تأثیر تمرین تناوبی شدید و عسل آویشن بر تکثیر و تمایز سلول‌های قلبی رت‌های نر مبتلا به دیابت نوع دو

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 استادیار، گروه تخصصی تربیت بدنی و علوم ورزشی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

3 دانشیار، گروه تخصصی تربیت بدنی و علوم ورزشی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

مقدمه: ورزش و آنتی‌اکسیدان‌ها، به عنوان یک درمان محافظتی در برابر خطر دیابت شناخته می‌شوند. هدف از پژوهش حاضر، بررسی تأثیر تمرین تناوبی شدید و عسل آویشن بر تکثیر و تمایز سلول‌های بافت قلب رت‌های نر مبتلا به دیابت نوع دو بود.
روش‌ها: این مطالعه‌ی تجربی، بر روی 36 رت مبتلا به دیابت انجام شد. رت‌ها به طور تصادفی در چهار گروه شاهد مبتلا به دیابت (8 سر)، تمرین تناوبی (10 سر)، عسل آویشن (8 سر)، تمرین تناوبی‌ و مصرف عسل آویشن (10 سر) تقسیم شدند. مداخله‌ی تمرینی به ‌صورت 8 هفته HIIT، شامل 2 تا 8 تناوب دو دقیقه‌ای دویدن با شدت 80 تا 90 درصد max2VO و استراحت یک دقیقه‌ای با شدت 50 تا 56 درصد max2VO، به مدت 5 جلسه در هفته اجرا می‌شود. علاوه بر این، 3 گرم بر کیلوگرم عسل آویشن، 5 روز در هفته در گروه‌های مکمل مصرف شد. بیان ژن‌های Nkx2.5، Tbx5 و Gata4 توسط real-time RT-PCR ارزیابی گردید. جهت بررسی تفاوت بین گروه‌ها از آزمون ANOVA و آزمون تعقیبی LSD استفاده شد.
یافته‌ها: نتایج نشان داد که بیان ژن Tbx5، Gata4 و Nkx2.5 در گروه HIIT نسبت به گروه شاهد، افزایش معنی‌داری دارد اما این یافته‌ها در شاخص‌های Tbx5 و Nkx2.5 در گروه عسل تکرار نشد (05/0 < P).
نتیجه‌گیری: HIIT با افزایش بیان ژن‌های Nkx2.5، Tbx5 و Gata4 سبب تکثیر و تمایز سلول قلب رت‌های مبتلا به دیابت می‌شد در حالی که تأثیر عسل آویشن و تعامل آن با تمرین، نمی‌توانست مزایای مشابهی در رت‌های مبتلا به دیابت داشته باشد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effects of Interval Exercise and Thyme Honey on Cardiac Cells Proliferation and Differentiation of Type 2 Diabetic Male Rats

نویسندگان [English]

  • Mahdieh Kousha 1
  • Hossein Abednatanzi 2
  • Mandana Gholami 3
  • Farshad Ghazalian 3
1 PhD Student, Department of Physical Education and Sport Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Assistant Professor, Department of Physical Education and Sport Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Associate Professor, Department of Physical Education and Sport Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Background: Exercise and antioxidants are well known for the protection against the risk of diabetes. Therefore, the purpose of the current study was to investigate the effects of intermittent exercise and thyme honey on the proliferation and differentiation of cardiac cells in male rats with type 2 diabetes.
Methods: This experimental study was performed on 36 male diabetic rats which were randomly divided into four groups: control (n = 8), HIIT (n = 10), thyme honey (n = 8) and HIIT*thyme honey (n = 10) groups. The HIIT intervention of 8 weeks, including 2 to 8 of intrmittent running for 2 mins at 80-90% VO2max and 1 min at 50-56% VO2max, was performed for 5 sessions per week. Additionally, 3 g/kg thyme honey was fed, 5 days/week, in supplement groups. Both Nkx2.5 and Tbx5 genes expression in heart tissue was measured by real-time RT-PCR. To evaluate the differences between the groups, ANOVA and LSD post hoc test were used with the significance level of P ≤ 0.05.
Findings: Analysis revealed that expression of Nkx2.5, Gata4 and Tbx5 gene were significantly higher in HIIT compared with the control group whereas these findings were not repeated in Tbx5 and Nkx2.5 indices of the honey group (P > 0.05).
Conclusion: The HIIT is associated with increased expression of Nkx2.5, Tbx5 and Gata4 (1)genes in heart tissue of diabetic rats, while thyme honey did not have similar benefits in diabetic rats.

کلیدواژه‌ها [English]

  • High-Intensity Intermittent Training
  • Diabetes Mellitus
  • Honey
  • Antioxidants
  • Nkx2.5
  • Tbx5
  • Gata4

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مجله دانشکده پزشکی اصفهان
دوره 40، شماره 660
هفته 4 فروردین ماه
فروردین و اردیبهشت 1401
صفحه 76-84

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  • تاریخ دریافت: 03 اردیبهشت 1401
  • تاریخ پذیرش: 03 اردیبهشت 1401

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