تأثیر دو شدت متفاوت تمرینی تناوبی بر بیان ژن پروتئین‌هایBcl-2 وBax بافت عضله‌ی اسکلتی موش‌های صحرایی نر

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکترای فیزیولوژی ورزش، گرایش عصب و عضله، دانشگاه آزاد واحد تهران شمال، تهران، ایران

2 استاد، گروه فیزیولوژی ورزش، واحد محلات، دانشگاه آزاد اسلامی، محلات، ایران

3 دانشیار، گروه فیزیولوژی ورزش، دانشگاه آزاد تهران جنوب، تهران، ایران

چکیده

مقاله پژوهشی




مقدمه: هدف از پژوهش حاضر، بررسی تأثیر دو شدت متفاوت تمرینی تناوبی بر بیان ژن پروتئین‌های Bcl-2 و Bax بافت عضله‌ی اسکلتی موش‌های صحرایی نر بود.
روش‌ها: در مطالعه‌ی تجربی حاضر، 24 سر موش صحرایی نر به صورت تصادفی به سه گروه تمرین تناوبی با شدت بالا، تمرین تناوبی با شدت متوسط و شاهد تقسیم شدند. گروه‌های تمرین به مدت 12 هفته تحت تأثیر تمرین تناوبی با شدت بالا و متوسط قرار گرفتند. پروتکل تمرینی هر دو گروه شامل 12 هفته و 5 روز در هفته بود. به منظور اندازه‌گیری بیان ژن‌هایBcl-2  و Bax از روش Real-time-PCR استفاده شد. ﺩﺍﺩﻩ‌ﻫﺎ ﺑﺎ ﺍﺳﺘﻔﺎﺩه ﺍﺯ ﺁﺯﻣﻮن ﺁﻣﺎری تحلیل واریانس یک‌طرفه ﻭ ﺁﺯﻣﻮﻥ تعقیبی Tukey ﺁﻧﺎﻟﻴﺰ ﺷﺪﻧﺪ.
یافته‌ها: تفاوت معنی‌داری در بیان ژن‌هایBcl-2  عضله‌ی نعلی موش‌های صحرایی نر در سه گروه تمرین تناوبی با شدت بالا، متوسط و شاهد مشاهده شد
(0/001 = P). یافته‌ها نشان داد که بیان ژن Bcl-2 در دو گروه تمرین، به طور معنی‌داری بیشتر از گروه شاهد بود. این در حالی است که بیان ژن  Baxدر سه گروه تفاوت معنی‌داری نداشت (0/154 = P). با این حال بیان این پروتئین در گروه تمرین تناوبی شدید و متوسط به ترتیب 11 و 9 درصد کمتر از گروه شاهد بود.
نتیجه‌گیری: به نظر می‌رسد هر دو شدت تمرین تناوبی بر افزایش پروتئین ضدآپوپتوز میتوکندریایی عضله‌ی نعلی، تأثیر قابل توجهی دارد و نشان از اثر مثبت فعالیت تناوبی در حفظ حیات سلول داشت.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Two Different Intensities of Interval Training on Gene Expression of Bcl-2 and Bax Proteins in Skeletal Muscle Tissue of Male Rats

نویسندگان [English]

  • Mohsen Ghafarimoghadam 1
  • Bahram Abedi 2
  • Abdolali Banaeifar 3
1 PhD. Student in Exercise Physiology, Department of Exercise Physiology, Tehran North Branch, Islamic Azad University, Tehran, Iran
2 Professor, Department of Exercise Physiology, Mahallat Branch, Islamic Azad Univercity, Mahallat, Iran
3 Associate Professor, Department of Exercise Physiology, Tehran North Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Background: The purpose of this study is to investigate the effect of two different intensities of interval training on gene expression of Bcl-2 and Bax proteins in skeletal muscle tissue of male rats.
Methods: In the present experimental study, 24 male rats were randomly divided into three groups: high intensity interval training, moderate intensity interval training and control. The training groups were affected by high and moderate intensity interval training for 12 weeks. The training protocol of both groups included
12 weeks and 5 days per week of High intensity interval training and moderate intensity interval training. Real-time-PCR was obtained to measure the expression of Bcl-2 and Bax genes. Data were analyzed by one-way ANOVA and Tukey's post hoc test.
Findings: Significant differences were observed in the expression of Bcl-2 genes in the skeletal muscle of male rats in three groups (P = 0.001). The results showed that Bcl-2 gene expression in the two exercise groups was significantly higher than the control group. However, Bax gene expression was not significantly different amongst the three groups. The expression of this protein at the group's high intensity and moderate intensity interval training was 11% and 9% lower than the control group respectively.
Conclusion: It seems that both intensities of interval training have a strong impact in increasing the mitochondrial anti-apoptotic protein of the skeletal muscle and revealed the positive effect of interval training on cell survival.

کلیدواژه‌ها [English]

  • Interval training
  • Apoptosis
  • Bcl-2 genes
  • Mitochondria
  • Skeletal muscle

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مجله دانشکده پزشکی اصفهان
دوره 40، شماره 676
هفته 4 مردادماه
مرداد و شهریور 1401
صفحه 451-458

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سابقه مقاله

  • تاریخ دریافت: 01 شهریور 1401
  • تاریخ پذیرش: 01 شهریور 1401

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