نوع مقاله : Original Article(s)
نویسندگان
1 دانشجوی کارشناسی ارشد، گروه انگل و قارچشناسی، دانشکدهی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
2 دکترای تخصصی، گروه ژنتیک و سلولی مولکولی، دانشکدهی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
3 کارشناس مرکز بهداشت استان اصفهان، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
4 دکترای تخصصی، گروه انگل و قارچشناسی، دانشکدهی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background: This study was carried out with the aim of evaluating the rapid diagnostic test (RDT) and microscopic method in comparison with PCR in the detection of Plasmodium vivax parasites in suspected malaria cases.
Methods: In a cross-sectional study, out of 207 febrile patients suspected of malaria, for microscopic diagnosis, blood sample was taken from each person's finger and spread, and after staining with Giemsa dye, were examined with a 100 X optical microscope lens. Whole blood samples were collected from all suspected individuals and rapid diagnostic test (RDT), and also DNA extraction and PCR was performed. To check the validity of different methods, the diagnostic performance (sensitivity, specificity, positive predictive value, and negative predictive value), ROC curve and Kappa coefficient (к) of all three methods was compared.
Findings: In this study, microscopy and RDT compared to PCR have sensitivity of 96% and 94% and specificity of 100% and 98.2%, respectively. Also, the kappa coefficient in the microscopic method is 0.96 and the RDT was 0.91.
Conclusion: Considering the values of sensitivity and specificity, as well as the high kappa coefficient in the microscopic method and RDT, both methods have a very good agreement with the PCR.
کلیدواژهها [English]