Document Type : Original Article (s)
Authors
1
MSc Student of Parasitology, Department of Parasitology and Mycology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
2
Assistant Professor, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3
Professor, Cellular and Molecular Biology Research Center AND Department of Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4
MSc Student of Mycology, Department of Parasitology and Mycology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: Echinococcus granulosus causes human cystic echinococcusis (hydatid cyst) as an important public health problem in many regions of the world. Serologic tests are the main methods for diagnosis of the disease nowadays and enzyme-linked immunosorbent assay (ELISA) is the most routine and common method for diagnosis. There are some problems in primary diagnosis such as cross-reaction with sera from patients with other parasitic disease in serological tests. Using an appropriate source of antigenic material is a very important and crucial point in improvement of ELISA method. Methods: Recombinant AgB Echinococcus granulosus were applied as a new antigen in ELISA and sensitivity and specificity of new kit were compared with Euroimmun hydatid kit. 73 sera from patients with hydatid cyst (confirmed by surgical operation) and 73 healthy individuals’ sera were tested by recombinant AgB kit. Findings: The sensitivity of 100% and specificity of 97.33% were determined by homemade kit. Conclusion: According to our results, recombinant EgAgB has good performance in total IgG ELISA for detection of sera antibodies from patients with hydatid cyst. Keywords: Hydatid cyst, Echinococcus granulosus, Recombinant protein, ELISA, Euroimmun
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