تأثیر تمرین تناوبی با شدت بالا همراه با مصرف پودر اسپیرولینا بر تغییرات بیان ژن مسیر التهاب و آتروفی در عضله‌ی نعلی رت‌های مسن چاق مبتلا به دیابت

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری تخصصی، گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه حکیم سبزواری، سبزوار و مربی، گروه علوم ورزشی، دانشکده‌ی ادبیات و علوم انسانی، دانشگاه زابل، زابل، ایران

2 دانشیار، گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه حکیم سبزواری، سبزوار، ایران

چکیده

مقدمه: فعالیت‌های ورزشی، روشی پیشنهادی جهت کاهش التهاب و آتروفی و کنترل بیماری دیابت می‌باشد. اسپیرولینا نیز دارای اثرات ضد التهابی است. هدف از انجام پژوهش حاضر، بررسی تأثیر هشت هفته تمرینات تناوبی با شدت بالا (High-intensity interval training یا HIIT) همراه با مصرف اسپیرولینا، بر تغییرات بیان ژن‌های مسیر التهاب و آتروفی در عضله‌ی نعلی رت‌های مسن چاق مبتلابه دیابت بود.روش‌ها: در این مطالعه‌ی تجربی، 40 سر رت نر مسن نژاد ویستار 20 ماهه با میانگین وزنی 325-280 گرم، پس از خریداری، هشت هفته تحت رژیم پرچرب چاق و پس از آن، با تزریق درون صفاقی 40 میلی‌گرم بر کیلوگرم استرپتوزوسین (Streptozotocin یا STZ) دیابتی شدند. سپس در پنج گروه مساوی (HIIT، HIIT + مکمل، مکمل، شم و شاهد) به مدت هشت هفته و 5 جلسه در هفته، به اجرای HIIT روی تردمیل (با شدت 90 درصد حداکثر اکسیژن مصرفی) در گروه‌های تمرینی و مصرف اسپیرولینا در روزهای تمرین برای گروه‌های HIIT + مکمل و مکمل (50 میلی‌گرم به ازای هر کیلوگرم وزن بدن) پرداختند. بیان ژن‌ها به روش Real-time polymerase chain reaction (Real time-PCR) اندازه‌گیری شد. داده‌ها با استفاده از آزمون‌ One-way ANOVA و آزمون تعقیبی Tukey مورد تجزیه و تحلیل قرار گرفت.یافته‌ها: القای دیابت در رت‌های چاق و مسن، موجب افزایش معنی‌داری در بیان ژن‌های Tweak، Fn14 و Atrogin-1 بین گروه‌های مورد بررسی نسبت به گروه شاهد گردید، اما تمرین و مصرف مکمل این متغیرها را در گروه‌های تمرینی و مکمل نسبت به سایر گروه‌ها به طور معنی‌داری کاهش داد. پس از تمرین، توده‌ی بدنی در گروه‌های HIIT، مکمل و HIIT + مکمل نسبت به گروه‌های شم و شاهد به طور معنی‌داری کاهش یافت و توده‌ی عضله‌ی نعلی نسبت به گروه شاهد تنها در گروه‌های HIIT و HIIT + مکمل، به طور معنی‌داری افزایش داشت.نتیجه‌گیری: با توجه به نتایج تحقیق حاضر، ترکیب HIIT + مکمل به عنوان یک روش مؤثر جهت کاهش آتروفی و افزایش سلامت عضلانی در سالمندان مبتلا به دیابت پیشنهاد می‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of High-Intensity Intermittent Training with Spirulina Powder on Changes in Gene Expression of Inflammation and Atrophy in the Soleus Muscle of Obese Elderly Diabetic Rats

نویسندگان [English]

  • Marzieh Sadat Azarniveh 1
  • Roya Askari 2
  • Amir Hossein Haghighi 2
1 PhD Student, Department of Sports Sciences, University of Hakim Sabzevari, Sabzevar AND Instructor, Department of Sport Sciences, School of Literature and Humanities, University of Zabol, Zabol, Iran
2 Associate Professor, Department of Sports Sciences, University of Hakim Sabzevari, Sabzevar, Iran
چکیده [English]

Background: Exercise is a suggested way to reduce inflammation and atrophy and control diabetes. Spirulina supplement also has anti-inflammatory effects. The aim of this study was to investigate the effect of eight weeks of high-intensity interval training (HIIT) with spirulina on changes in the expression of genes of inflammation and atrophy in the soleus muscle of obese elderly diabetic rats.Methods: In this experimental study, 40 old male Wistar rats (20-month-old) with an average weight of 280-325 g became obese after purchase for 8 weeks on a high-fat diet, and then became diabetic by intraperitoneal injection of streptozocin (40 mg/kg). They were divided in 5 equal groups of HIIT, HIIT + supplement, supplement, sham and control. They did 5 sessions of activities on treadmill with an intensity of 90% VO2max per week for eight weeks to perform HIIT in training groups, and used spirulina consumption (50 mg/kg body weight) as supplementation on training days. Expression of genes were measured using real-time polymerase chain reaction (RT-PCR). One-way ANOVA and Tukey post hoc test were used to analyze the data.Findings: Induction of diabetes significantly increased the expression of TWEAK, Fn14, and atrogin-1 genes in study groups compared to controls; but exercise and supplementation significantly reduced these variables more in exercise and supplementation groups compared to other groups. After training, body mass in HIIT, supplement, and HIIT + supplement groups decreased significantly compared to sham and control groups; and soleus muscle mass increased significantly only in HIIT and HIIT + supplement groups compared to control group.Conclusion: According to the results of HIIT + supplementation, it is recommended as an effective method to reduce atrophy and improve muscle health in diabetic elderly.

کلیدواژه‌ها [English]

  • High-Intensity Interval Training
  • Spirulina
  • Atrophy
  • Cytokine TWEAK
  • Atrogin-1, mouse

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مجله دانشکده پزشکی اصفهان
دوره 39، شماره 652 - شماره پیاپی 652
بهمن و اسفند 1400
صفحه 922-933

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سابقه مقاله

  • تاریخ دریافت: 03 شهریور 1400
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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