type : Original Article (s)

Authors

1 Professor, Department of Medicinal Chemistry, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

2 Pharm D Student, School of Pharmacy AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

3 Professor, Isfahan Pharmaceutical Sciences Center, Isfahan University of Medical Sciences, Isfahan, Iran

4 Department of Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Taxus baccata is known as a source of anticancer drug called taxol. This study was performed to confirm the consistency of results obtained by extraction of Taxanes by optimal solvent system using biological assessment.Methods: Three cell lines including MDA-MB-468, Hela and K562 were cultured in PRMI-1640 medium with 10% fetal bovine serum. Powder of aerial parts of Taxus baccata was extracted using 8 different solvent by maceration method. High performance thin layer chromatography (HPTLC) was used to standardize the extracts according to taxol content. Cytotoxicity of different concentrations of extracts (acetone 20, 50 and 100 %, ethanol 20, 50 and 100%, acetone dichloromethane (1-1), and pure methanol) was evaluated against cultured cells, using MTT assay.Findings: Acetone-dichloromrthane (1-1) then pure acetone extract were the most cytotoxic extracts. Meanwhile, between the three cell lines tested, the highest cytotoxic effect of the extracts was observed on K562 cells.Conclusion: The results of this study showed that among evaluated extracts, more cytotoxic effect wsa in acetone-dichloromethane (1-1) followed by pure acetone extract. Analysis of extracts showed that the highest amount of taxans has been extracted by acetone-dichloromethane or pure acetone solvent system. We can conclude that there is a direct correlation between the cytotoxic effect and amount of taxans.

Keywords

References

  1. Zamani S, Abbasian Z, Khaksar G, Movahedi S, Talebi M, Tabatabaei BES. Genomic diversity among yew (Taxus baccata) genotypes of Iran revealed by random amplified polymorphism DNA markers. International Journal of Agriculture and Biology 2008; 10(6): 648-52.
  2. Khazir J, Mir BA, Mir SA, Cowan D. Natural products as lead compounds in drug discovery. J Asian Nat Prod Res 2013; 15(7): 764-88.
  3. Farina V. The Chemistry and Pharmacology of Taxol and Its Derivatives. Vol. 22. Amsterdam, The Netherland: Elsevier Science Limited; 1995. p. 55-102.
  4. Wada M, Jinno H, Ueda M, Ikeda T, Kitajima M, Konno T, et al. Efficacy of an MPC-BMA co-polymer as a nanotransporter for paclitaxel. Anticancer Res 2007; 27(3B): 1431-5.
  5. DeFuria MD, Horovitz Z. Taxol commercial supply strategy. J Natl Cancer Inst Monogr 1993; (15): 195-8.
  6. Sadeghi-aliabadi H, Asghari G, Mostafavi SA, Esmaeili A. Solvent optimization on Taxol extraction from Taxus baccata L., using HPLC and LC-MS. DARU Journal of Pharmaceutical Sciences 2009; 17(3): 192-8.
  7. Talebi M, Ghassempour A, Talebpour Z, Rassouli A, Dolatyari L. Optimization of the extraction of paclitaxel from Taxus baccata L. by the use of microwave energy. J Sep Sci 2004; 27(13): 1130-6.
  8. Ciccolini J, Catalin J, Blachon MF, Durand A. Rapid high-performance liquid chromatographic determination of docetaxel (Taxotere) in plasma using liquid-liquid extraction. J Chromatogr B Biomed Sci Appl 2001; 759(2): 299-306.
  9. Gangadevi V, Muthumary J. A simple and rapid method for the determination of taxol produced by fungal endophytes from medicinal plants using high performance thin layer chromatography. Se Pu 2008; 26(1): 50-5.
  10. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65(1-2): 55-63.
  11. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 1987; 47(4): 936-42.
  12. Reichman BS, Seidman AD, Crown JP, Heelan R, Yao TJ, Hakes TB, et al. Taxol and recombinant human granulocyte colony-stimulating factor, an active regimen as initial therapy for metastatic breast cancer. A preliminary report. Ann N Y Acad Sci 1993; 698: 398-402.
  13. Hezari M, Croteau R. Taxol biosynthesis: an update. Planta Med 1997; 63(4): 291-5.
  14. Banskota AH, Usia T, Tezuka Y, Kouda K, Nguyen NT, Kadota S. Three new C-14 oxygenated taxanes from the wood of Taxus yunnanensis. J Nat Prod 2002; 65(11): 1700-2.
  15. Bai J, Kitabatake M, Toyoizumi K, Fu L, Zhang S, Dai J, et al. Production of biologically active taxoids by a callus culture of Taxus cuspidata. J Nat Prod 2004; 67(1): 58-63.
  16. Gabetta B, Fuzzati N, Orsini P, Peterlongo F, Appendino G, Vander Velde DG. Paclitaxel analogues from Taxus x media cv. Hicksii. J Nat Prod 1999; 62(2): 219-23.
  17. Chen R, Kingston DG. Isolation and structure elucidation of new taxoids from Taxus brevifolia. J Nat Prod 1994; 57(7): 1017-21.
  18. Javidnia K, Miri R, Amirghofran Z, Jafari A, Amoozegar Z. Cytotoxicity and antimicrobial assessment of Euphorbia hebecarpa. Iran j Pharm Res 2004; 3(2): 75-82.
Journal of Isfahan Medical School
Volume 31, Issue 253 - Serial Number 253
July and August 2013
Pages 1508-1517

Files

History

  • Receive Date: 31 July 2013
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

Share

How to cite

Statistics